REGULATION OF STRESS RESPONSE MEDIATED BY LYSINE CATABOLISM

Publicado em 09/06/2023 - ISBN: 978-85-5722-785-9

Título do Trabalho
REGULATION OF STRESS RESPONSE MEDIATED BY LYSINE CATABOLISM
Autores
  • Ana Lúcia Mendes Pinheiro
  • Viviane Cristina Heinzen da Silva
  • Alessandra Koltun
  • Maísa de Siqueira Pinto
  • Maria Helena Faustinoni Bruno
  • Priscila Zonzini Ramos
  • Juliana Erika de Carvalho Teixeira Yassitepe
  • Paulo Arruda
Modalidade
Apresentação de Pôster
Área temática
Genética Vegetal e Melhoramento
Data de Publicação
09/06/2023
País da Publicação
Brasil
Idioma da Publicação
Inglês
Página do Trabalho
https://www.even3.com.br/anais/v-gbmeeting/609516-regulation-of-stress-response-mediated-by-lysine-catabolism
ISBN
978-85-5722-785-9
Palavras-Chave
lysine catabolism, abiotic stress, biotic stress
Resumo
Lysine catabolism is involved in the stress responses of plants, animals, and microorganisms .The saccharopine pathway (SacPath) is the main route of lysine catabolism in eucaryotes. The first two reactions of SacPath are catalyzed by lysine-ketoglutarate reductase (LKR) and saccharopine dehydrogenase (SDH), domains of a bifunctional polypeptide. LKR converts lysine into saccharopine that is than converted into glutamate (Glu) and aminoadipic semialdehyde (AASA) by SDH. AASA is further converted into aminoadipic acid (AAA) by aminoadipic semialdehyde dehydrogenase (AASADH). In plants, the SacPath is induced by abiotic stress and has been associated with overaccumulation of the osmolytes pipecolate and proline. In addition, the induction of AASADH, the third enzyme of the SacPath, is associated with aldehydes detoxification. In prokaryotes, besides the saccharopine pathway, the conversion of lysine to AASA is also performed by lysine dehydrogenase (LYSDH) that directly converts lysine into AASA. In fact, LYSDH, is hundred times more induced by abiotic stress than the SacPath LKR/SDH enzyme. Besides the saccharopine and the LYSDH pathways being associated with abiotic stress by the overproduction of pipecolate, this intermediary can also be a substrate for the production of N-hydroxy-pipecolic acid (NHP) that is associated with biotic stress in plants. This work aims to unravel the regulation of SacPath and LYSDH pathways in stress responses in plants. We are exploring the potential connections between enzyme kinetics, thermostability osmolites accumulation, and immune signaling to abiotic and biotic stress response. LYSDH genes from the extremophile bacteria Silicibacter pomeroyi and Geobacillus stearothermophillus, previously found to be induced by stress, were used to assemble constructs for overexpression in plants. Transgenic plants overexpressing LYSDHs are being selected and will be phenotyped for abiotic and biotic stress. To inspect the properties of these lysine catabolic enzymes, recombinant LYSDH from extremophiles and SDH from plants were produced in E. coli BL21(DE3)-R3-pRARE2. The recombinant proteins are being characterized for kinetic properties, thermostability, possible inhibition by SacPath intermediaries, and substrate specificity. To get insights into the SacPath participation in abiotic and biotic stress response, maize and Arabidopsis null mutants for LKR/SDH enzyme are being investigated for metabolite accumulation and biotic and abiotic stress response. We expect to contribute to the understanding of lysine catabolism in stress response in plants looking to develop crops resilient to the challenges imposed by climate changes.
Título do Evento
V GBMeeting
Cidade do Evento
Campinas
Título dos Anais do Evento
Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora
Even3
Meio de Divulgação
Meio Digital

Como citar

PINHEIRO, Ana Lúcia Mendes et al.. REGULATION OF STRESS RESPONSE MEDIATED BY LYSINE CATABOLISM.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609516-REGULATION-OF-STRESS-RESPONSE-MEDIATED-BY-LYSINE-CATABOLISM. Acesso em: 05/11/2024

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