X-Meeting presentations

MICROBIOME ANALYSIS OF SHORT-CHAIN FATTY ACIDS SUPPLEMENTATION IN AN EXPERIMENTAL MODEL OF MAJOR DEPRESSIVE DISORDER

Published in 21/11/2024 - ISBN: 978-65-272-0843-3

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Paper Title
MICROBIOME ANALYSIS OF SHORT-CHAIN FATTY ACIDS SUPPLEMENTATION IN AN EXPERIMENTAL MODEL OF MAJOR DEPRESSIVE DISORDER
Authors
  • Luiza Marques Prates Behrens
  • Juciano Gasparotto
  • Lucas Dos Santos Da Silva
  • Marcio Dorn
  • Jose Claudio Fonseca Moreira
Modality
Poster
Subject area
DNA and Genomics
Publishing Date
21/11/2024
Country of Publishing
Brazil | Brasil
Language of Publishing
en-US
Paper Page
https://www.even3.com.br/anais/xmeeting-2024/833133-microbiome-analysis-of-short-chain-fatty-acids-supplementation-in-an-experimental-model-of-major-depressive-disor
ISBN
978-65-272-0843-3
Keywords
Depression, Short-Chain Fatty Acids, Microbiome
Summary
Major Depressive Disorder (MDD) is a complex mental health disorder affecting over 280 million individuals worldwide. In recent years, the microbiota-gut-brain axis has emerged as a promising field for understanding the pathophysiology of MDD. Microbial metabolites such as Short-Chain Fatty Acids (SCFA) - acetate, butyrate, and propionate - have gained attention for their potential role in MDD. However, the precise mechanisms by which these metabolites contribute to MDD pathophysiology remain unclear. This study investigates the effects of oral SCFA supplementation in adult male Wistar rats subjected to Chronic Unpredictable Mild Stress (CUMS), a widely recognized experimental model to study depression-like behavior. A subset of control and CUMS-exposed rats received different supplementations: sodium acetate at a concentration of 60 mM, sodium butyrate at 40 mM, sodium propionate at 50 mM, or a mixture of these SCFAs. The gut microbiome was evaluated through 16S rRNA sequencing of fecal samples collected from five animals in each group using the Ion S5™ System. Alpha diversity was assessed using the Shannon index, and Beta diversity was explored through PCoA based on Bray-Curtis dissimilarity. The LefSe method was employed to detect variations in taxonomic abundance at the genus level. ASV sequences and their abundance were used for predicting metabolic pathways in PICRUSt2. Microbiome analysis revealed notable differences between CUMS-exposed and control groups. Results demonstrated that propionate supplementation significantly alleviated anhedonia in stressed animals, and this ameliorative effect was potentially associated with the modulation of gut bacterial communities, mitigating gut dysbiosis induced by chronic stress. Notably, CUMS-resilient animals exhibited distinct microbial and predicted metabolic profiles resembling those of control animals, indicating resilience-associated gut microbiome patterns. These findings suggest a potential association between gut dysbiosis and stress response, and propionate could be a promising target for future interventions. However, further studies are needed to elucidate the underlying mechanisms of these effects.
Title of the Event
20º Congresso Brasileiro de Bioinformática: X-Meeting 2024
City of the Event
Salvador
Title of the Proceedings of the event
X-Meeting presentations
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

BEHRENS, Luiza Marques Prates et al.. MICROBIOME ANALYSIS OF SHORT-CHAIN FATTY ACIDS SUPPLEMENTATION IN AN EXPERIMENTAL MODEL OF MAJOR DEPRESSIVE DISORDER.. In: X-Meeting presentations. Anais...Salvador(BA) Hotel Deville Prime, 2024. Available in: https//www.even3.com.br/anais/xmeeting-2024/833133-MICROBIOME-ANALYSIS-OF-SHORT-CHAIN-FATTY-ACIDS-SUPPLEMENTATION-IN-AN-EXPERIMENTAL-MODEL-OF-MAJOR-DEPRESSIVE-DISOR. Access in: 26/05/2026

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