ANTIBACTERIAL ACTIVITY EVALUATION OF PHAGICAL ENDOLYSINS PURE AND COMBINED WITH NATURAL COMPONENTS

Hugo Martins Correia; Mateus Pereira Teles; Marco Túlio Pardini Gontijo; Pâmela Talita de Aguiar e Silva; Roberto Gomes de Souza Berlinck; Marcelo Brocchi

Todos os Trabalhos

Trabalho

Título do Trabalho

ANTIBACTERIAL ACTIVITY EVALUATION OF PHAGICAL ENDOLYSINS PURE AND COMBINED WITH NATURAL COMPONENTS

Autores

Hugo Martins Correia
Mateus Pereira Teles
Marco Túlio Pardini Gontijo
Pâmela Talita de Aguiar e Silva
Roberto Gomes de Souza Berlinck
Marcelo Brocchi

Modalidade

Apresentação de Pôster

Área temática

Genética de Microrganismos

Data de Publicação

09/06/2023

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/v-gbmeeting/609738-antibacterial-activity-evaluation-of-phagical-endolysins-pure-and-combined-with-natural-components

ISBN

978-85-5722-785-9

Palavras-Chave

multidrug-resistant bacteria, endolysin, natural compounds, antibacterial activity, antibiotic substitute

Resumo

Some bacterial strains of the ESKAPE group (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter spp.) are drug multiresistant. Endolysins are proteins with potential for clinical use in treatments against resistant bacteria, due to their antimicrobial activity. For its application, one of the barriers to the exogenous use of endolysins against Gram-negative bacteria is the difficulty for crossing the outer membrane. SAR-endolysins are activated by pro-membrane destabilization in the outer membrane. Preliminary studies show that these proteins are able to penetrate the outer membrane. In addition, several compounds have the ability to destabilize both cytoplasmic and outer bacterial membranes, and already shown synergy with endolysins against Gram-positive and Gram-negative bacteria. Thus, the objective of this project is the recombinant expression and purification of a phage LysM SAR-endolysin, in silico analysis, and investigating their antibacterial activity, alone or combined permeabilizing agents, against bacteria of ESKAPE group through in vitro and in vivo assays in the Galleria mellonella model. We are performing expression and protein purification, however we have already analyzed the LysM SAR-endolysin by ProtParam tool which identified a molecular weight of 47263.73 Daltons, has a theoretical half-life higher than 10 hours inside cells like Escherichia coli, instability index of 15.15 classifying this protein as stable; GRAVY index of -0.323 indicating that the protein is hydrophilic. The theoretical isoelectric point is 6.03, calculated using the Isoelectric Point Calculator 2.0 (IPC 2.0) web server. Therefore, we concluded that LysM is stable and hydrophilic.

Título do Evento: V GBMeeting
Cidade do Evento: Campinas
Título dos Anais do Evento: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora: Even3
Meio de Divulgação: Meio Digital
DOI: Obter o DOI

Como citar

CORREIA, Hugo Martins et al.. ANTIBACTERIAL ACTIVITY EVALUATION OF PHAGICAL ENDOLYSINS PURE AND COMBINED WITH NATURAL COMPONENTS.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609738-ANTIBACTERIAL-ACTIVITY-EVALUATION-OF-PHAGICAL-ENDOLYSINS-PURE-AND-COMBINED-WITH-NATURAL-COMPONENTS. Acesso em: 27/07/2024