PATHWAY IMPROVEMENT FOR ETHANOL PRODUCTION IN THERMOPHILIC BACTERIA USING PYRUVATE DECARBOXYLASE

Milena Andreotti Minetto; Daniel Groban Olson

Todos os Trabalhos

Trabalho

Título do Trabalho

PATHWAY IMPROVEMENT FOR ETHANOL PRODUCTION IN THERMOPHILIC BACTERIA USING PYRUVATE DECARBOXYLASE

Autores

Milena Andreotti Minetto
Daniel Groban Olson

Modalidade

Apresentação de Pôster

Área temática

Genética de Microrganismos

Data de Publicação

09/06/2023

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/v-gbmeeting/609648-pathway-improvement-for-ethanol-production-in-thermophilic-bacteria-using-pyruvate-decarboxylase

ISBN

978-85-5722-785-9

Palavras-Chave

Ethanol, metabolic pathway, pyruvate decarbolylase (PDC), alcohol dehydrogenase (ADH), formate dehydrogenase (FDH)

Resumo

To produce ethanol, Clostridium thermocellum, a thermophilic, anaerobic bacteria, uses a complex and multiplex metabolic pathway that uses the pyruvate ferredoxin oxidoreductase (PFOR) enzyme. However, the ethanol titer produced by this organism is too low for commercial applications. Most commercial ethanol production relies on a much simpler metabolic pathway that uses the pyruvate decarboxylase (PDC) enzyme, which is found in both bacteria and yeast (e.g. Saccharomyces cerevisiae), but does not function at thermophilic temperatures. Previous attempts to introduce PDC activity into thermophilic bacteria, such as C. thermocellum, have not been successful. The aimed pathway involves PDC and alcohol dehydrogenase (ADH) to convert pyruvate to acetaldehyde and then ethanol. This pathway also converts NADH to NAD, so for in-vitro reactions, we often include a third enzyme, formate dehydrogenase (FDH) to recycle the resulting NAD back to NADH. Aiming to build a computational model that reflects the actual pathway in E. coli and help troubleshoot the C. thermocellum cell free system to produce high ethanol titer, my goal is to demonstrate that the in-vitro PDC-ADH-FDH system is capable of producing high concentrations of ethanol (> 1 M) and determine the extent to which this behavior can be mathematically modeled using existing kinetic models.

Título do Evento: V GBMeeting
Cidade do Evento: Campinas
Título dos Anais do Evento: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

MINETTO, Milena Andreotti; OLSON, Daniel Groban. PATHWAY IMPROVEMENT FOR ETHANOL PRODUCTION IN THERMOPHILIC BACTERIA USING PYRUVATE DECARBOXYLASE.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609648-PATHWAY-IMPROVEMENT-FOR-ETHANOL-PRODUCTION-IN-THERMOPHILIC-BACTERIA-USING-PYRUVATE-DECARBOXYLASE. Acesso em: 03/10/2024