ANTIPARASITIC ACETOGENIN INTERACTION WITH DPPE MEMBRANE AT THE AIR-WATER INTERFACE

Matheus Elias Rosa; Luciano Caseli; Matheus Elias Rosa

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Paper Title

ANTIPARASITIC ACETOGENIN INTERACTION WITH DPPE MEMBRANE AT THE AIR-WATER INTERFACE

Authors

Matheus Elias Rosa
Luciano Caseli
Matheus Elias Rosa

Modality

Pôster

Subject area

Biossensores / Biomoléculas

Publishing Date

12/12/2023

Country of Publishing

Brasil

Language of Publishing

Inglês

Paper Page

https://www.even3.com.br/anais/workshop-do-ineo-2023/610747-antiparasitic-acetogenin-interaction-with-dppe-membrane-at-the-air-water-interface

ISBN

978-65-272-0088-8

Keywords

Natural Products, Biomembranes, Biomolecules, Langmuir monolayers, Acetogenins, Antitumoral, Antiparasitic, Neglected Tropical Diseases

Summary

Acetogenins are a class of biomolecules extracted from Annonaceae family plants. Many of these compounds have shown cytotoxicity, antiparasitic and antitumoral activities. However, its mechanism of action is not yet elucidated: the drug might act by penetrating the cell membrane structure, interacting with intracellular organelles, or by interacting with the cell membrane, provoking alterations in its physical-chemical properties, such as fluidity, viscoelasticity, or stability. Recently, acetylenic acetogenins were extracted from Porcelia macrocarpa seeds and presented activity against different forms of Trypanosoma cruzi protozoan, and it was observed that saturation on the aliphatic chain of the compound greatly influences the bioactivity of the drug. Therefore, to investigate the interaction of saturated acetogenins with cell membranes, they were tested against Langmuir films. These structures are monolayers oriented at the air-water interface composed of an amphiphilic molecule and can be used as a cell-membrane model if phospholipids are employed to evaluate their interaction with bioactive molecules. In this work, we studied the interaction of PMD3, a saturated acetogenin, with Langmuir monolayers composed of dipalmitoyl phosphatidyl ethanolamine (DPPE), a phospholipid well present in microbial cell membranes. The films were characterized by surface pressure - area (p-A) isotherms, compression-decompression cycles, polarization-modulated infrared reflection-absorption spectroscopy (PM-IRRAS), tensiometric stability assays, and Brewster angle microscopy (BAM). By introducing the PMD3 drug into the DPPE monolayer, we observed a shift of the isotherm curve to higher areas, indicating the film's expansion by incorporation of the drug. The drug also increased the degree of hysteresis and stability of the monolayer at the surface pressure of 30 mN/m, which is the lateral pressure observed in most natural cell membranes. PM-IRRAS spectra show the interaction of PMD3 with the alkylic chain of DPPE and BAM images, indicating a homogeneous distribution of the mixed film along the air-water interface. Thus, we conclude that acetogenin affects the thermodynamical and rheological properties of the DPPE film, further elaborating on the possible mechanism of action of the drug in parasitic cells. Acknowledgments: FAPESP, CNPq, CAPES

Title of the Event: Workshop do INEO 2023
City of the Event: Nazaré Paulista
Title of the Proceedings of the event: Anais do Workshop INEO 2023
Name of the Publisher: Even3
Means of Dissemination: Meio Digital
DOI: Get DOI

How to cite

ROSA, Matheus Elias; CASELI, Luciano; ROSA, Matheus Elias. ANTIPARASITIC ACETOGENIN INTERACTION WITH DPPE MEMBRANE AT THE AIR-WATER INTERFACE.. In: Anais do workshop INEO 2023. Anais...Nazaré Paulista(SP) Hotel Estância Atibainha, 2023. Available in: https//www.even3.com.br/anais/workshop-do-ineo-2023/610747-ANTIPARASITIC-ACETOGENIN-INTERACTION-WITH-DPPE-MEMBRANE-AT-THE-AIR-WATER-INTERFACE. Access in: 27/07/2024