MULTI-OMIC APPROACH INDICATES THAT BACTERIAL VOLATILE ORGANIC COMPOUNDS PROMOTE SETARIA VIRIDIS GROWTH MAINLY VIA ENERGY METABOLISM, HORMONAL SIGNALING AND CELL WALL MODULATION

Publicado em 09/06/2023 - ISBN: 978-85-5722-785-9

Título do Trabalho
MULTI-OMIC APPROACH INDICATES THAT BACTERIAL VOLATILE ORGANIC COMPOUNDS PROMOTE SETARIA VIRIDIS GROWTH MAINLY VIA ENERGY METABOLISM, HORMONAL SIGNALING AND CELL WALL MODULATION
Autores
  • Octávio Augusto Costa Almeida
  • Bruno Henrique Silva Dias
  • Natália Oliveira de Araujo
  • Gabriela Persinoti
  • Lucas Ferreira Maciel
  • Celio Cabral Oliveira
  • Juliana Aparecida Aricetii
  • Maurício Luís Sforça
  • Maria Juliana Calderan Rodrigues
  • Juliana Velasco de Castro Oliveira
Modalidade
Apresentação de Pôster
Área temática
Genética de Microrganismos
Data de Publicação
09/06/2023
País da Publicação
Brasil
Idioma da Publicação
Inglês
Página do Trabalho
https://www.even3.com.br/anais/v-gbmeeting/609265-multi-omic-approach-indicates-that-bacterial-volatile-organic-compounds-promote-setaria-viridis-growth-mainly-via
ISBN
978-85-5722-785-9
Palavras-Chave
volatile organic compounds, plant growth promotion, multi-omics, sustainability
Resumo
Some plant growth promoting bacteria (PGPB) emit volatile organic compounds (VOCs), which are small gaseous signaling molecules that mediate short- and long-distance interactions between plants and microorganisms. Thus, VOCs-producing PGPB can be a natural and eco-friendly solution to increase agricultural production, since the bacteria do not need to colonize plant roots to trigger the growth promotion mechanisms. In our study, we aim to understand these mechanisms on Setaria viridis, a model plant of C4 metabolism (a close relative of sugarcane and corn), by investigating its molecular, cellular, physiological and phenotypical changes trigged by these bioactive compounds. Remarkably, we screened and selected two bacteria capable of increasing biomass of S. viridis plants in up to 4-fold in a co-cultivation system, wherein plant and bacteria only share the same atmosphere. The GC-MS analysis revealed that the volatilome of these bacteria include various compounds of several chemical classes, such as acids, alkenes, alcohols, ketones and thiols. Using a multi-omic approach (transcriptome, metabolome and proteome) to compare plants cultivated with the VOC-producer PGPB and the mock control, we obtained results that indicate possible pathways involved in growth promotion, such as cell wall modulation by the action of expansins and glycoside hydrolases, conversion of phenylalanine to cinnamic acid for lignin biosynthesis, modulation of the energy metabolism (higher concentrations of sugars and differential expression of TCA enzymes), and hormonal signaling (differential expression of enzymes involved in auxin, gibberellin and abscisic acid pathways). Furthermore, the results also indicate a pathogen defense response triggered by VOCs due to the higher expression of genes involved in such process. The ongoing steps seek to validate some of these multi-omic results, using different techniques such as Western-Blot, chlorophyll quantification, and co-cultivation assays with the plant media supplemented with sucrose or phytohormones. Our results of this project will provide a deep understanding of plant and bacterial interactions via VOCs. In the future, all this knowledge can contribute to developing a more sustainable agriculture.
Título do Evento
V GBMeeting
Cidade do Evento
Campinas
Título dos Anais do Evento
Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora
Even3
Meio de Divulgação
Meio Digital
DOI
LinkObter o DOI

Como citar

ALMEIDA, Octávio Augusto Costa et al.. MULTI-OMIC APPROACH INDICATES THAT BACTERIAL VOLATILE ORGANIC COMPOUNDS PROMOTE SETARIA VIRIDIS GROWTH MAINLY VIA ENERGY METABOLISM, HORMONAL SIGNALING AND CELL WALL MODULATION.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609265-MULTI-OMIC-APPROACH-INDICATES-THAT-BACTERIAL-VOLATILE-ORGANIC-COMPOUNDS-PROMOTE-SETARIA-VIRIDIS-GROWTH-MAINLY-VIA. Acesso em: 23/06/2024

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