CHARACTERIZATION OF SILVER NANOPARTICLES SYNTHESIZED BY PULSED LASER ABLATION IN WATER FOR THE ENHANCEMENT OF THE PHOTODYNAMIC ACTIVITY OF ORGANIC PHOTOSENSITIZERS

Paula de Sant’Anna Britto Notari; M. G. J. dos Santos (DR); PUC-Rio; G. Concas (DR); PUC-Rio; Tahir (PD); PUC-Rio; Rafael Dos Santos Carvalho; Marco Cremona; T. Del Rosso (Or); PUC-Rio

Paper Title

Authors

Paula de Sant’Anna Britto Notari
M. G. J. dos Santos (DR); PUC-Rio
G. Concas (DR); PUC-Rio
Tahir (PD); PUC-Rio
Rafael Dos Santos Carvalho
Marco Cremona
T. Del Rosso (Or); PUC-Rio

Modality

Pôster

Subject area

Síntese e caracterização de materiais

Publishing Date

12/12/2023

Country of Publishing

Brasil

Language of Publishing

Inglês

Paper Page

https://www.even3.com.br/anais/workshop-do-ineo-2023/613355-characterization-of-silver-nanoparticles-synthesized-by-pulsed-laser-ablation-in-water-for-the-enhancement-of-the

ISBN

978-65-272-0088-8

Keywords

Silver Nanoparticles; Pulsed Laser Ablation; Photodynamic Therapy; Singlet oxygen.

Summary

In the last decade, photodynamic therapy (PDT) has been recognized as a promising treatment for several types of cancers [1]. The therapy is based on the optical excitation of a proper photosensitizer (PS) which induces the local production of singlet oxygen, followed by the death of the cellular environment. PS-Gold nanoparticles composites have been used so far in order to enhance the ratio between the rate of single oxygen production and the power of the optical excitation [2,3], which is important to untie the application of the PDT from the use of continuous or pulsed laser light sources, and extend to cheapest technologies such as Organic Light Emitting Diodes (OLEDs). In the proposed research we want to investigate the potentialities of photosensitizer-silver nanoparticles composites in the enhancement of the photodynamic activity of methylene blue (MB) [3], exploiting the localized surface plasmon resonance (LSPR) effect. The AgNPs were synthesized by pulsed laser ablation in water (PLAL) [2], where CO2 is introduced into the liquid environment by direct injection or addition of NaOH. Preliminary results show that the introduction of CO2 may produce organic material around the AgNPs, possibly conferring to the nanoparticles specific biological properties, such as an enhanced antimicrobial activity. The synthesized AgNPs were characterized by UV-Vis spectroscopy, ?-potential, inductively coupled plasma mass spectrometry (ICPMS), and transmission electron microscopy (TEM). Changes in the LSPR spectral position, and time stability of the colloidal dispersion of AgNPs, were monitored up to 500 minutes after the addition of a composite photosensitizer consisting of Pluronic F-127 copolymer (PF127) [2] and the photosensitizer methylene blue (MB) [1]. The monitoring has the goal to find the best synthesis conditions, in terms of AgNPs and MB relative concentrations, to obtain an overlap between the LSPR and the absorption band of the photosensitizer. The present research aims at the potential increase of the photodynamic activity of organic photosensitizers based on the nanoantenna properties of the AgNPs/PF127/MB nanocomposites, which should increase the local production of molecular singlet oxygen [3]. In view of potential clinical applications, the use of PF127 may improve the cellular up-take of the nanocomposites, while the presence of silver should prevent infections due to potential antimicrobial activity. Bibliography 1) J.P. Tardivo et Al., “Methylene blue in photodynamic therapy: From basic mechanisms to clinical applications”, Photodiagnosis Photodyn Ther., pp.175-91, 2005 2) T. Del Rosso et al., “Biocompatible Au@Carbynoid/Pluronic-F127 nanocomposites synthesized by pulsed laser ablation assisted CO2 recycling,” Appl. Surf. Sci., vol. 441, pp. 347–355, 2018. 3) T. Simon, M. Potara, A. Gabudean, E. Licarete, M. Banciu, S. Astilean, “Designing theranostic agents based on pluronic stabilized gold nanoaggregates loaded with methylene blue for multimodal cell imaging and enhanced photodynamic therapy”, ACS Appl. Mater. Interfaces 7, pp. 16191–16201, 2015.

Title of the Event: Workshop do INEO 2023
City of the Event: Nazaré Paulista
Title of the Proceedings of the event: Anais do Workshop INEO 2023
Name of the Publisher: Even3
Means of Dissemination: Meio Digital

How to cite

NOTARI, Paula de Sant’Anna Britto et al.. CHARACTERIZATION OF SILVER NANOPARTICLES SYNTHESIZED BY PULSED LASER ABLATION IN WATER FOR THE ENHANCEMENT OF THE PHOTODYNAMIC ACTIVITY OF ORGANIC PHOTOSENSITIZERS.. In: Anais do workshop INEO 2023. Anais...Nazaré Paulista(SP) Hotel Estância Atibainha, 2023. Available in: https//www.even3.com.br/anais/workshop-do-ineo-2023/613355-CHARACTERIZATION-OF-SILVER-NANOPARTICLES-SYNTHESIZED-BY-PULSED-LASER-ABLATION-IN-WATER-FOR-THE-ENHANCEMENT-OF-THE. Access in: 05/10/2024