SYNERGISTIC MEMBRANE INTERACTIONS OF BIOACTIVE ACETOGENINS MIXTURES STUDIED BY LANGMUIR MONOLAYERS

Matheus Elias Rosa; Ivanildo Afonso de Brito; João Henrique Ghilardi Lago; Luciano Caseli

Todos os Trabalhos

Trabalho

Título do Trabalho

SYNERGISTIC MEMBRANE INTERACTIONS OF BIOACTIVE ACETOGENINS MIXTURES STUDIED BY LANGMUIR MONOLAYERS

Autores

Matheus Elias Rosa
Ivanildo Afonso de Brito
João Henrique Ghilardi Lago
Luciano Caseli

Modalidade

Pôster - resumo

Área temática

Materiais e biomateriais

Data de Publicação

27/05/2026

País da Publicação

Brasil

Idioma da Publicação

pt-BR

Página do Trabalho

https://www.even3.com.br/anais/workshop-do-ineo-2026/1454671-synergistic-membrane-interactions-of-bioactive-acetogenins-mixtures-studied-by-langmuir-monolayers

ISBN

978-65-272-2467-9

Palavras-Chave

Langmuir films, natural products, acetogenins, membrane model, neglected tropical diseases

Resumo

Annonaceous Acetogenins (ACGs) are a class of natural products with reported bioactivity against microbial pathogens and tumor cells (1). Two acetylenic ACGs isolated from Porcelia macrocarpa have shown activity against the protozoa Trypanosoma cruzi and Leishmania infantum. Notably, their cytotoxic effects correlate with the degree of unsaturation in their alkyl chains: the more unsaturated compound is biologically active, whereas the more saturated analogues show little or no activity. Furthermore, the mixture of those acetogenins showed increased bioactivity in comparison to their pure forms, highlighting possible synergistic effects between these compounds. Therefore, it is of great importance to study the interactions of these compounds with models of parasitic membranes, gathering insights on their mechanism of action and possible sinergistic effects on membrane binding. This study investigated the interfacial behavior of the mixture of acetogenins (ACGs 1 and 2) alone and with protozoan membrane models based on Langmuir films of DPPE (dipalmitoylphosphatydilethanolamine). Surface pressure-area isotherms, compressional modulus, stability, compression-decompression assays and Brewster Angle Microscopy were executed with films of pure DPPE and with the acetogenins. The results show that unsaturated acetogenins disrupt and destabilize the lipid monolayers through mechanisms distinct from those of the fully saturated compounds. In addition, the unsaturated molecules form filamentous aggregates at surface pressures up to 25 mN/m, promoting significant molecular rearrangements in mixed films. The mixture of ACGs induced changes on the morphology of the DPPE monolayer in comparison to the pure ACGs. The mixture also induced changes on the pressure-area isotherms, stability and rheology. These data indicates that the mixture of ACGs have a synergistic effect that disrupts the membrane models differently from the pure compounds, which might be related to an enhanced interaction with the protozoa membrane, leading to a higher bioactivity. Together, these findings provide mechanistic insight into how structural features such as chain unsaturation influence acetogenin–membrane interactions, helping to clarify their mode of action at biological membranes. Acknowledgements: INEO, Fapesp (2021/15084-1; 2025/27044-5, e CNPq - processo: 408449/2024-1) e CAPES References (1) R. H. Michell, V. L. Heath, & M. V. Taylor. Metabolism of inositol lipids: Biosynthesis and turnover of phosphatidylinositol and phosphatidylinositol-4-phosphate in hepatocytes. Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 644 (1981) 341-350. doi:10.1016/0005-2760(81)90259-4.

Título do Evento: Workshop do INEO 2026
Cidade do Evento: Nazaré Paulista
Título dos Anais do Evento: Anais do Workshop do INEO 2026
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

ROSA, Matheus Elias et al.. SYNERGISTIC MEMBRANE INTERACTIONS OF BIOACTIVE ACETOGENINS MIXTURES STUDIED BY LANGMUIR MONOLAYERS.. In: Anais do Workshop do INEO 2026. Anais...Nazaré Paulista(SP) Hotel Estância Atibainha, 2026. Disponível em: https//www.even3.com.br/anais/workshop-do-ineo-2026/1454671-SYNERGISTIC-MEMBRANE-INTERACTIONS-OF-BIOACTIVE-ACETOGENINS-MIXTURES-STUDIED-BY-LANGMUIR-MONOLAYERS. Acesso em: 26/07/2026