ELUCIDATING MOBILIZED COLISTIN RESISTANCE GENES RELATIONSHIPS ON SALMONELLA ENTERICA

Maria Luiza Andreani; Marcelo Brocchi; Juliana José

Todos os Trabalhos

Trabalho

Título do Trabalho

ELUCIDATING MOBILIZED COLISTIN RESISTANCE GENES RELATIONSHIPS ON SALMONELLA ENTERICA

Autores

Maria Luiza Andreani
Marcelo Brocchi
Juliana José

Modalidade

Apresentação Oral

Área temática

Genética de Microrganismos

Data de Publicação

09/06/2023

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/v-gbmeeting/609596-elucidating-mobilized-colistin-resistance-genes-relationships-on-salmonella-enterica

ISBN

978-85-5722-785-9

Palavras-Chave

Salmonella, Antibiotic resistance, pangenome

Resumo

Salmonella enterica is a gram-negative bacterium that can infect humans and animals used in the food industry. S. enterica has an important role in the problem of emerging antibiotic resistance. Bad administration of antibiotics has pushed the use of last-resort antibiotics, such as colistin. Yet, the continued use of colistin in animal husbandry has led to the emergence of mobilized colistin resistance genes (MCRs). In light of that, curated databases enable the study of the evolutionary events and genomic context of elusive genes such as the mcr, which are mostly found on plasmids. Here, we provide a pangenome analysis of 1544 Salmonella enterica genomes from a curated database, including mcr-positive and mcr-negative samples. We found a prevalence of mcr-9, followed by mcr-1 among the samples. Further analysis of genomic distances revealed a tendency for accessory distances among mcr-positive samples to be smaller than between mcr-positive and negative samples, suggesting a more similar composition. Moreover, we analyze the pattern of association and dissociation between genes in the pangenome, and after inferring the location of the genes that associate and dissociate with mcr genes, a pattern of association linked to plasmids and dissociation linked to chromosomes emerged. Further, functional evaluation of mcr-associated and dissociated genes revealed that viral proteins could take part in both association and dissociation processes, which could be pointing to different mobile elements carrying mcr genes. Also, we screened the IGRs related to mcr genes and found already-known IS sequences related to them. These findings point to a dynamic in the genetic context of mcr genes influenced by horizontal transmission. Moreover, it sheds light on the antagonistic relationship between the two more common mcr genes on the dataset, mcr-1 and mcr-9 on Salmonella enterica. Fomento: CAPES

Título do Evento: V GBMeeting
Cidade do Evento: Campinas
Título dos Anais do Evento: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

ANDREANI, Maria Luiza; BROCCHI, Marcelo; JOSÉ, Juliana. ELUCIDATING MOBILIZED COLISTIN RESISTANCE GENES RELATIONSHIPS ON SALMONELLA ENTERICA.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609596-ELUCIDATING-MOBILIZED-COLISTIN-RESISTANCE-GENES-RELATIONSHIPS-ON-SALMONELLA-ENTERICA. Acesso em: 15/05/2025