EVALUATION OF THE IN VITRO INTERACTION OF PBMCS FROM RRMS PATIENTS WITH AN OLIGODENDROCYTE PRECURSOR CELL LINE

Natália Munhoz Alves; Natália Brunetti Silva; Rodrigo Miranda de Carvalho; Raphael Patricio da Silva Quintiliano; Breno Bandoni Ferrari; Vinícius Oliveira Boldrini; Alessandro Faria

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Trabalho

Título do Trabalho

EVALUATION OF THE IN VITRO INTERACTION OF PBMCS FROM RRMS PATIENTS WITH AN OLIGODENDROCYTE PRECURSOR CELL LINE

Autores

Natália Munhoz Alves
Natália Brunetti Silva
Rodrigo Miranda de Carvalho
Raphael Patricio da Silva Quintiliano
Breno Bandoni Ferrari
Vinícius Oliveira Boldrini
Alessandro Faria

Modalidade

Apresentação de Pôster

Área temática

Imunologia

Data de Publicação

09/06/2023

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/v-gbmeeting/609818-evaluation-of-the-in-vitro-interaction-of-pbmcs-from-rrms-patients-with-an-oligodendrocyte-precursor-cell-line

ISBN

978-85-5722-785-9

Palavras-Chave

Multiple sclerosis, Cytotoxic CD4 T lymphocytes, Oligodendrocytes precursor cells

Resumo

Multiple sclerosis (MS) is an inflammatory and autoimmune disease of the central nervous system (CNS). The cytotoxic activity of CD4+ T lymphocytes is well documented in tumor-specific and antiviral immune responses and has been reported as an effector mechanism during MS development. Moreover, studies have shown that cytotoxic CD4+ T cells (CD4_CTLs) drive MS progression and contribute to MS lesions by targeting oligodendrocytes. Regarding the experimental model of MS, the expression of granzyme B (GzmB) by encephalitogenic CD4+ T cells has been described as essential for breaching the blood-brain barrier. Additionally, the treatment with a GzmB-inhibitor – serpina3n – decreased the severity of EAE, although it did not affect infiltration of CD4+ or CD8+ T cells into the CNS. Furthermore, CD4_CTLs alone were able to induce apoptosis in healthy mice brain tissue. However, until the present moment, it is unclear whether such cytotoxic activity is induced by the direct interaction of CD4_CTLs and oligodendrocytes or if these cells interact with local antigen-presenting cells and release cytotoxic molecules into the CNS milieu. To resolve this uncertainty, we first aim to evaluate whether the indirect interaction with these cells can induce apoptosis in oligodendrocytes during MS. In order to accomplish that, peripheral blood mononuclear cells (PBMCs) previously isolated from relapsing-remitting MS patients (RRMS) and healthy controls will be stimulated with anti-CD3 and anti-CD28 antibodies, and co-cultured with a oligodendrocyte precursor cell lineage (MO3.13) through a transwell system. Subsequently, we will assess the apoptosis induction in the oligodendrocyte precursor cells by immunofluorescence and collect the co-culture supernatant to quantify GzmB production levels. Based on the described methodoloy, our goal is to determine whether the CD4+ T lymphocyte cytotoxic activity over oligodendrocytes observed during MS neuroinflammation may or may not require direct contact between both cell types.

Título do Evento: V GBMeeting
Cidade do Evento: Campinas
Título dos Anais do Evento: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora: Even3
Meio de Divulgação: Meio Digital
DOI: Obter o DOI

Como citar

ALVES, Natália Munhoz et al.. EVALUATION OF THE IN VITRO INTERACTION OF PBMCS FROM RRMS PATIENTS WITH AN OLIGODENDROCYTE PRECURSOR CELL LINE.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609818-EVALUATION-OF-THE-IN-VITRO-INTERACTION-OF-PBMCS-FROM-RRMS-PATIENTS-WITH-AN-OLIGODENDROCYTE-PRECURSOR-CELL-LINE. Acesso em: 27/07/2024