Anais do Workshop INEO 2023

DEVELOPMENT OF ORGANIC LIGHT-EMITTING DIODES AS LIGHT SOURCE FOR PHOTODYNAMIC THERAPY ASSAY

Published in 12/12/2023 - ISBN: 978-65-272-0088-8

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Paper Title
DEVELOPMENT OF ORGANIC LIGHT-EMITTING DIODES AS LIGHT SOURCE FOR PHOTODYNAMIC THERAPY ASSAY
Authors
  • Aline Magalhães dos Santos
  • Rafael Dos Santos Carvalho
  • Arthur Rodrigues Jardim Barreto
  • J. P. C. Carmo (IC); PUC-Rio
  • Tomás Barreira Duarte
  • Marco Cremona
Modality
Pôster
Subject area
Dispositivos eletrônicos e ópticos (OLED/OFET/OPVs/etc.)
Publishing Date
12/12/2023
Country of Publishing
Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/workshop-do-ineo-2023/613289-development-of-organic-light-emitting-diodes-as-light-source-for-photodynamic-therapy-assay
ISBN
978-65-272-0088-8
Keywords
Organic Electronics, Photodynamic therapy, biophotonics, photosensitizer, OLED
Summary
One of the most strategic subjects of the century is the research on nanostructured materials for the development of new electronic devices for medical treatments. Among the new materials the family of conjugated organic molecules, which make up the field called Organic Electronics (EO), has shown remarkable success in various applications in nanotechnology and in the development of flexible electronics [1]. In addition, EO is increasingly becoming a multidisciplinary research area. A prominent example is the possibility of using organic light-emitting diodes (OLEDs) [2] produced onto conformable biocompatible substrates as light sources for biophotonic systems. Recently, phototherapy has been calling attention due to recent development in scientific research that involves interactions of an incident photon over a chromophore present in a biological environment. In particular, the photodynamic therapy(PDT) mechanism is a growing field of application of phototherapies with high relevance. The PDT is a non-invasive treatment for surface lesions, such as human epithelial tissue, which uses light to excite a photosensitizer(PS), which is a photosensitive drug. In the absence of irradiation light, the photosensitizer is non-toxic. When irradiated with a specific wavelength the PS molecule can go from the singlet excited state to the triplet excited state, via intersystem crossing. This triplet state can react with the oxygen producing reactive oxygen species(ROS). The ROS produced are reactive and can destroy nearby cells such as bacteria, fungi, and tumor cells. The most relevant species for PDT is singlet oxygen. In PDT treatment, the incident light must be approximately between 600 nm and 1000 nm (therapeutic window) which allows penetration of human tissue into deeper regions, between 5 mm and 20 mm, avoiding invasive actions. In this work, a PDT assay was used to detect the singlet oxygen produced [3] during the irradiation of a photosensitizer solution with a specific probe using an optimized OLED as a light source. We developed three types of OLEDs based on three kinds of emitting layers: Alq_3:DCM2 (fluorescent), BCPO:Ir(fliq)_2 acac and Bebq_2:Ir(pic)_3 (phosphorescent) and mCP:TXO-TPA (TADF). The OLEDs were manufactured by PVD process using glass substrates covered with ITO layer. The PDT assay is performed using a solution with a 10mM of phosphate buffer, 64µM of pluronic F-127, 6µM of methylene blue(MB) as photosensitizer in similar concentrations to PDT treatments and 30µM of 1,3-diphenylisobenzofuran(DPBF) as a probe to detect the singlet oxygen produced by MB in the presence of the OLED light. In the assay, the OLED is placed facing directly the cuvette. The maximum light power density achieved was about 5mW/cm^2 with the OLED based on the Bebq_2:Ir(pic)_3 with a maximum brightness of about 3×10^4 cd/m² at 630nm peak wavelength with 2A/cm² of current density at 12V. The intensity decay in the absorption spectrum of the probe was monitored as a function of the time during the OLED light irradiation[3]. In these conditions, 100% of decay in the absorption spectrum of the probe was measured after 15minutes. Once each probe molecule reacts with one singlet oxygen molecule, this behavior indicates production of at least 30µM of singlet oxygen. This result is comparable with the effect obtained with a commercial LED with 1mW/cm² power density centered at 650nm using the same assay setup. The next step will be to integrate the device onto conformable substrates like bacterial celluloses to be used in the same assay and to develop a more portable light source for photodynamic therapy treatments. References: 1 C. Bizzari et al., Coord Chem. Rev., 373(2018) 2 J. Popp et al., Handbook of biophotonics, Wiley(2012) 3 T. Entradas et al. Journal of photochemistry & photobiology, B: Biology(2020) 4 Y. Jeon et al., ACS Nano,14, 15688-15699(2020)
Title of the Event
Workshop do INEO 2023
City of the Event
Nazaré Paulista
Title of the Proceedings of the event
Anais do Workshop INEO 2023
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

SANTOS, Aline Magalhães dos et al.. DEVELOPMENT OF ORGANIC LIGHT-EMITTING DIODES AS LIGHT SOURCE FOR PHOTODYNAMIC THERAPY ASSAY.. In: Anais do workshop INEO 2023. Anais...Nazaré Paulista(SP) Hotel Estância Atibainha, 2023. Available in: https//www.even3.com.br/anais/workshop-do-ineo-2023/613289-DEVELOPMENT-OF-ORGANIC-LIGHT-EMITTING-DIODES-AS-LIGHT-SOURCE-FOR-PHOTODYNAMIC-THERAPY-ASSAY. Access in: 12/10/2024

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