DYNAMIC CHANGES IN HISTONE ACETYLATION AND CROTONYLATION DURING INTESTINAL INFLAMMATION

Publicado em 09/06/2023 - ISBN: 978-85-5722-785-9

Título do Trabalho
DYNAMIC CHANGES IN HISTONE ACETYLATION AND CROTONYLATION DURING INTESTINAL INFLAMMATION
Autores
  • Mariane Font Fernandes
  • MARIANA PORTOVEDO MARTINI
  • SARAH DE OLIVEIRA
  • Arilson B. S. P. Gomes
  • VALQUIRIA APARECIDA MATHEUS
  • Prof. Dr. Marco Aurélio Vinolo
Modalidade
Apresentação de Pôster
Área temática
Imunologia
Data de Publicação
09/06/2023
País da Publicação
Brasil
Idioma da Publicação
Inglês
Página do Trabalho
https://www.even3.com.br/anais/v-gbmeeting/609572-dynamic-changes-in-histone-acetylation-and-crotonylation-during-intestinal-inflammation
ISBN
978-85-5722-785-9
Palavras-Chave
Histone acetylation, Histone crotonylation, Intestinal inflammation, Intestinal epithelial cells
Resumo
INTRODUCTION: The intestinal epithelial cells constitute the first physical barrier of the gastrointestinal tract to components of the intestinal microbiota. This interaction may modulate gene expression via histone post-translational modifications (HPTMs), including acetylation and crotonylation, which promote an active transcriptional profile. Aberrant regulations of HPTMs have been associated with different diseases in this environment, such as inflammatory bowel disease. However, little is known about their role in the development of intestinal inflammation and factors that influence them. METHODS AND RESULTS: In our study, we analyzed six HPTMs (H3K18ac, H3K18cr, H4K8ac, H4K8cr, Kac and Kcr) in epithelial cell obtained from animals treated with 2.5% dextran sulfate sodium (DSS, colitis model) and observed an increase in the levels of all on the third day after DSS administration. This treatment time is considered the beginning of inflammation, with no significant difference in the structure of the crypts, the amount of inflammatory infiltrate and the concentration of cytokines compared to the control condition. On the seventh day, corresponding to the acute phase with exacerbated inflammation and a drastic reduction in the colonic concentration of short-chain fatty acids (SCFAs), there is a decrease in H3K18cr and H4K8ac, while the other HPTMs have similar levels to the control. CONCLUSION: These findings indicate that HPTMs may be important in the changes that occur before and after the establishment of inflammation in the colon. The causes and consequences of this dynamic pattern of histone acetylation and crotonylation in epithelial cells are still being explored. FOOTNOTE: CAPES (88887.644541/2021-00) and FAPESP (2021/10478-1 and 2018/15313-8)
Título do Evento
V GBMeeting
Cidade do Evento
Campinas
Título dos Anais do Evento
Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora
Even3
Meio de Divulgação
Meio Digital
DOI
LinkObter o DOI

Como citar

FERNANDES, Mariane Font et al.. DYNAMIC CHANGES IN HISTONE ACETYLATION AND CROTONYLATION DURING INTESTINAL INFLAMMATION.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609572-DYNAMIC-CHANGES-IN-HISTONE-ACETYLATION-AND-CROTONYLATION-DURING-INTESTINAL-INFLAMMATION. Acesso em: 20/06/2024

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