AN ANALYSIS OF OSCA GENE NETWORKS IN VELLOZIA SPP. UNDER VARIOUS WATER CONDITIONS TO IDENTIFY KEY GENE INTERACTORS INVOLVED IN DROUGHT TOLERANCE STRATEGIES.

Mariana Feitosa Cavalheiro; Felipe Eduardo Ciamponi; Diego Mauricio Riaño-Pachon; Lucas Eduardo Canesin; Paulo Arruda; Ricardo Augusto Dante; Isabel Rodrigues Gerhardt

Título do Trabalho

Autores

Mariana Feitosa Cavalheiro
Felipe Eduardo Ciamponi
Diego Mauricio Riaño-Pachon
Lucas Eduardo Canesin
Paulo Arruda
Ricardo Augusto Dante
Isabel Rodrigues Gerhardt

Modalidade

Apresentação de Pôster

Área temática

Genética Vegetal e Melhoramento

Data de Publicação

09/06/2023

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/v-gbmeeting/609718-an-analysis-of-osca-gene-networks-in-vellozia-spp-under-various-water-conditions-to-identify-key-gene-interactor

ISBN

978-85-5722-785-9

Palavras-Chave

mechanosensitive channels, abiotic stress response, weighted gene co-expression network analysis, wild plants

Resumo

The Brazilian campos rupestres, a biodiversity hotspot, is an ecoregion that frequently experiences prolonged droughts. Plants that inhabit this environment have developed various strategies to deal with water scarcity, particularly those from the Velloziacea family. In order to gain an understanding of the mechanisms involved in these diverse drought-coping strategies, we selected two representative species from the Vellozia genus: the desiccation-tolerant V. nivea and the evergreen V. intermedia. To study the molecular basis of these phenotypes, we focused on the OSCA gene family, which encodes transmembrane proteins known as OSCA channels. These channels play a role in increasing [Ca2+]i levels in response to osmotic stress in plants. Differences in gene expression patterns related to OSCA may indicate that the two species employ distinct drought perception mechanisms. To further investigate this, we collected 80 samples comprising leaf and root tissues from both species and exposed them to four different water regimes: early drought (ED), late drought (LD), early rain (ER), and late rain (LR). We then used weighted gene co-expression network analysis (WGCNA) to generate gene interaction networks from the dataset. By characterizing the modules of interest based on their expression patterns, and identifying the OSCA-connected genes within these modules, we were able to identify functional enrichment of these genes. We found 13 modules (8 for leaf and 5 for root) with an average of 300 genes per module and 4 modules (3 for leaf and 1 for root) with an average of 3200 genes per module that are associated with OSCA expression in V. intermedia and V. nivea, respectively. Furthermore, some of these modules were enriched in functions related to abiotic stress response, such as nitric oxide and reactive oxygen species responsiveness, as well as photosynthetic metabolism in the leaf. Additionally, we observed that the evergreen V. intermedia had a higher degree of modularity compared to V. nivea, indicating a more complex transcriptional regulatory network is required for it to survive under varying water availability. By utilizing cutting-edge gene network modeling, we were able to identify key interactors associated with OSCA genes in the different strategies for coping with drought. Our findings on the transcriptomic landscape of Vellozia spp. genes may provide new insights into how wild plants respond to drought, which is a pressing concern in a world facing the challenges of climate change.

Título do Evento: V GBMeeting
Cidade do Evento: Campinas
Título dos Anais do Evento: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

CAVALHEIRO, Mariana Feitosa et al.. AN ANALYSIS OF OSCA GENE NETWORKS IN VELLOZIA SPP. UNDER VARIOUS WATER CONDITIONS TO IDENTIFY KEY GENE INTERACTORS INVOLVED IN DROUGHT TOLERANCE STRATEGIES... In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609718-AN-ANALYSIS-OF-OSCA-GENE-NETWORKS-IN-VELLOZIA-SPP-UNDER-VARIOUS-WATER-CONDITIONS-TO-IDENTIFY-KEY-GENE-INTERACTOR. Acesso em: 18/04/2025