Anais do Workshop INEO 2023

OPTIMIZATION OF AN ELECTROCHEMICAL BIOSENSOR FOR SAXITOXIN DETECTION IN WATER

Published in 12/12/2023 - ISBN: 978-65-272-0088-8

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Paper Title
OPTIMIZATION OF AN ELECTROCHEMICAL BIOSENSOR FOR SAXITOXIN DETECTION IN WATER
Authors
  • Renê Santos de Amorim
  • Pablo Cesar Serrano Arambulo
  • Gisele Elias Nunes
  • Ivan H Bechtold
Modality
Pôster
Subject area
Biossensores / Biomoléculas
Publishing Date
12/12/2023
Country of Publishing
Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/workshop-do-ineo-2023/613043-optimization-of-an-electrochemical-biosensor-for-saxitoxin-detection-in-water
ISBN
978-65-272-0088-8
Keywords
Biosensor; Saxitoxin; Electroanalysis.
Summary
Saxitoxin is an extremely harmful and naturally produced neurotoxin that is found during water microorganisms/algae bloom season [1]. The Brazilian National Health Surveillance Agency (ANVISA) established a maximum limit of 3.0 µg/L (three micrograms per liter) of this toxin in tap water, showing that even small amounts of this toxin represents serious concern to human Heath [2]. Considering the need of constant monitoring of tapwater quality, and looking for cheap, easy-to-use and fast response methods, this work deals with the optimization of an electrochemical biosensor recently developed in our research group using a single-stranded DNA (called aptamer) for specific recognition of saxitoxin in water coupled with electrochemical impedance spectroscopy (EIS) [3], a non-destructive technique used for analysis of interfacial properties. Preliminary studies demonstrated a safe range of working potential (-0.1 and +0.7 volts when compared with a Ag/AgCl reference electrode), which guarantees the stability of the electrochemical system. Also, that it is possible to obtain a constant baseline value for modified electrodes as long as they are conditioned for a sufficient time in the measurement solutions, alowing aptamers to assume a stable conformation during electrodes building process. Moreover, statistical values of Chi-Square show that the EIS measurements after the time required for equilibration are within ideal conditions for data processing and the tests with different saxitoxin concentrations demonstrated a clear differentiation of signals between the baseline (without toxin) and the signals containing different concentrations. Acknowledgements: INCT/INEO, Capes, CNPq, FAPESC. [1] ETHERIDGE, S. M. Paralytic shellfish poisoning: Seafood safety and human health perspectives. Toxicon, v. 56, n. 2, p. 108–122, ago. 2010. [2] BRASIL. MINISTÉRIO DA SAÚDE. Portaria de Consolidação nº 1, de 28 de setembro de 2017 - Consolidação das normas sobre os direitos e deveres dos usuários da saúde, a organização e o funcionamento do Sistema Único de Saúde. [3] SERRANO, P. C. et al. Electrochemical impedance biosensor for detection of saxitoxin in aqueous solution. Analytical and Bioanalytical Chemistry, v. 413, n. 25, p. 6393–6399, out. 2021.
Title of the Event
Workshop do INEO 2023
City of the Event
Nazaré Paulista
Title of the Proceedings of the event
Anais do Workshop INEO 2023
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

AMORIM, Renê Santos de et al.. OPTIMIZATION OF AN ELECTROCHEMICAL BIOSENSOR FOR SAXITOXIN DETECTION IN WATER.. In: Anais do workshop INEO 2023. Anais...Nazaré Paulista(SP) Hotel Estância Atibainha, 2023. Available in: https//www.even3.com.br/anais/workshop-do-ineo-2023/613043-OPTIMIZATION-OF-AN-ELECTROCHEMICAL-BIOSENSOR-FOR-SAXITOXIN-DETECTION-IN-WATER. Access in: 17/05/2025

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