GENOMIC-BASED SECONDARY METABOLISM CHARACTERIZATION OF TRICHODERMA HARZIANUM, TRICHODERMA ATROVIRIDE, AND TRICHODERMA REESEI

Paulo Henrique Campiteli de Azevedo; Rafaela Rossi Rosolen; Maria Augusta Crivelente Horta; Anete Pereira de Souza

Título do Trabalho

Autores

Paulo Henrique Campiteli de Azevedo
Rafaela Rossi Rosolen
Maria Augusta Crivelente Horta
Anete Pereira de Souza

Modalidade

Apresentação de Pôster

Área temática

Genética de Microrganismos

Data de Publicação

09/06/2023

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/v-gbmeeting/609656-genomic-based-secondary-metabolism-characterization-of-trichoderma-harzianum-trichoderma-atroviride-and-trichod

ISBN

978-85-5722-785-9

Palavras-Chave

Secondary metabolism, Genomics, pacbio

Resumo

Trichoderma (Ascomycetes) is a generalist opportunistic genus, with elevated biotechnological potential. Collectively, they display a diverse set of bioactive enzymes and natural compounds with strong biodegradative activities able to efficiently degrade complex biomass, antibiotic and antimycotic activities, beneficial interaction with plants and many more hidden features. Currently, T. reesei QM6a is the major model for ligninolytic enzyme production on industrial levels, while other species are considered emerging biocontrol agents with expanding markets worldwide. Despite major biotechnological relevance, Trichoderma-based studies face the unexplored state of wild Trichoderma strains and inactive/cryptic metabolites preventing their bioprospection. Natural compounds are bioactive enzymes originating from a biosynthetic backbone enzyme, Polyketides Synthases (PKS), Non-Ribosomal-Polyketide-Synthases (NRPS), Hybrid PKS/NRPS, and Terpene Cyclases (TC), flanked by transporters, editing genes and in some cases resistance genes. Such gene tends to be organized on colocalized gene clusters and act in synergy to respond to specific environmental cues. This study subjected four newly sequenced Pacbio genomes, T. harzianum 179, T. harzianum 3844, T. reesei 711, and T. atroviride 20, to multiple bioinformatic tools aiming for genomic characterization, followed by antiSMASH to identify secondary metabolite gene clusters. It was possible to recover major specialized metabolism regions, compare micro-synteny and collinearity of secondary metabolites gene clusters, and inspect cluster activation triggered by glucose and cellulosic substrates. Such result offer subsidies for future experimental analysis aiming at silent cluster activation, paving the way for the compound discovery of wild Trichoderma ssp. and a broader understanding of regulatory control of secondary metabolites production.

Título do Evento: V GBMeeting
Cidade do Evento: Campinas
Título dos Anais do Evento: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

AZEVEDO, Paulo Henrique Campiteli de et al.. GENOMIC-BASED SECONDARY METABOLISM CHARACTERIZATION OF TRICHODERMA HARZIANUM, TRICHODERMA ATROVIRIDE, AND TRICHODERMA REESEI.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609656-GENOMIC-BASED-SECONDARY-METABOLISM-CHARACTERIZATION-OF-TRICHODERMA-HARZIANUM-TRICHODERMA-ATROVIRIDE-AND-TRICHOD. Acesso em: 10/02/2025