EVALUATION OF THE IN VITRO INTERACTION OF PBMCS FROM RRMS PATIENTS WITH AN OLIGODENDROCYTE PRECURSOR CELL LINE

Publicado em 09/06/2023 - ISBN: 978-85-5722-785-9

Título do Trabalho
EVALUATION OF THE IN VITRO INTERACTION OF PBMCS FROM RRMS PATIENTS WITH AN OLIGODENDROCYTE PRECURSOR CELL LINE
Autores
  • Natália Munhoz Alves
  • Natália Brunetti Silva
  • Rodrigo Miranda de Carvalho
  • Raphael Patricio da Silva Quintiliano
  • Breno Bandoni Ferrari
  • Vinícius Oliveira Boldrini
  • Alessandro Faria
Modalidade
Apresentação de Pôster
Área temática
Imunologia
Data de Publicação
09/06/2023
País da Publicação
Brasil
Idioma da Publicação
Inglês
Página do Trabalho
https://www.even3.com.br/anais/v-gbmeeting/609818-evaluation-of-the-in-vitro-interaction-of-pbmcs-from-rrms-patients-with-an-oligodendrocyte-precursor-cell-line
ISBN
978-85-5722-785-9
Palavras-Chave
Multiple sclerosis, Cytotoxic CD4 T lymphocytes, Oligodendrocytes precursor cells
Resumo
Multiple sclerosis (MS) is an inflammatory and autoimmune disease of the central nervous system (CNS). The cytotoxic activity of CD4+ T lymphocytes is well documented in tumor-specific and antiviral immune responses and has been reported as an effector mechanism during MS development. Moreover, studies have shown that cytotoxic CD4+ T cells (CD4_CTLs) drive MS progression and contribute to MS lesions by targeting oligodendrocytes. Regarding the experimental model of MS, the expression of granzyme B (GzmB) by encephalitogenic CD4+ T cells has been described as essential for breaching the blood-brain barrier. Additionally, the treatment with a GzmB-inhibitor – serpina3n – decreased the severity of EAE, although it did not affect infiltration of CD4+ or CD8+ T cells into the CNS. Furthermore, CD4_CTLs alone were able to induce apoptosis in healthy mice brain tissue. However, until the present moment, it is unclear whether such cytotoxic activity is induced by the direct interaction of CD4_CTLs and oligodendrocytes or if these cells interact with local antigen-presenting cells and release cytotoxic molecules into the CNS milieu. To resolve this uncertainty, we first aim to evaluate whether the indirect interaction with these cells can induce apoptosis in oligodendrocytes during MS. In order to accomplish that, peripheral blood mononuclear cells (PBMCs) previously isolated from relapsing-remitting MS patients (RRMS) and healthy controls will be stimulated with anti-CD3 and anti-CD28 antibodies, and co-cultured with a oligodendrocyte precursor cell lineage (MO3.13) through a transwell system. Subsequently, we will assess the apoptosis induction in the oligodendrocyte precursor cells by immunofluorescence and collect the co-culture supernatant to quantify GzmB production levels. Based on the described methodoloy, our goal is to determine whether the CD4+ T lymphocyte cytotoxic activity over oligodendrocytes observed during MS neuroinflammation may or may not require direct contact between both cell types.
Título do Evento
V GBMeeting
Cidade do Evento
Campinas
Título dos Anais do Evento
Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora
Even3
Meio de Divulgação
Meio Digital
DOI
LinkObter o DOI

Como citar

ALVES, Natália Munhoz et al.. EVALUATION OF THE IN VITRO INTERACTION OF PBMCS FROM RRMS PATIENTS WITH AN OLIGODENDROCYTE PRECURSOR CELL LINE.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609818-EVALUATION-OF-THE-IN-VITRO-INTERACTION-OF-PBMCS-FROM-RRMS-PATIENTS-WITH-AN-OLIGODENDROCYTE-PRECURSOR-CELL-LINE. Acesso em: 12/06/2024

Trabalho

Even3 Publicacoes