GAMMA-DELTA T CELLS AND IL-22 PATHWAY ARE ASSOCIATED TO CELL PROLIFERATION INDUCED BY INULIN DIET

Helder Carvalho De Assis; Lais Passariello Pral; SARAH DE OLIVEIRA; José Luís Fachi; Arilson B. S. P. Gomes; VALQUIRIA APARECIDA MATHEUS; Prof. Dr. Marco Aurélio Vinolo

Todos os Trabalhos

Trabalho

Título do Trabalho

GAMMA-DELTA T CELLS AND IL-22 PATHWAY ARE ASSOCIATED TO CELL PROLIFERATION INDUCED BY INULIN DIET

Autores

Helder Carvalho De Assis
Lais Passariello Pral
SARAH DE OLIVEIRA
José Luís Fachi
Arilson B. S. P. Gomes
VALQUIRIA APARECIDA MATHEUS
Prof. Dr. Marco Aurélio Vinolo

Modalidade

Apresentação de Pôster

Área temática

Imunologia

Data de Publicação

09/06/2023

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/v-gbmeeting/609626-gamma-delta-t-cells-and-il-22-pathway-are-associated-to-cell-proliferation-induced-by-inulin-diet

ISBN

978-85-5722-785-9

Palavras-Chave

Intraepithelial lymphocytes, epithelium, inulin

Resumo

Abstract: Fermentation of inulin fiber is known to induce IL-22 production, enhancing barrier function and epithelial regeneration. Gamma-delta T cells in the gut produce IL-22 and interact directly with IECs through the Myd88 axis. Furthermore, gamma-delta T cells can sense nutrients from the diet, reprogramming their activity depending on the diet composition. Although the inulin diet has shown effects on immunity and the gut, few studies showed how gamma-delta T cells sense and react to inulin diet and inulin-enriched microbiota. In this work, we intend to clarify how gamma-delta T cells respond to the inulin-induced microenvironment and its role in proliferative phenotype by ISCs. Methods: C57BL/6 mice wild-type and deficient for gamma-delta T cells (TCRd-/-), IL-22 (IL-22-/-) and aryl hydrocarbon receptor (AHR-/-) were fed with inulin-supplemented diet (AIN93 + 10% inulin) for 30 days. After the diet period, the mice were euthanized and several parameters were evaluated to confirm the proliferative effect provoked by inulin: size of colon, caecum, crypt length and EdU+ cells in crypts. Subpopulations of lymphocytes of intestinal epithelium and lamina propria were identified by flow cytometry. Gamma-delta T cells from small intestine were sorted and its RNA were sequenced for differentially expressed genes analysis. Results: Gamma-delta T cells from inulin-fed mice presented several genes differentially expressed compared to controls. A downregulation of inflammatory genes and upregulation of pathways associated with metabolism and HIF-1 were prominent on this analysis. TCRd-/-, IL-22-/- and AHR-/- mice fed with inulin did not show the proliferative phenotype induced by inulin, suggesting that the IL-22 axis, activation of AHR and activity of gamma-delta T cells may be crucial for it, but more information is needed to understand with these factor are connected or not. Furthermore, we observed that mice that were fed with inulin diet and had it replaced with a control diet for 1 and 2 weeks presented the partial reversion of the phenotype, indicating that the sensing of inulin nutrients is also important to maintain the proliferation. Finally, the findings may provide new insights into therapeutic utilizations for inulin in the maintenance of gut homeostasis. Financial support: FAPESP (process 2020/00311-0)

Título do Evento: V GBMeeting
Cidade do Evento: Campinas
Título dos Anais do Evento: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora: Even3
Meio de Divulgação: Meio Digital
DOI: Obter o DOI

Como citar

ASSIS, Helder Carvalho De et al.. GAMMA-DELTA T CELLS AND IL-22 PATHWAY ARE ASSOCIATED TO CELL PROLIFERATION INDUCED BY INULIN DIET.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609626-GAMMA-DELTA-T-CELLS-AND-IL-22-PATHWAY-ARE-ASSOCIATED-TO-CELL-PROLIFERATION-INDUCED-BY-INULIN-DIET. Acesso em: 27/07/2024