CONVERGING PHOTODYNAMIC (TFD) AND PHOTOTHERMAL (TFT) THERAPIES ON MELANOMA-DERIVED CELL CULTURES

Mirella Boaro Kobal; Sabrina Alessio Camacho; KARINA ALVES DE TOLEDO; Pedro Aoki

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Submission

Paper Title

CONVERGING PHOTODYNAMIC (TFD) AND PHOTOTHERMAL (TFT) THERAPIES ON MELANOMA-DERIVED CELL CULTURES

Authors

Mirella Boaro Kobal
Sabrina Alessio Camacho
KARINA ALVES DE TOLEDO
Pedro Aoki

Modality

Pôster

Subject area

Síntese e caracterização de materiais

Publishing Date

12/12/2023

Country of Publishing

Brasil

Language of Publishing

Inglês

Paper Page

https://www.even3.com.br/anais/workshop-do-ineo-2023/613292-converging-photodynamic-(tfd)-and-photothermal-(tft)-therapies-on-melanoma-derived-cell-cultures

ISBN

978-65-272-0088-8

Keywords

Photothermal therapy, Photodynamic therapy, Gold nanoparticle, Photosensitizer, Melanoma.

Summary

Cancer is one of the main causes of diseases in the world, being characterized by the uncontrolled growth of cells. Skin cancer, which accounted for 19.3 million cases worldwide in 2020, comprises one-third of all diagnosed cancers. In the search for less invasive clinical treatments, light-based therapies have drawn attention, such as photothermal (PTT) and photodynamic (PDT) therapies. Metallic nanoparticles serve as photothermal agents that convert light into heat, leading to cell death via hyperthermia, while photosensitizers (PS) create reactive oxygen species (ROS) when stimulated by light, causing cell death through oxidative stress. Thus, the combination of PTT and PDT is envisioned as a way to enhance therapeutic efficiency due to the dual effect. Herein, gold nanoparticles were coated with silica (AuSHINs) and then conjugated with the PS methylene violet 3RAX (MV), subsequently received a new layer of silica, forming the AuSHINs_VM_SiO2. UV-Vis spectroscopy revealed that both MV and AuSHINs have their maximum absorption bands in the green region, at 555 nm and 527 nm, respectively. This feature enables the simultaneous activation of PTT and PDT. The spherical shape, monodispersity, and conjugation of AuSHINs with VM were confirmed by dynamic light scattering (DLS), Zeta potential, transmission electron microscopy (TEM), and Raman measurements. Additional measurements were performed to obtain the temperature rise of AuSHINs and AuSHINs_VM_ SiO2 when irradiated at 525nm. The nanostructures and the photosensitizer are also being analyzed for the generation of singlet oxygen (ROS). We are currently assessing the toxicity and phototoxicity of these nanostructures on in vitro cell cultures derived from primary (A375) and metastatic (SH-4) melanoma (skin cancer). In these experiments, we shall evaluate the effectiveness of the functionalized nanostructure (PTT + PDT) against skin cancer cells and compare it to VM (PDT) and AuSHINs (PTT). Additionally, we aim to elucidate the mechanisms of cell death induced by the different photoagents. Acknowledgements: CNPq and FAPESP

Title of the Event: Workshop do INEO 2023
City of the Event: Nazaré Paulista
Title of the Proceedings of the event: Anais do Workshop INEO 2023
Name of the Publisher: Even3
Means of Dissemination: Meio Digital

How to cite

KOBAL, Mirella Boaro et al.. CONVERGING PHOTODYNAMIC (TFD) AND PHOTOTHERMAL (TFT) THERAPIES ON MELANOMA-DERIVED CELL CULTURES.. In: Anais do workshop INEO 2023. Anais...Nazaré Paulista(SP) Hotel Estância Atibainha, 2023. Available in: https//www.even3.com.br/anais/workshop-do-ineo-2023/613292-CONVERGING-PHOTODYNAMIC-(TFD)-AND-PHOTOTHERMAL-(TFT)-THERAPIES-ON-MELANOMA-DERIVED-CELL-CULTURES. Access in: 03/10/2024