CHARACTERIZATION OF THE FCWF-4 FELINE MACROPHAGE CELL LINE INFECTED WITH THE PARASITE LEISHMANIA AMAZONENSIS AND TREATED WITH GLUCURONOXYLOMANNAN (GXM).

Fernanda de Paula Pepino Moreira; Yuri Alexandre de Carvalho Silveiro; ANA CAROLINA FIGUEIRINHA GOMES; Renata Quintanilha dos Santos; Lucia Mendonça Previato; Elias Barbosa da Silva Junior; Jose Osvaldo Previato; Celio Geraldo freire de Lima; Debora Decote Ricardo de Lima; Lúcia Helena Pinto da Silva

Título do Trabalho

Autores

Fernanda de Paula Pepino Moreira
Yuri Alexandre de Carvalho Silveiro
ANA CAROLINA FIGUEIRINHA GOMES
Renata Quintanilha dos Santos
Lucia Mendonça Previato
Elias Barbosa da Silva Junior
Jose Osvaldo Previato
Celio Geraldo freire de Lima
Debora Decote Ricardo de Lima
Lúcia Helena Pinto da Silva

Modalidade

Resumo Simples

Área temática

Protozoários e agentes transmitidos por vetores de importância em saúde

Data de Publicação

22/05/2026

País da Publicação

Brasil

Idioma da Publicação

pt-BR

Página do Trabalho

https://www.even3.com.br/anais/x-simposio-internacional-ciencias-veterinarias/1431853-characterization-of-the-fcwf-4-feline-macrophage-cell-line-infected-with-the-parasite-leishmania-amazonensis-and

ISBN

978-65-272-2456-3

Palavras-Chave

Leishmaniasis, Fcwf-4, macrophages, GXM, feline

Resumo

Leishmaniasis is a disease caused by parasites of the genus Leishmania. Although it predominantly affects humans and dogs, studies suggest that immunosuppression caused by FIV and FeLV viruses may make felines more susceptible to Leishmania infection, potentially acting as secondary reservoirs of the disease. This study aimed to evaluate the interaction between the parasite Leishmania amazonensis (L. a.) and a feline macrophage cell line (Fcwf-4) in vitro. We analyzed whether the presence of the fungal polysaccharide glucuronoxylomannan (GXM) from Cryptococcus neoformans, which has been shown to inhibit the activity of murine and canine macrophages, could immunomodulate feline Fcwf-4 macrophages during Leishmania infection. Initially, cell viability in the presence or absence of GXM (100 µg/mL) did not show toxicity to Fcwf-4 macrophages infected or not with L. a. Subsequently, Fcwf-4 cells were infected with L. a. In the presence or absence of GXM, the data demonstrated that Fcwf-4 cells can phagocytose L. a. promastigotes and, after 2 hours of interaction, 62% of feline macrophages were infected with L. a.; however, in the presence of GXM, this percentage was 49.03%. On the other hand, in the presence of GXM, 24% of macrophages were infected with L. a. after 48 hours, while in the control group, 10% of Fcwf-4 cells were observed to be infected with L. a. Regarding parasite load, preliminary results showed that feline macrophages treated with GXM presented 41 x 10⁶ parasites/ml, while untreated cells presented 20 x 10⁶/ml. Nitric oxide (NO) production did not differ between treated and untreated cells, nor between LPS-stimulated cells, compared to the control group. The data showed an approximately 1.4-fold increase in ROS production in LPS-stimulated Fcwf-4 cells, and a 1.0-fold decrease in production in GXM-treated cells, compared to control cells. Understanding the biology of feline macrophages is essential for understanding immune responses and disease mechanisms.

Título do Evento: X Simpósio Internacional em Ciências Veterinárias | X International Symposium in Veterinary Sciences
Cidade do Evento: Seropédica
Título dos Anais do Evento: Anais do X Simpósio Internacional em Ciências Veterinárias (X SINCVET)
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

MOREIRA, Fernanda de Paula Pepino et al.. CHARACTERIZATION OF THE FCWF-4 FELINE MACROPHAGE CELL LINE INFECTED WITH THE PARASITE LEISHMANIA AMAZONENSIS AND TREATED WITH GLUCURONOXYLOMANNAN (GXM)... In: Anais do X Simpósio Internacional em Ciências Veterinárias (X SINCVET). Anais...Seropédica(RJ) UFRRJ, 2025. Disponível em: https//www.even3.com.br/anais/x-simposio-internacional-ciencias-veterinarias/1431853-CHARACTERIZATION-OF-THE-FCWF-4-FELINE-MACROPHAGE-CELL-LINE-INFECTED-WITH-THE-PARASITE-LEISHMANIA-AMAZONENSIS-AND. Acesso em: 06/11/2026