METABOLOMICS OF PHYTOPHTHORA PARASITICA UNDER DIFFERENT STEROL CONCENTRATIONS: EVALUATING STEROL DEPLETION IN OOMYCETES

Pâmela Ponce Martins; EVANDRO APARECIDO DA SILVA; Flávia Rodrigues Alves Patrício; Taícia Pacheco Fill; Jorge Maurício Costa Mondego

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Trabalho

Título do Trabalho

METABOLOMICS OF PHYTOPHTHORA PARASITICA UNDER DIFFERENT STEROL CONCENTRATIONS: EVALUATING STEROL DEPLETION IN OOMYCETES

Autores

Pâmela Ponce Martins
EVANDRO APARECIDO DA SILVA
Flávia Rodrigues Alves Patrício
Taícia Pacheco Fill
Jorge Maurício Costa Mondego

Modalidade

Apresentação de Pôster

Área temática

Microbiologia

Data de Publicação

09/06/2023

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/v-gbmeeting/609536-metabolomics-of-phytophthora-parasitica-under-different-sterol-concentrations--evaluating-sterol-depletion-in-oom

ISBN

978-85-5722-785-9

Palavras-Chave

phytophthora, esterol, elicitinas, metabolômica

Resumo

Several species of the genus Phytophthora have been described as disease-causing agents in economically important plants in Brazil, for example: in citrus, the species P. parasitica Dastur and P. citrophthora cause gummosis. Belonging to the group of oomycetes, consisting of eukaryotic microorganisms similar to filamentous fungi, Phytophthora spp are hemibiotrophic pathogens. Another characteristic of these pathogens is that they do not synthesize sterol and, therefore, need to capture exogenous sterols from the environment for their normal life cycle. On media lacking sterols, Phytophthora growth tends to be purely vegetative. However, in media to which certain sterols are added, vegetative growth is enhanced and, more significantly, sexual organs (oogonia and antheridia) develop and oospores are formed. However, these microorganisms can synthesize substances necessary for sexual reproduction without the presence of sterol, but require a stress factor to trigger this process. Interestingly, some genes in the sterol synthesis pathway are still present in their genomes. The elicitins, small proteins excreted by Phytophthora spp., may represent a strategy for the pathogen to capture these compounds from plant cell membranes, as they bind to sterol and transfer it between phospholipid membranes. These extracellular proteins are considered PAMPs and are capable of eliciting plant defense reactions. Considering the need for sterol for the development of the pathogen, understanding the uptake mechanism of this compound, how the oomycete uses it and how it responds to its absence in the environment, is essential to formulate strategies to combat the pathogen. One of the studies capable of showing how this microorganism uses sterol is through metabolomics, which aims to generate a global profile of various biochemical metabolites of a biological system, qualitatively and quantitatively, reflecting the activity of its metabolic network. The metabolite profile represents a tool that finds common application in all aspects of discovery, understanding and utilization and therefore represents an important point in studies of physiology and taxonomy. Linking gene function to specific metabolic capabilities or metabolite production is crucial for understanding and developing biotechnological processes. Therefore, the objective of this work will be to analyze the metabolites produced by Phytophthora parasitica in culture medium with different concentrations of ß-sitosterol.

Título do Evento: V GBMeeting
Cidade do Evento: Campinas
Título dos Anais do Evento: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

MARTINS, Pâmela Ponce et al.. METABOLOMICS OF PHYTOPHTHORA PARASITICA UNDER DIFFERENT STEROL CONCENTRATIONS: EVALUATING STEROL DEPLETION IN OOMYCETES.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609536-METABOLOMICS-OF-PHYTOPHTHORA-PARASITICA-UNDER-DIFFERENT-STEROL-CONCENTRATIONS--EVALUATING-STEROL-DEPLETION-IN-OOM. Acesso em: 20/05/2025