INTERACTION OF THE ENTOMOPATHOGENIC FUNGUS BEAUVERIA BASSIANA WITH EMBRYOS OF THE AQUATIC MODEL ORGANISM DANIO RERIO (ZEBRAFISH)

Haika Victória Sales Moreira; Victor Hugo Machado Luques Dias; José Henrique Vallim; Ana Paula Andrade Braga; Sara Ferreira Abud; Pedro Herculano Santos Silva; Vânia Rita Elias Pinheiro Bittencourt; Patricia Golo; Vera Lúcia Scherholz Salgado de Castro; Isabele da Costa Angelo

Título do Trabalho

Autores

Haika Victória Sales Moreira
Victor Hugo Machado Luques Dias
José Henrique Vallim
Ana Paula Andrade Braga
Sara Ferreira Abud
Pedro Herculano Santos Silva
Vânia Rita Elias Pinheiro Bittencourt
Patricia Golo
Vera Lúcia Scherholz Salgado de Castro
Isabele da Costa Angelo

Modalidade

Resumo Simples

Área temática

Ectoparasitos: sistemática, epidemiologia e controle

Data de Publicação

22/05/2026

País da Publicação

Brasil

Idioma da Publicação

pt-BR

Página do Trabalho

https://www.even3.com.br/anais/x-simposio-internacional-ciencias-veterinarias/1433811-interaction-of-the-entomopathogenic-fungus-beauveria-bassiana-with-embryos-of-the-aquatic-model-organism-danio-r

ISBN

978-65-272-2456-3

Palavras-Chave

Zebrafish, Beauveria bassiana, developmental abnormalities, embryos

Resumo

Zebrafish are widely used model organisms because of their biological transparency, rapid development, and suitability for toxicological studies. Entomopathogenic fungi such as Beauveria bassiana are important biological control agents against arthropod pests and disease vectors and are increasingly used in agriculture under different commercial formulations, including granules, powders, and liquid products. Given their broad application, this study aimed to evaluate the potential risk of the entomopathogenic isolate B. bassiana LCMS21 to Danio rerio embryos in aquatic environments. Conidial suspensions were prepared at 1×10⁸ conidia/mL in zebrafish embryo medium and a serial dilution gave the test concentrations (1×10⁷ to 1×10³ conidia/mL). The Fish Embryo Toxicity Test (FET) was conducted in 24-well plates with 2 mL per well (CEUA–Embrapa Meio Ambiente, protocol nº 002/2025). Embryos (N = 72) were individually exposed to each treatment (fungi concentrations) or to embryo medium (control), and mortality and developmental abnormalities were monitored for four days. Three independent replicates were performed simultaneously. Exposure to B. bassiana LCMS21 did not significantly increase embryo mortality (p = 0.2801). Mortality rates were 10% in the control group, 10% at 1×10³, 14% at 1×10⁴, 8% at 1×10⁵, 3% at 1×10⁶, and 20% at 1×10⁷ conidia/mL. Although the overall frequency of deformities was low (p = 0.0280), some developmental alterations were observed, mainly equilibrium loss (72%), followed by minor spinal/caudal defects (1%) and other abnormalities such as pericardial edema, pigment changes, and contractions (2%). Equilibrium alterations were statistically higher than in the control group at 1×10³ (p = 0.0293), 1×10⁵ (p = 0.0004), 1×10⁶ (p < 0.0001), and 1×10⁷ conidia/mL (p = 0.0004). Overall, mortality did not appear to be a major concern under the tested conditions; however, the occurrence of equilibrium alterations suggests potential sublethal effects of the LCMS21 isolate on early zebrafish development. Further studies are needed to determine whether similar effects occur in later life stages – larvae and adults - or can even be more severe with other fungal isolates.

Título do Evento: X Simpósio Internacional em Ciências Veterinárias | X International Symposium in Veterinary Sciences
Cidade do Evento: Seropédica
Título dos Anais do Evento: Anais do X Simpósio Internacional em Ciências Veterinárias (X SINCVET)
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

MOREIRA, Haika Victória Sales et al.. INTERACTION OF THE ENTOMOPATHOGENIC FUNGUS BEAUVERIA BASSIANA WITH EMBRYOS OF THE AQUATIC MODEL ORGANISM DANIO RERIO (ZEBRAFISH).. In: Anais do X Simpósio Internacional em Ciências Veterinárias (X SINCVET). Anais...Seropédica(RJ) UFRRJ, 2025. Disponível em: https//www.even3.com.br/anais/x-simposio-internacional-ciencias-veterinarias/1433811-INTERACTION-OF-THE-ENTOMOPATHOGENIC-FUNGUS-BEAUVERIA-BASSIANA-WITH-EMBRYOS-OF-THE-AQUATIC-MODEL-ORGANISM-DANIO-R. Acesso em: 09/11/2026