INTERACTIONS OF ACETYLENIC ACETOGENINS WITH BIOMIMETIC MEMBRANE MODELS  AT THE AIR-WATER INTERFACE

Gabriel Torquato Berllini; João Henrique Ghilardi Lago; Luciano Caseli

Todos os Trabalhos

Trabalho

Título do Trabalho

INTERACTIONS OF ACETYLENIC ACETOGENINS WITH BIOMIMETIC MEMBRANE MODELS AT THE AIR-WATER INTERFACE

Autores

Gabriel Torquato Berllini
João Henrique Ghilardi Lago
Luciano Caseli

Modalidade

Pôster - resumo

Área temática

Inovação

Data de Publicação

27/05/2026

País da Publicação

Brasil

Idioma da Publicação

pt-BR

Página do Trabalho

https://www.even3.com.br/anais/workshop-do-ineo-2026/1462172-interactions-of-acetylenic-acetogenins-with-biomimetic-membrane-models--at-the-air-water-interface

ISBN

978-65-272-2467-9

Palavras-Chave

Langmuir films, acetylenic acetogenins, biomembranes

Resumo

This study aims to investigate the interactions of a compound composed of an enriched mixture of acetylenic acetogenins, naturally occurring in species of the Annonaceae family and known for their potent cytotoxic and neurotoxic effects [1], as well as their demonstrated pharmacological activity against both trypomastigote and amastigote forms of Trypanosoma cruzi [2], with biomimetic cell membrane models in the form of Langmuir films. The compound is incorporated into lipid monolayers composed of phosphatidylcholine (DOPC and DPPC), phosphatidylethanolamine (DOPE and DPPE), phosphatidylserine (DOPS and DPPS), and cholesterol. Mixed monolayers are formed by co-spreading the compound with the selected lipids at the air–water interface, enabling a detailed investigation of interaction mechanisms between acetylenic acetogenins and membrane models. Monolayer characterization is carried out through surface pressure–area isotherms, compression–expansion (hysteresis) experiments, tensiometric stability measurements, and Brewster angle microscopy analyses. These techniques allow assessment of surface activity and elucidation of the effects of acetogenin incorporation on the structural, thermodynamic, and viscoelastic properties of lipid monolayers. The study is expected to identify lipid classes that are most strongly affected by interactions with acetylenic acetogenins, correlating changes in monolayer organization and mechanical properties with their antiparasitic potential. Furthermore, comparative analyses of monolayers and liposomal systems will provide insight into possible mechanisms of action and assess the feasibility of employing these molecules in selective membrane-targeted delivery strategies. Acknowledgements: FAPESP - processo nº 2025/27044-5 e CNPq - processo: 408449/2024-1 Keywords: Langmuir films, acetylenic acetogenins, biomembranes References: [1] Qazi AK, Siddiqui JA, Jahan R, Chaudhary S, Walker LA, Sayed Z, Jones DT, Batra SK, Macha MA, Emerging therapeutic potential of graviola and its constituents in cancers. Carcinogenesis 39(4) (2018) 522–533 [2] Oliveira, E. A., Brito, I. A., Lima, M. L., Romanelli, M., Moreira-Filho, J. T., Neves, B. J., et al. (2019). Antitrypanosomal Activity of Acetogenins Isolated from the Seeds of Porcelia macrocarpa Is Associated with Alterations in Both Plasma Membrane Electric Potential and Mitochondrial Membrane Potential. Journal of Natural Products.

Título do Evento: Workshop do INEO 2026
Cidade do Evento: Nazaré Paulista
Título dos Anais do Evento: Anais do Workshop do INEO 2026
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

BERLLINI, Gabriel Torquato; LAGO, João Henrique Ghilardi; CASELI, Luciano. INTERACTIONS OF ACETYLENIC ACETOGENINS WITH BIOMIMETIC MEMBRANE MODELS AT THE AIR-WATER INTERFACE.. In: Anais do Workshop do INEO 2026. Anais...Nazaré Paulista(SP) Hotel Estância Atibainha, 2026. Disponível em: https//www.even3.com.br/anais/workshop-do-ineo-2026/1462172-INTERACTIONS-OF-ACETYLENIC-ACETOGENINS-WITH-BIOMIMETIC-MEMBRANE-MODELS--AT-THE-AIR-WATER-INTERFACE. Acesso em: 10/08/2026