MULTI-OMIC APPROACH INDICATES THAT BACTERIAL VOLATILE ORGANIC COMPOUNDS PROMOTE SETARIA VIRIDIS GROWTH MAINLY VIA ENERGY METABOLISM, HORMONAL SIGNALING AND CELL WALL MODULATION

Octávio Augusto Costa Almeida; Bruno Henrique Silva Dias; Natália Oliveira de Araujo; Gabriela Persinoti; Lucas Ferreira Maciel; Celio Cabral Oliveira; Juliana Aparecida Aricetii; Maurício Luís Sforça; Maria Juliana Calderan Rodrigues; Juliana Velasco de Castro Oliveira

Título do Trabalho

Autores

Octávio Augusto Costa Almeida
Bruno Henrique Silva Dias
Natália Oliveira de Araujo
Gabriela Persinoti
Lucas Ferreira Maciel
Celio Cabral Oliveira
Juliana Aparecida Aricetii
Maurício Luís Sforça
Maria Juliana Calderan Rodrigues
Juliana Velasco de Castro Oliveira

Modalidade

Apresentação de Pôster

Área temática

Genética de Microrganismos

Data de Publicação

09/06/2023

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/v-gbmeeting/609265-multi-omic-approach-indicates-that-bacterial-volatile-organic-compounds-promote-setaria-viridis-growth-mainly-via

ISBN

978-85-5722-785-9

Palavras-Chave

volatile organic compounds, plant growth promotion, multi-omics, sustainability

Resumo

Some plant growth promoting bacteria (PGPB) emit volatile organic compounds (VOCs), which are small gaseous signaling molecules that mediate short- and long-distance interactions between plants and microorganisms. Thus, VOCs-producing PGPB can be a natural and eco-friendly solution to increase agricultural production, since the bacteria do not need to colonize plant roots to trigger the growth promotion mechanisms. In our study, we aim to understand these mechanisms on Setaria viridis, a model plant of C4 metabolism (a close relative of sugarcane and corn), by investigating its molecular, cellular, physiological and phenotypical changes trigged by these bioactive compounds. Remarkably, we screened and selected two bacteria capable of increasing biomass of S. viridis plants in up to 4-fold in a co-cultivation system, wherein plant and bacteria only share the same atmosphere. The GC-MS analysis revealed that the volatilome of these bacteria include various compounds of several chemical classes, such as acids, alkenes, alcohols, ketones and thiols. Using a multi-omic approach (transcriptome, metabolome and proteome) to compare plants cultivated with the VOC-producer PGPB and the mock control, we obtained results that indicate possible pathways involved in growth promotion, such as cell wall modulation by the action of expansins and glycoside hydrolases, conversion of phenylalanine to cinnamic acid for lignin biosynthesis, modulation of the energy metabolism (higher concentrations of sugars and differential expression of TCA enzymes), and hormonal signaling (differential expression of enzymes involved in auxin, gibberellin and abscisic acid pathways). Furthermore, the results also indicate a pathogen defense response triggered by VOCs due to the higher expression of genes involved in such process. The ongoing steps seek to validate some of these multi-omic results, using different techniques such as Western-Blot, chlorophyll quantification, and co-cultivation assays with the plant media supplemented with sucrose or phytohormones. Our results of this project will provide a deep understanding of plant and bacterial interactions via VOCs. In the future, all this knowledge can contribute to developing a more sustainable agriculture.

Título do Evento: V GBMeeting
Cidade do Evento: Campinas
Título dos Anais do Evento: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora: Even3
Meio de Divulgação: Meio Digital
DOI: Obter o DOI

Como citar

ALMEIDA, Octávio Augusto Costa et al.. MULTI-OMIC APPROACH INDICATES THAT BACTERIAL VOLATILE ORGANIC COMPOUNDS PROMOTE SETARIA VIRIDIS GROWTH MAINLY VIA ENERGY METABOLISM, HORMONAL SIGNALING AND CELL WALL MODULATION.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609265-MULTI-OMIC-APPROACH-INDICATES-THAT-BACTERIAL-VOLATILE-ORGANIC-COMPOUNDS-PROMOTE-SETARIA-VIRIDIS-GROWTH-MAINLY-VIA. Acesso em: 27/07/2024