Congresso de Genética do Centro-Oeste

IDENTIFICATION OF ACINETOBACTER BAUMANNII ISOLATES USING MATRIX-ASSISTED LASER DESORPTION/IONIZATION TIME-OF-FLIGHT MASS SPECTROMETRY (MALDI-TOF MS)

Publicado em 03/09/2025 - ISBN: 978-65-272-1473-1

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Título do Trabalho
IDENTIFICATION OF ACINETOBACTER BAUMANNII ISOLATES USING MATRIX-ASSISTED LASER DESORPTION/IONIZATION TIME-OF-FLIGHT MASS SPECTROMETRY (MALDI-TOF MS)
Autores
  • José Eduardo Souza Echeverria
  • Pedro Julio Franco Camilo
  • Sophia Aléo Bomfim
  • Marielly lopes Ferreira
  • Monyque Palagano da Rocha
  • Gleyce Hellen de Almeida de Souza
  • SIMONE SIMIONATTO
Modalidade
Resumo
Área temática
Genética de Microrganismos
Data de Publicação
03/09/2025
País da Publicação
Brasil
Idioma da Publicação
pt-BR
Página do Trabalho
https://www.even3.com.br/anais/vicgco/1190037-identification-of-acinetobacter-baumannii-isolates-using-matrix-assisted-laser-desorptionionization-time-of-fli
ISBN
978-65-272-1473-1
Palavras-Chave
Antimicrobial resistance, carbapenems, bacterial identification, epidemiological surveillance.
Resumo
Acinetobacter baumannii is a critically prioritized nosocomial pathogen, frequently associated with severe infections and widely recognized for its extensive antimicrobial resistance. Accurate identification of clinical microorganisms is essential for infection control and for selecting appropriate antimicrobial therapies. Automated systems such as BD Phoenix® are widely used due to their reliability and cost-effectiveness. However, alternative methods like matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) have gained prominence for their speed and higher accuracy in microbial identification. This study aimed to reassess clinical isolates previously identified as A. baumannii by the BD Phoenix® automated system, using MALDI-TOF-MS. A total of 112 strains from hospitalized patients were analyzed by the MALDI-TOF-MS. Results were interpreted based on score values: ≥ 2.00 indicated reliable species-level identification; between 1.70 and 1.99, genus-level identification; and < 1.70, unreliable identification. Among the isolates, 94.6% (n = 106) were reliably identified as A. baumannii at the species level, 4.4% (n = 5) at the genus level, and 0.9% (n = 1) yielded unreliable identification. The isolates are currently undergoing whole-genome sequencing (WGS) to map genes associated with antimicrobial resistance. The high concordance between MALDI-TOF-MS and the automated system reinforces the effectiveness of both approaches. MALDI-TOF-MS stands out for its rapidity, representing a strategic tool to expedite therapeutic decisions in clinical settings.
Título do Evento
VI Congresso de Genética do Centro-Oeste
Cidade do Evento
Campo Grande
Título dos Anais do Evento
Congresso de Genética do Centro-Oeste
Nome da Editora
Even3
Meio de Divulgação
Meio Digital

Como citar

ECHEVERRIA, José Eduardo Souza et al.. IDENTIFICATION OF ACINETOBACTER BAUMANNII ISOLATES USING MATRIX-ASSISTED LASER DESORPTION/IONIZATION TIME-OF-FLIGHT MASS SPECTROMETRY (MALDI-TOF MS).. In: Congresso de Genética do Centro-Oeste. Anais...Campo Grande(MS) UFMS, 2025. Disponível em: https//www.even3.com.br/anais/vicgco/1190037-IDENTIFICATION-OF-ACINETOBACTER-BAUMANNII-ISOLATES-USING-MATRIX-ASSISTED-LASER-DESORPTIONIONIZATION-TIME-OF-FLI. Acesso em: 09/06/2026

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