Molecular Mechanisms Of The Flavonoid Quercetin In Mimetic Systems Of Oropharyngeal Carcinoma And Mammalian Cell Membranes

Título do Trabalho

MOLECULAR MECHANISMS OF THE FLAVONOID QUERCETIN IN MIMETIC SYSTEMS OF OROPHARYNGEAL CARCINOMA AND MAMMALIAN CELL MEMBRANES

Autores

Giovanna Cisotto Bergamasco Silva (IC); UNESP
Lucas Gontijo Moreira
Karina Alves de Toledo (PD); UNESP
Pedro Aoki

Modalidade

Apresentação - pôster (Clique aqui para saber os detalhes para submissão)

Área temática

Síntese e caracterização de materiais

Data de Publicação

20/05/2022

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/workshopineo2022/473149-molecular-mechanisms-of-the-flavonoid-quercetin-in-mimetic-systems-of-oropharyngeal-carcinoma-and-mammalian-cell-

ISBN

Palavras-Chave

Natural drugs, Flavonoids, Cell membranes, Lipid monolayers, Langmuir films, FTIR

Resumo

Studies with phenolic compounds from natural sources have been more frequent due their relevant pharmacological importance. One of the most abundant flavonoid in vegetables is, quercetin, characterized mainly by its antioxidant, antiviral, and anti-inflammatory activities. These properties are supposedly associated due the way that quercetin interacts with the cell membranes. However, how their molecular mechanisms triggers remains unknown so far. Thus, given the lack of in-depth studies that analyzes the influence of quercetin on membranes, monolayers and multilayers of phospholipids from eukaryotic and tumor membranes were developed in this research, using the Langmuir and Langmuir-Schaefer (LS) film protocol. The phospholipid dipalmitoylphosphatidylcholine (DPPC) is represented here as a simplified healthy model, while the lipid extract from oropharyngeal carcinoma cells (HEp-2) represents a tumor model, which is known to be permissive to viral infections, being relevant for distinct flavonoids actions. Ultrathin films were analyzed regarding the surface pressure measurements caused by the interface molecules versus their occupied area. Such p-A isotherms indicates the adsorption of water-soluble quercetin at the air/water interface, which shows an expansion of the monolayers for higher concentrations of the flavonoid, being more evident in HEp-2 extract films when compared to DPPC. Meanwhile, stability curves were made to measure the behavior of the area occupied by the molecules over time with a fixed pressure of 30 mN/m, which is the same found in the lateral pressure of cell membranes. The results confirms the property of quercetin in establishing adsorption and modifying the fluidity of both DPPC and HEp-2 membranes. Finally, the LS films investigated by Fourier Transformed Infrared Spectrometer (FTIR) provided the visualization of the quecetin binding sites with the membranes, revealing that it can be present in saturated carbons, in addition to the conformational orientations changes founded in phosphates, which affects the surround hydrogen bonds. Such conclusions is promising for developments in the field of therapies involving natural drugs. Acknowledgments: FAPESP (18/22214-6, 20/15854-9) and CNPq (Universal 403713/2016-1, 142243/2021-3) for the financial support.

Título do Evento: Workshop INEO 2022
Título dos Anais do Evento: Anais do Workshop INEO 2022
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

UNESP, Giovanna Cisotto Bergamasco Silva (IC); et al.. MOLECULAR MECHANISMS OF THE FLAVONOID QUERCETIN IN MIMETIC SYSTEMS OF OROPHARYNGEAL CARCINOMA AND MAMMALIAN CELL MEMBRANES.. In: Anais do 16° Workshop do Instituto Nacional de Eletrônica Orgânica. Anais...São Carlos(SP) IFSC, 2022. Disponível em: https//www.even3.com.br/anais/workshopINEO2022/473149-MOLECULAR-MECHANISMS-OF-THE-FLAVONOID-QUERCETIN-IN-MIMETIC-SYSTEMS-OF-OROPHARYNGEAL-CARCINOMA-AND-MAMMALIAN-CELL-. Acesso em: 13/12/2024