IMPEDIMETRIC IMMUNOSENSOR BASED ON THERMALLY GROWN GOLD NANOPARTICLES

Rafael Cintra Hensel (PD), IFSC-USP; Pedro Ramon Almeida Oiticica; Elsa Materon (PD) - USP; Osvaldo de Oliveira Junior

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Submission

Paper Title

IMPEDIMETRIC IMMUNOSENSOR BASED ON THERMALLY GROWN GOLD NANOPARTICLES

Authors

Rafael Cintra Hensel (PD), IFSC-USP
Pedro Ramon Almeida Oiticica
Elsa Materon (PD) - USP
Osvaldo de Oliveira Junior

Modality

Pôster

Subject area

Biossensores / Biomoléculas

Publishing Date

12/12/2023

Country of Publishing

Brasil

Language of Publishing

Inglês

Paper Page

https://www.even3.com.br/anais/workshop-do-ineo-2023/614276-impedimetric-immunosensor-based-on-thermally-grown-gold-nanoparticles

ISBN

978-65-272-0088-8

Keywords

immunosensor, nanoparticles, biosensor

Summary

Nanostructure engineering presents an important role in the development of sensors due to the deposition of materials with specific properties to enhance or tune the device's performance. To illustrate, the sensitivity and selectivity of such sensors are enhanced by exploiting metallic nanoparticle adsorption [1,2]. Nevertheless, controlling the spatial distribution of nanoparticles while preserving their size and interfaces is still a technological bottleneck. Here, we demonstrate the fabrication of an impedimetric biosensor based on thermally grown gold nanoparticles between interdigitated electrodes (IDEs). A gold film 15 nm thick was evaporated onto gold interdigitated electrodes on glass. Subsequently, the substrates were submitted to thermal annealing that drives the nanoparticle formation. The size and spatial distribution of the nanoparticles between the IDEs were investigated by scanning electron microscopy. Impedance spectroscopy allowed us to keep track of the changes in the device's electric behavior after each modification step. These devices were applied on an impedimetric immunosensor to detect the spike protein of SARS-CoV-2, which presented a lower detection limit compared to the plasmonic version of this platform [3]. A fundamental understanding of the nanoparticles' role in the sensor’s performance might allow the production of enhanced, low-cost, and portable platforms for health monitoring. References [1] Soares, J. C. et al. Immunosensor for pancreatic cancer based on electrospun nanofibers coated with carbon nanotubes or gold nanoparticles. ACS Omega 2, 6975–6983 (2017). [2] Hensel, R. C. et al. Controlled Incorporation of Silver Nanoparticles into Layer-by-Layer Polymer Films for Reusable Electronic Tongues. ACS Appl Nano Mater 4, 14231–14240 (2021). [3] Soares, J. C. et al. Detection of a SARS-CoV-2 sequence with genosensors using data analysis based on information visualization and machine learning techniques. Mater Chem Front 5, 5658–5670 (2021). Acknowledgments The authors are grateful to FAPESP (process 2020/15095-0, 2018/22214-6), and to Angelo Gobbi, Maria H. de O. Piazzetta (LMF/LNNano/CNPEM) for the technical support.

Title of the Event: Workshop do INEO 2023
City of the Event: Nazaré Paulista
Title of the Proceedings of the event: Anais do Workshop INEO 2023
Name of the Publisher: Even3
Means of Dissemination: Meio Digital

How to cite

IFSC-USP, Rafael Cintra Hensel (PD), et al.. IMPEDIMETRIC IMMUNOSENSOR BASED ON THERMALLY GROWN GOLD NANOPARTICLES.. In: Anais do workshop INEO 2023. Anais...Nazaré Paulista(SP) Hotel Estância Atibainha, 2023. Available in: https//www.even3.com.br/anais/workshop-do-ineo-2023/614276-IMPEDIMETRIC-IMMUNOSENSOR-BASED-ON-THERMALLY-GROWN-GOLD-NANOPARTICLES. Access in: 14/10/2024