DYNAMIC CHANGES IN HISTONE ACETYLATION AND CROTONYLATION DURING INTESTINAL INFLAMMATION

Mariane Font Fernandes; MARIANA PORTOVEDO MARTINI; SARAH DE OLIVEIRA; Arilson B. S. P. Gomes; VALQUIRIA APARECIDA MATHEUS; Prof. Dr. Marco Aurélio Vinolo

Todos os Trabalhos

Trabalho

Título do Trabalho

DYNAMIC CHANGES IN HISTONE ACETYLATION AND CROTONYLATION DURING INTESTINAL INFLAMMATION

Autores

Mariane Font Fernandes
MARIANA PORTOVEDO MARTINI
SARAH DE OLIVEIRA
Arilson B. S. P. Gomes
VALQUIRIA APARECIDA MATHEUS
Prof. Dr. Marco Aurélio Vinolo

Modalidade

Apresentação de Pôster

Área temática

Imunologia

Data de Publicação

09/06/2023

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/v-gbmeeting/609572-dynamic-changes-in-histone-acetylation-and-crotonylation-during-intestinal-inflammation

ISBN

978-85-5722-785-9

Palavras-Chave

Histone acetylation, Histone crotonylation, Intestinal inflammation, Intestinal epithelial cells

Resumo

INTRODUCTION: The intestinal epithelial cells constitute the first physical barrier of the gastrointestinal tract to components of the intestinal microbiota. This interaction may modulate gene expression via histone post-translational modifications (HPTMs), including acetylation and crotonylation, which promote an active transcriptional profile. Aberrant regulations of HPTMs have been associated with different diseases in this environment, such as inflammatory bowel disease. However, little is known about their role in the development of intestinal inflammation and factors that influence them. METHODS AND RESULTS: In our study, we analyzed six HPTMs (H3K18ac, H3K18cr, H4K8ac, H4K8cr, Kac and Kcr) in epithelial cell obtained from animals treated with 2.5% dextran sulfate sodium (DSS, colitis model) and observed an increase in the levels of all on the third day after DSS administration. This treatment time is considered the beginning of inflammation, with no significant difference in the structure of the crypts, the amount of inflammatory infiltrate and the concentration of cytokines compared to the control condition. On the seventh day, corresponding to the acute phase with exacerbated inflammation and a drastic reduction in the colonic concentration of short-chain fatty acids (SCFAs), there is a decrease in H3K18cr and H4K8ac, while the other HPTMs have similar levels to the control. CONCLUSION: These findings indicate that HPTMs may be important in the changes that occur before and after the establishment of inflammation in the colon. The causes and consequences of this dynamic pattern of histone acetylation and crotonylation in epithelial cells are still being explored. FOOTNOTE: CAPES (88887.644541/2021-00) and FAPESP (2021/10478-1 and 2018/15313-8)

Título do Evento: V GBMeeting
Cidade do Evento: Campinas
Título dos Anais do Evento: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

FERNANDES, Mariane Font et al.. DYNAMIC CHANGES IN HISTONE ACETYLATION AND CROTONYLATION DURING INTESTINAL INFLAMMATION.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609572-DYNAMIC-CHANGES-IN-HISTONE-ACETYLATION-AND-CROTONYLATION-DURING-INTESTINAL-INFLAMMATION. Acesso em: 19/04/2025