THE ROLE OF THE INTESTINAL MICROBIOTA IN SUSCEPTIBILITY TO THE DEVELOPMENT OF SAINT LOUIS

Jaqueline de Souza Felipe; Aelxandre Borin; Laís Durço Coimbra; Marina Alves Fontoura; Rafael Elias Marques

Todos os Trabalhos

Trabalho

Título do Trabalho

THE ROLE OF THE INTESTINAL MICROBIOTA IN SUSCEPTIBILITY TO THE DEVELOPMENT OF SAINT LOUIS

Autores

Jaqueline de Souza Felipe
Aelxandre Borin
Laís Durço Coimbra
Marina Alves Fontoura
Rafael Elias Marques

Modalidade

Apresentação de Pôster

Área temática

Imunologia

Data de Publicação

09/06/2023

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/v-gbmeeting/609714-the-role-of-the-intestinal-microbiota-in-susceptibility-to-the-development-of-saint-louis

ISBN

978-85-5722-785-9

Palavras-Chave

Encefalite Saint Louis, SLEV, Microbiota

Resumo

Saint Louis encephalitis virus (SLEV) belongs to the Flavivirus genus of the Flaviviridae family. SLEV is an arbovirus transmitted by mosquitoes of the genus Culex and is associated with developing severe neurological diseases, characterized by meningoencephalitis and a high mortality rate. Although studies have shown the role of the immune system in the antiviral response decreasing viral replication on SLEV infection, the contribution of other aspects of the immune response remains unknown. It is known that the interaction of the immune system with the microbiota affects the systemic immune response. The intestinal microbiota has immunomodulatory functions that have been studied in the context of arboviral infections. However, the role of the microbiota in viral infections that affect the central nervous system, as in the case of St. Louis, is unknown. In this sense, we hypothesize that after depletion of the intestinal microbiota, mice will become more susceptible to SLEV infection, due to impaired development of the immune response. We aim to evaluate the role of intestinal microbiota in the development of disease caused by SLEV in vivo. Thus, we infected C57BL/6 mice treated with a cocktail of antibiotics and an untreated control group infected with SLEV. Our results showed that mice with depleted microbiota and infected with SLEV are more susceptible to the development of the disease with higher mortality rates (83%) than infected animals with intact microbiota (50%). Our data also showed a reduction in the expression of genes and the microbiota bacterial density, confirming the microbiota depletion in the infected animals. The ELISA assay demonstrated that mice with depleted microbiota showed alterations in cytokines and chemokines that are important in the immune response. Our data may show unknown aspects of viral biology. Acknowledgments: We thank FINEP and UNICAMP for financial support and CNPEM for infrastructure support.

Título do Evento: V GBMeeting
Cidade do Evento: Campinas
Título dos Anais do Evento: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora: Even3
Meio de Divulgação: Meio Digital
DOI: Obter o DOI

Como citar

FELIPE, Jaqueline de Souza et al.. THE ROLE OF THE INTESTINAL MICROBIOTA IN SUSCEPTIBILITY TO THE DEVELOPMENT OF SAINT LOUIS.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609714-THE-ROLE-OF-THE-INTESTINAL-MICROBIOTA-IN-SUSCEPTIBILITY-TO-THE-DEVELOPMENT-OF-SAINT-LOUIS. Acesso em: 27/07/2024