LIPID PHOTOOXIDATION TRIGGERED BY ROSE BENGAL IN IN VITRO AND BIOINSPIRED SYSTEMS

Published in 12/12/2023 - ISBN: 978-65-272-0088-8

Paper Title
LIPID PHOTOOXIDATION TRIGGERED BY ROSE BENGAL IN IN VITRO AND BIOINSPIRED SYSTEMS
Authors
  • André Satoshi Ferreira
  • Mirella Boaro Kobal
  • Alexandre Mendes de Almeida Junior
  • Lucas Gontijo Moreira
  • KARINA ALVES DE TOLEDO
  • Sabrina Alessio Camacho
  • Pedro Aoki
Modality
Pôster
Subject area
Síntese e caracterização de materiais
Publishing Date
12/12/2023
Country of Publishing
Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/workshop-do-ineo-2023/613024-lipid-photooxidation-triggered-by-rose-bengal-in-in-vitro-and-bioinspired-systems
ISBN
978-65-272-0088-8
Keywords
Langmuir film, colorectal cancer, in vitro culture, photodynamic therapy, xanthene
Summary
Colorectal cancer is currently the third with the highest incidence and the second in the ranking of deaths in the world.1 New therapies are being researched to treat cancer without causing severe side effects. Photodynamic therapy (PDT) is a non-invasive therapeutic approach that uses a photosensitizer (PS) to photo-induce cancer cell death.2 Rose Bengal (RB) is an amphiphilic PS from the xanthene family that has high quantum yield in singlet oxygen (1O2) generation, which is a reactive specie capable of oxidizing biological structures.3 This study assessed the impact of Rose Bengal (RB) on the cell membrane of colorectal carcinoma (Caco-2) through Langmuir films (concentrations ranging from 1 to 25 × 10-6 mol/L), as well as the photodynamic efficiency of RB in different concentrations (0.5 to 25 × 10-6 mol/L) and incubation periods (0.5, 3, and 24 hours) in Caco-2 in vitro cultures. Cell viability was evaluated via MTT assays 24 hours after 1-hour irradiation at 525 nm (32.8 mW/cm2) in in vitro experiments. The Langmuir films results indicate that RB interacts with membrane lipids, leading to their photooxidation upon irradiation and suggesting a possible damage to the cell membrane. MTT assays demonstrated that concentrations above 5 × 10-6 mol/L decreased cell viability by 80%, regardless of the incubation period. Overall, RB has exhibited promising potential as a photosensitizer in the photodynamic therapy of colorectal cancer. Acknowledgments: FAPESP (18/14692-5, 18/22214-6, 20/15854-9, 21/14020-0) and CNPq (Universal 403713/2016-1) for financial support. References 1. Sung, H. et al. Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin 71, 209–249 (2021). 2. Kwiatkowski, S. et al. Photodynamic therapy – mechanisms, photosensitizers and combinations. Biomedicine and Pharmacotherapy vol. 106 1098–1107 Preprint at https://doi.org/10.1016/j.biopha.2018.07.049 (2018). 3. Pellosi, D. S. et al. Photophysical properties and interactions of xanthene dyes in aqueous micelles. J Photochem Photobiol A Chem 247, 8–15 (2012). Autores: A. S. Ferreira (ME); UNESP FCLAssis, M. B. Kobal (DR); UNESP FCLAssis, A. M. de Almeida Júnior (DR); UNESP FCLAssis, L. G. Moreira (ME); UNESP FCLAssis, K. A. de Toledo (PD); UNESP FCLAssis, S. A. Camacho (PD); UNESP FCLAssis and P. H. B. Aoki (Or/Pq); UNESP FCLAssis
Title of the Event
Workshop do INEO 2023
City of the Event
Nazaré Paulista
Title of the Proceedings of the event
Anais do Workshop INEO 2023
Name of the Publisher
Even3
Means of Dissemination
Meio Digital
DOI
LinkGet DOI

How to cite

FERREIRA, André Satoshi et al.. LIPID PHOTOOXIDATION TRIGGERED BY ROSE BENGAL IN IN VITRO AND BIOINSPIRED SYSTEMS.. In: Anais do workshop INEO 2023. Anais...Nazaré Paulista(SP) Hotel Estância Atibainha, 2023. Available in: https//www.even3.com.br/anais/workshop-do-ineo-2023/613024-LIPID-PHOTOOXIDATION-TRIGGERED-BY-ROSE-BENGAL-IN-IN-VITRO-AND-BIOINSPIRED-SYSTEMS. Access in: 25/07/2024

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