THE ROLE OF INNATE RECOGNITION PATHWAYS AND TYPE I AND III INTERFERONS FOR MAINTENANCE OF THE BLOOD-PLACENTAL BARRIER AFTER OROPOUCHE VIRUS INFECTION

Stefanie Primon Muraro; Gabriela Fabiano de Souza; Julia Forato; Matheus Cavalheiro Martini; José Modena

Título do Trabalho

Autores

Stefanie Primon Muraro
Gabriela Fabiano de Souza
Julia Forato
Matheus Cavalheiro Martini
José Modena

Modalidade

Apresentação Oral

Área temática

Imunologia

Data de Publicação

09/06/2023

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/v-gbmeeting/609780-the-role-of-innate-recognition-pathways-and-type-i-and-iii-interferons-for-maintenance-of-the-blood-placental-bar

ISBN

978-85-5722-785-9

Palavras-Chave

Oropouche virus; placenta; arbovirus

Resumo

The Oropouche virus (OROV) is an arbovirus with potential to cause epidemics in the most populated regions of Brazil. Individuals infected with OROV develop a febrile illness, which can progress to neurological and hemorrhagic complications. Furthermore, an increased incidence of abortion was reported during major OROV epidemics. However, the pathogenetic mechanisms associated with the hematoplacental barrier breakdown during OROV infection have not yet been investigated. For this, we infected mice, and trophoblasts cell lines with OROV in order to characterize the routes of signaling and antiviral response induced by this infection in villous and extravilous trophoblasts. We verified that OROV infection of trophoblasts leads to the activation of receptors capable of recognizing the viral genome and induces the expression of type I and III IFNs. In addition, embryos and placenta from OROV-infected mice on the embrionary day (E) 10 showed signals of viral infection and immune activation when they were euthanatized on E16.5, particularly pronounced in IFNAR knockout animals with reduction in its size and weight. By qPCR we found out that IFNAR knockout placentas have high levels of IFNa with ISGs activation (OASL and IFIT1). Though is dependent on interferon type I signaling, it seems independent of Myd88 and MAVS adaptors once we didn`t see any difference in viral loads. When we used IL28Ra knockout mice we also didn`t see any differences in viral load and phenotype compared to WT mice at E16.5. Finally, to examine possible abnormalities in the placenta that would lead to damage of the fetus we analyzed glycolytic genes that are present in hypoxia. We found high levels of both PKM2 and PFKFB3 enzymes and also Glut1, Hif1a and IL1ß on the placenta of IFNAR knockout infected animals. In conclusion, OROV can cross the placental barrier and affect fetal development dependent on IFNa receptor response.

Título do Evento: V GBMeeting
Cidade do Evento: Campinas
Título dos Anais do Evento: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora: Even3
Meio de Divulgação: Meio Digital
DOI: Obter o DOI

Como citar

MURARO, Stefanie Primon et al.. THE ROLE OF INNATE RECOGNITION PATHWAYS AND TYPE I AND III INTERFERONS FOR MAINTENANCE OF THE BLOOD-PLACENTAL BARRIER AFTER OROPOUCHE VIRUS INFECTION.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609780-THE-ROLE-OF-INNATE-RECOGNITION-PATHWAYS-AND-TYPE-I-AND-III-INTERFERONS-FOR-MAINTENANCE-OF-THE-BLOOD-PLACENTAL-BAR. Acesso em: 27/07/2024