GENOMIC-BASED SECONDARY METABOLISM CHARACTERIZATION OF TRICHODERMA HARZIANUM, TRICHODERMA ATROVIRIDE, AND TRICHODERMA REESEI

Publicado em 09/06/2023 - ISBN: 978-85-5722-785-9

Título do Trabalho
GENOMIC-BASED SECONDARY METABOLISM CHARACTERIZATION OF TRICHODERMA HARZIANUM, TRICHODERMA ATROVIRIDE, AND TRICHODERMA REESEI
Autores
  • Paulo Henrique Campiteli de Azevedo
  • Rafaela Rossi Rosolen
  • Maria Augusta Crivelente Horta
  • Anete Pereira de Souza
Modalidade
Apresentação de Pôster
Área temática
Genética de Microrganismos
Data de Publicação
09/06/2023
País da Publicação
Brasil
Idioma da Publicação
Inglês
Página do Trabalho
https://www.even3.com.br/anais/v-gbmeeting/609656-genomic-based-secondary-metabolism-characterization-of-trichoderma-harzianum-trichoderma-atroviride-and-trichod
ISBN
978-85-5722-785-9
Palavras-Chave
Secondary metabolism, Genomics, pacbio
Resumo
Trichoderma (Ascomycetes) is a generalist opportunistic genus, with elevated biotechnological potential. Collectively, they display a diverse set of bioactive enzymes and natural compounds with strong biodegradative activities able to efficiently degrade complex biomass, antibiotic and antimycotic activities, beneficial interaction with plants and many more hidden features. Currently, T. reesei QM6a is the major model for ligninolytic enzyme production on industrial levels, while other species are considered emerging biocontrol agents with expanding markets worldwide. Despite major biotechnological relevance, Trichoderma-based studies face the unexplored state of wild Trichoderma strains and inactive/cryptic metabolites preventing their bioprospection. Natural compounds are bioactive enzymes originating from a biosynthetic backbone enzyme, Polyketides Synthases (PKS), Non-Ribosomal-Polyketide-Synthases (NRPS), Hybrid PKS/NRPS, and Terpene Cyclases (TC), flanked by transporters, editing genes and in some cases resistance genes. Such gene tends to be organized on colocalized gene clusters and act in synergy to respond to specific environmental cues. This study subjected four newly sequenced Pacbio genomes, T. harzianum 179, T. harzianum 3844, T. reesei 711, and T. atroviride 20, to multiple bioinformatic tools aiming for genomic characterization, followed by antiSMASH to identify secondary metabolite gene clusters. It was possible to recover major specialized metabolism regions, compare micro-synteny and collinearity of secondary metabolites gene clusters, and inspect cluster activation triggered by glucose and cellulosic substrates. Such result offer subsidies for future experimental analysis aiming at silent cluster activation, paving the way for the compound discovery of wild Trichoderma ssp. and a broader understanding of regulatory control of secondary metabolites production.
Título do Evento
V GBMeeting
Cidade do Evento
Campinas
Título dos Anais do Evento
Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora
Even3
Meio de Divulgação
Meio Digital
DOI
LinkObter o DOI

Como citar

AZEVEDO, Paulo Henrique Campiteli de et al.. GENOMIC-BASED SECONDARY METABOLISM CHARACTERIZATION OF TRICHODERMA HARZIANUM, TRICHODERMA ATROVIRIDE, AND TRICHODERMA REESEI.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609656-GENOMIC-BASED-SECONDARY-METABOLISM-CHARACTERIZATION-OF-TRICHODERMA-HARZIANUM-TRICHODERMA-ATROVIRIDE-AND-TRICHOD. Acesso em: 24/06/2024

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