CROSSTALK BETWEEN MICROBIOTA AND HISTONES MODIFICATION IN THE HEALTHY GUT

Publicado em 09/06/2023 - ISBN: 978-85-5722-785-9

Título do Trabalho
CROSSTALK BETWEEN MICROBIOTA AND HISTONES MODIFICATION IN THE HEALTHY GUT
Autores
  • Dieggo Rodrigues de Paula
  • Prof. Dr. Marco Aurélio Vinolo
  • Patrick Varga Weisz
Modalidade
Apresentação de Pôster
Área temática
Imunologia
Data de Publicação
09/06/2023
País da Publicação
Brasil
Idioma da Publicação
Inglês
Página do Trabalho
https://www.even3.com.br/anais/v-gbmeeting/609665-crosstalk-between-microbiota-and-histones-modification-in-the-healthy-gut
ISBN
978-85-5722-785-9
Palavras-Chave
colon, chromatin, histones, intestines, crotonylation, microbiota
Resumo
Introduction: The gut, especially the colon, is home of an ecosystem of microbes that are important for digestion and for training of the immune system. The microbiota influences gene expression in the intestinal epithelium and this is important for the normal metabolism of epithelial cells and innate immunity. One way by which the microbiota affect gut physiology and gene expression is by generating short-chain fatty acids that, in turn, influence histone post-translational modifications [1]. In this study, we show that global levels of many histone crotonylation marks are responsive to depletion of the microbiota and highlight the core effect associated with the microbiota via comparison of germ-free and antibiotic-treated animals. Methods and Results: Male C57BL/6 at age 8–12 weeks was treated with 200?µl of a mixture of antibiotics cocktail daily for 3 days by gavage. Colon epithelium was extracted using EDTA, followed by enzymatic dispersion of cells [2]. Chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) was performed as described previously [2]. Anti-Crotonyllysine antibody was used to tag lysine crotonylation. For population mRNA sequencing (mRNA-seq), cDNA libraries for sequencing were generated using standard non-diretional kit for Illumina®. Sequencing was done by Novagen company. Bioinformatic analysis of ChIP-seq and mRNA-seq was done using SeqMonk and R packages. Raw sequenced RNA-seq data from germ-free vs conventional animals were obtained from published data [3]. In animals treated with antibiotics, 248 genes were upregulated and 137 were downregulated (Fold change 1.5). About 44% and 48% of these genes, respectively, overlapped with up- and down-regulated genes in germ-free animals compared to conventionally reared mice. Upregulated and overlapping genes return GO terms of immune response regulation and T cell activation and differentiation. There was a general decrease in the lysine crotonylation of histones from the antibiotic-treated animals, however, the relative proportion of this modification in the promoter regions of genes (<=1kb) increased. We saw a significant overlap of genes that show changes in expression and changes in crotonyation dependent on the microbiota. Conclusion: Histone crotonylation changes reflect microbiota-dependent gene expression of a subset of genes. Research Supported by FAPESP References 1. Mol Metab. 38:100925, 2020; 2. Nat Commun. 9:105, 2018; 3. Nat Microbiol. 5:610-619, 2020.
Título do Evento
V GBMeeting
Cidade do Evento
Campinas
Título dos Anais do Evento
Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora
Even3
Meio de Divulgação
Meio Digital

Como citar

PAULA, Dieggo Rodrigues de; VINOLO, Prof. Dr. Marco Aurélio; WEISZ, Patrick Varga. CROSSTALK BETWEEN MICROBIOTA AND HISTONES MODIFICATION IN THE HEALTHY GUT.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609665-CROSSTALK-BETWEEN-MICROBIOTA-AND-HISTONES-MODIFICATION-IN-THE-HEALTHY-GUT. Acesso em: 13/11/2024

Trabalho

Even3 Publicacoes