MOLECULAR AND CYTOLOGICAL INVESTIGATION OF BACTERIA FROM MYCOPLASMATACEAE FAMILY IN BLOOD SAMPLES FROM DOGS AND CATS TREATED AT A UNIVERSITY VETERINARY HOSPITAL

Larissa Costa dos Santos; Laís da Silva de Oliveira; Victor Silva de Souza; Eduarda de Oliveira Silva Lima Machado; Isaac Leandro Lira Pinto; Camille Barboza Mesquita; Mayara Viera Duarte da Paz; Cristiane Divan Baldani; Huarrisson Azevedo Santos; Maristela Peckle Peixoto

Título do Trabalho

Autores

Larissa Costa dos Santos
Laís da Silva de Oliveira
Victor Silva de Souza
Eduarda de Oliveira Silva Lima Machado
Isaac Leandro Lira Pinto
Camille Barboza Mesquita
Mayara Viera Duarte da Paz
Cristiane Divan Baldani
Huarrisson Azevedo Santos
Maristela Peckle Peixoto

Modalidade

Resumo Simples

Área temática

Protozoários e agentes transmitidos por vetores de importância em saúde

Data de Publicação

22/05/2026

País da Publicação

Brasil

Idioma da Publicação

pt-BR

Página do Trabalho

https://www.even3.com.br/anais/x-simposio-internacional-ciencias-veterinarias/1434416-molecular-and-cytological-investigation-of-bacteria-from-mycoplasmataceae-family-in-blood-samples-from-dogs-and-

ISBN

978-65-272-2456-3

Palavras-Chave

hemoplasma, infection hemoparasitosis

Resumo

Mycoplasmosis is a disease caused by epicellular bacteria of the genus Mycoplasma spp., belonging to the family Mycoplasmataceae, class Mollicutes. Mycoplasmosis is an important cause of hemolytic anemia in felines, although some animals may be asymptomatic carriers. Mycoplasma haemofelis, Candidatus mycoplasma haemominutum, and Candidatus mycoplasma turicensis are the main species, with the first being the most pathogenic. In dogs, mycoplasmosis has been rarely reported and diagnosed in Brazil. Dogs can be affected by two species: Mycoplasma haemocanis, formerly called Haemobartonella canis, and Candidatus Mycoplasma haematoparvum, both causing opportunistic infections in tissues with inflammatory processes resulting from other causes. In addition to anemia, urogenital and respiratory diseases have also been described. The low sensitivity of blood smears for detecting this and other hemoparasitic diseases leads to underdiagnosis and lack of treatment, compromising the clinical improvement of these animals. The objective of this study was to detect bacteria of the Mycoplasmataceae Family using molecular and cytological methods in samples from dogs and cats treated at the University Veterinary Hospital of the Federal Rural University of Rio de Janeiro (HVPA-UFRRJ). A total of 338 blood samples were collected for complete blood count (CBC) analysis at the Veterinary Clinical Pathology Laboratory (LABVET/UFRRJ) and subsequently sent to the Hemoparasites and Vectors Laboratory (LHV/UFRRJ) for storage and processing. Genomic DNA was extracted using the Extracta Kit MGBD-P096 (Loccus, Brazil). The detection of Hemoplasmas from the Mycoplasmataceae family was carried out using the primers MYCO 322S and MYCO938aS with amplification of 620 base pairs. Of the 338 samples analyzed, 4,7% (16/338) amplified DNA for Mycoplasma sp., five from dogs and eleven from cats. In the cytological analysis of the blood smear, no structures suggestive of Mycoplasma sp were observed in red blood cells. No animals positive for Mycoplasma sp. had suspected mycoplasmosis or any typical changes in blood count such as anemia. In this study, PCR proves to be the most sensitive test for detecting this hemoparasitosis.

Título do Evento: X Simpósio Internacional em Ciências Veterinárias | X International Symposium in Veterinary Sciences
Cidade do Evento: Seropédica
Título dos Anais do Evento: Anais do X Simpósio Internacional em Ciências Veterinárias (X SINCVET)
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

SANTOS, Larissa Costa dos et al.. MOLECULAR AND CYTOLOGICAL INVESTIGATION OF BACTERIA FROM MYCOPLASMATACEAE FAMILY IN BLOOD SAMPLES FROM DOGS AND CATS TREATED AT A UNIVERSITY VETERINARY HOSPITAL.. In: Anais do X Simpósio Internacional em Ciências Veterinárias (X SINCVET). Anais...Seropédica(RJ) UFRRJ, 2025. Disponível em: https//www.even3.com.br/anais/x-simposio-internacional-ciencias-veterinarias/1434416-MOLECULAR-AND-CYTOLOGICAL-INVESTIGATION-OF-BACTERIA-FROM-MYCOPLASMATACEAE-FAMILY-IN-BLOOD-SAMPLES-FROM-DOGS-AND-. Acesso em: 09/11/2026