EVALUATION OF THE INFLUENCE OF THE RUBBER TREE (HEVEA BRASILIENSIS) ROOTSTOCK ON THE GENE EXPRESSION OF THE GRAFTED CLONE.

Vinicius Mandolesi Rios; Anete Pereira de Souza

Título do Trabalho

Autores

Vinicius Mandolesi Rios
Anete Pereira de Souza

Modalidade

Apresentação de Pôster

Área temática

Genética Vegetal e Melhoramento

Data de Publicação

09/06/2023

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/v-gbmeeting/609790-evaluation-of-the-influence-of-the-rubber-tree-(hevea-brasiliensis)-rootstock-on-the-gene-expression-of-the-graft

ISBN

978-85-5722-785-9

Palavras-Chave

Rubber tree, rootstock, RNA-seq

Resumo

Introduction: Natural rubber is an essential raw material for products in several industries. Its only commercial source is the rubber tree (Hevea brasiliensis Muell. Arg.). Although it is not native to the southeastern region of Brazil, the state of São Paulo is currently the largest producer due to the colder climate that makes it difficult to spread the agent that causes leaf blight, the main pest of the rubber tree. These trees, when planted by seed, show significant variation in latex production; to improve production, grafting is used, in which only the most productive clones are propagated asexually. This method reduces the variability of the production. However, this method does not consolidate a totally uniform production, even under the same climatic conditions. The observed variation is most likely due to the influence of the rootstock genotype, since it is variable because it is the fruit of seeds. Many studies evaluate the influence of the rootstock in several species, using the RNA-seq methodology to elucidate the molecular mechanisms involved. To date, no studies have evaluated the influence of different rootstocks on rubber trees using transcriptomic analyses. A better understanding of the molecular mechanisms involved in these interactions may allow the development of new approaches to improve rubber trees, with rootstock selection being an unexplored path to increase productivity. Objectives: The present study aims to assemble the transcriptome of the most used clone in the State of São Paulo, RRIM 600, and its different rootstocks in order to identify genes, metabolic pathways and biological processes associated with the interaction of grafting in rubber trees. Methods: Scion and rootstock trunk tissues of RRIM 600 clones grafted onto plants from seeds of GT 1, PB 235 and RRIM 600 clones will be collected from a 30-year field experiment at the Pindorama Experimental Station of Agronomy. Samples will be genotyped using microsatellite markers. RNA will be extracted and sequenced following RNA-seq methodologies. Results will be validated by qRT-PCR.

Título do Evento: V GBMeeting
Cidade do Evento: Campinas
Título dos Anais do Evento: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora: Even3
Meio de Divulgação: Meio Digital
DOI: Obter o DOI

Como citar

RIOS, Vinicius Mandolesi; SOUZA, Anete Pereira de. EVALUATION OF THE INFLUENCE OF THE RUBBER TREE (HEVEA BRASILIENSIS) ROOTSTOCK ON THE GENE EXPRESSION OF THE GRAFTED CLONE... In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609790-EVALUATION-OF-THE-INFLUENCE-OF-THE-RUBBER-TREE-(HEVEA-BRASILIENSIS)-ROOTSTOCK-ON-THE-GENE-EXPRESSION-OF-THE-GRAFT. Acesso em: 27/07/2024