DEVELOPMENT OF LANGMUIR–BLODGETT LACCASE/P3HT:OCTADECYLAMINE NANO-ORGANIZED FILMS FOR ELECTROCHEMICAL DETECTION OF PHENOLIC COMPOUNDS

ANDRE CAMPOS MACHADO; Luciano Caseli

Título do Trabalho

Autores

ANDRE CAMPOS MACHADO
Luciano Caseli

Modalidade

Pôster - resumo

Área temática

Sensores e biossensores

Data de Publicação

27/05/2026

País da Publicação

Brasil

Idioma da Publicação

pt-BR

Página do Trabalho

https://www.even3.com.br/anais/workshop-do-ineo-2026/1455253-development-of-langmuirblodgett-laccasep3ht-octadecylamine-nano-organized-films-for-electrochemical-detection-

ISBN

978-65-272-2467-9

Palavras-Chave

Langmuir–Blodgett; laccase; P3HT; octadecylamine; phenolic compounds; electrochemical biosensor.

Resumo

Development of Langmuir–Blodgett laccase/P3HT:octadecylamine nano-organized films for electrochemical detection of phenolic compounds. André C. Machado¹; Maria L. R. Mendez²; Luciano Caseli¹. 1: Federal University of São Paulo (UNIFESP), Institute of Environmental, Chemical and Pharmaceutical Sciences, Diadema, Brazil. 2: Universidad de Valladolid, Valladolid, Spain. acmachado@unifesp.br Phenolic compounds markedly influence the sensory profile and quality of foods and beverages (e.g., wines); however, their quantification in complex matrices typically relies on time-consuming and costly analytical workflows. Here, we report nano-organized Langmuir–Blodgett (LB) architectures for electrochemical sensing of phenolic compounds, employing laccase as the biorecognition/catalytic element and octadecylamine (ODA) and poly(3-hexylthiophene-2,5-diyl) (P3HT) as structuring and conductive components. Interfacial studies were first conducted to tailor monolayer composition and deposition conditions, enabling efficient incorporation of laccase into ODA-based films. Film stability and organization were assessed through surface pressure–area isotherms, surface compressional modulus, hysteresis analysis, kinetic stability, and complementary spectroscopic and morphological techniques. To improve film robustness and suppress P3HT aggregation, mixed P3HT:ODA (1:1) LB films were employed as the immobilization matrix, resulting in quasi-ordered coatings with low surface roughness on ITO substrates. The resulting LB-modified ITO electrodes were evaluated by cyclic voltammetry and square-wave voltammetry toward representative phenolic analytes vanillin (monophenol), catechol (diphenol), and pyrogallol (triphenol), revealing analyte-dependent electrochemical responses. Detection limits of detection of 1.55 × 10-4 M (vanillin) , 2.13 × 10-7 M (catechol), and 2.07 × 10-6 M (pyrogallol) were achieved under optimized conditions, supporting the suitability of LB nano-architectures as reproducible platforms for enzyme-based phenolic sensing. Overall, this approach provides a controllable route to organize biocatalytic interfaces and paves the way for applications in food quality assessment, authenticity monitoring, and process control. References: 1. Girard-Egrot, A. P.; Godoy, S.; Blum, L. J. Advances in Colloid and Interface Science 116 (2005) 205–225. 2. Medina-Plaza, C. et al. Biosensors and Bioelectronics 57 (2014) 276–283.

Título do Evento: Workshop do INEO 2026
Cidade do Evento: Nazaré Paulista
Título dos Anais do Evento: Anais do Workshop do INEO 2026
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

MACHADO, ANDRE CAMPOS; CASELI, Luciano. DEVELOPMENT OF LANGMUIR–BLODGETT LACCASE/P3HT:OCTADECYLAMINE NANO-ORGANIZED FILMS FOR ELECTROCHEMICAL DETECTION OF PHENOLIC COMPOUNDS.. In: Anais do Workshop do INEO 2026. Anais...Nazaré Paulista(SP) Hotel Estância Atibainha, 2026. Disponível em: https//www.even3.com.br/anais/workshop-do-ineo-2026/1455253-DEVELOPMENT-OF-LANGMUIRBLODGETT-LACCASEP3HT-OCTADECYLAMINE-NANO-ORGANIZED-FILMS-FOR-ELECTROCHEMICAL-DETECTION-. Acesso em: 26/07/2026