DEVELOPMENT AND CATALYTIC ACTIVITY OF HORSERADISH PEROXIDASE AND CONJUGATED POLYMER THIN FILMS

C. G. de Jesus (IC); Unifesp; R. R. Rodrigues (DR); Unifesp; Luciano Caseli; Laura Péres

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Submission

Paper Title

DEVELOPMENT AND CATALYTIC ACTIVITY OF HORSERADISH PEROXIDASE AND CONJUGATED POLYMER THIN FILMS

Authors

C. G. de Jesus (IC); Unifesp
R. R. Rodrigues (DR); Unifesp
Luciano Caseli
Laura Péres

Modality

Pôster

Subject area

Biossensores / Biomoléculas

Publishing Date

12/12/2023

Country of Publishing

Brasil

Language of Publishing

Inglês

Paper Page

https://www.even3.com.br/anais/workshop-do-ineo-2023/612929-development-and-catalytic-activity-of-horseradish-peroxidase-and-conjugated-polymer-thin-films

ISBN

978-65-272-0088-8

Keywords

biosensor, peroxidase, enzyme kinetics, conjugated polymer

Summary

It is known that several molecules play various roles to maintain the human organism. However, excess molecules such as hydrogen peroxide may oxidize proteins, DNA, and other compounds, causing diseases. In this regard, the detection of hydrogen peroxide by horseradish peroxidase (HRP) biosensors has been widely used due to the quick response and reusability of these devices [1]. The applicability of biosensors depends on the immobilization of the enzyme, which makes conjugated polymers (CPs) interesting material for that function. Due to the presence of alternated double bonds along the chains, these polymers have optoelectrical properties, being used in developing devices such as OLEDs and solar cells [2]. The present work brings a study of how conjugated polymers with different functional groups may affect the catalytic activity of HRP-based thin films. In this sense, the spin coating technique was used to produce HRP/CP thin films containing three different polymers. The films were immersed in a reactional solution containing hydrogen peroxide and the absorbance increase in 420 nm was monitored to determine the catalytic activity and the Michaelis- Menten (Km) constant. As the main results, we could observe that the CP with a higher molar mass had a higher Km value, confirming the point observed in previous work [3]. However, unlike what was expected, for the two remaining polymers, the one with a higher molar mass had a better Km value, which probably indicates the interaction between the CP and the electroactive site of HRP. Acknowledgments: This work was supported by INEO, CNPq (310647/2020-7), Capes, and FAPESP (grant number 2020/04427-2, 2014/50869-6). References: [1] Goto, T. E., Lopez, R. F., Oliveira, O. N., & Caseli, L. (2010). Enzyme activity of catalase immobilized in Langmuir-Blodgett films of phospholipids. Langmuir, 26(13), 11135–11139. [2] Schmidt, T. F., Caseli, L., dos Santos, D. S., & Oliveira, O. N. (2009). Enzyme activity of horseradish peroxidase immobilized in chitosan matrices in alternated layers. Materials Science and Engineering C, 29(6), 1889–1892. [3] Jesus, C. G. De, Rodrigues, R., Caseli, L., & P, L. O. (2022). Colloids and Surfaces A: Physicochemical and Engineering Aspects Conducting polymers modulating the catalytic activity of urease in thin composite films. 654(130136).

Title of the Event: Workshop do INEO 2023
City of the Event: Nazaré Paulista
Title of the Proceedings of the event: Anais do Workshop INEO 2023
Name of the Publisher: Even3
Means of Dissemination: Meio Digital

How to cite

UNIFESP, C. G. de Jesus (IC); et al.. DEVELOPMENT AND CATALYTIC ACTIVITY OF HORSERADISH PEROXIDASE AND CONJUGATED POLYMER THIN FILMS.. In: Anais do workshop INEO 2023. Anais...Nazaré Paulista(SP) Hotel Estância Atibainha, 2023. Available in: https//www.even3.com.br/anais/workshop-do-ineo-2023/612929-DEVELOPMENT-AND-CATALYTIC-ACTIVITY-OF-HORSERADISH-PEROXIDASE-AND-CONJUGATED-POLYMER-THIN-FILMS. Access in: 18/05/2025