SALMONELLA ENTERICA AND OUTER MEMBRANE VESICLES (OMVS) AS PLATFORM FOR VACCINE DEVELOPMENT

Marina Floro e Silva; Marcelo Brocchi

Todos os Trabalhos

Trabalho

Título do Trabalho

SALMONELLA ENTERICA AND OUTER MEMBRANE VESICLES (OMVS) AS PLATFORM FOR VACCINE DEVELOPMENT

Autores

Marina Floro e Silva
Marcelo Brocchi

Modalidade

Apresentação de Pôster

Área temática

Microbiologia

Data de Publicação

09/06/2023

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/v-gbmeeting/609689-salmonella-enterica-and-outer-membrane-vesicles-(omvs)-as-platform-for-vaccine-development

ISBN

978-85-5722-785-9

Palavras-Chave

Outer Membrane Vesicles, Vaccine, Salmonella enterica

Resumo

Salmonella enterica is an important pathogen for humans and other animals causing since enteric to severe systemic infections. After oral ingestion, S. enterica can bind, proliferate, and invade the intestinal mucosa cells. Depending on the bacterial serovar and the host susceptibility, the infection can remain localized to the intestinal subepithelial mucosa or can progress to a systemic infection. However, S. enterica virulence can be attenuated by the deletion of specific target genes. The attenuated strains are good carriers of antigens, DNA and even RNA-based vaccines been able to carry and present macromolecules to cells of the immune system. In addition, S. enterica can be modified to produce a hypervesiculation phenotype (high level of OMVs production). Moreover, despite many efforts, few live recombinant attenuated strains are in used nowadays manly due to the challenge in developing strains with appropriate balance between attenuation and immunogenicity. Therefore, the aim of this study is the development of new attenuated strains targeting the deletion of DNA-binding proteins encoding genes with important functions in chromosome structures and DNA topology. Additionally, we will explore the induced attenuation phenotype in which the recombinant strains exhibit a progressive attenuation profile after host infection. We will also explore the use OMVs as carrier of DNA and RNA-based vaccines. For this, mutant strains will be constructed exhibiting the hypervesiculating phenotype and a detoxified LPS. At the end of this study, we hope to contribute with the development an innovative platform for vaccine development based on the biotechnological potential of S. enterica.

Título do Evento: V GBMeeting
Cidade do Evento: Campinas
Título dos Anais do Evento: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

SILVA, Marina Floro e; BROCCHI, Marcelo. SALMONELLA ENTERICA AND OUTER MEMBRANE VESICLES (OMVS) AS PLATFORM FOR VACCINE DEVELOPMENT.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609689-SALMONELLA-ENTERICA-AND-OUTER-MEMBRANE-VESICLES-(OMVS)-AS-PLATFORM-FOR-VACCINE-DEVELOPMENT. Acesso em: 05/10/2024