ARABIDOPSIS THALIANA BZIP TRANSCRIPTION FACTORS ARE INVOLVED IN THE MANAGEMENT OF ENERGY RESOURCES

Raphael de Araújo Campos; AMÉRICO JOSÉ CARVALHO VIANA; João Guilherme Portugal Vieira; Pamela Tavares Carlson; Thyelen Engel de Jesus; Michel Georges Albert Vincentz

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Trabalho

Título do Trabalho

ARABIDOPSIS THALIANA BZIP TRANSCRIPTION FACTORS ARE INVOLVED IN THE MANAGEMENT OF ENERGY RESOURCES

Autores

Raphael de Araújo Campos
AMÉRICO JOSÉ CARVALHO VIANA
João Guilherme Portugal Vieira
Pamela Tavares Carlson
Thyelen Engel de Jesus
Michel Georges Albert Vincentz

Modalidade

Apresentação Oral

Área temática

Genética Vegetal e Melhoramento

Data de Publicação

09/06/2023

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/v-gbmeeting/609261-arabidopsis-thaliana-bzip-transcription-factors-are-involved-in-the-management-of-energy-resources

ISBN

978-85-5722-785-9

Palavras-Chave

Arabidopsis thaliana, bZIP transcription factors, Starch

Resumo

Carbohydrates, resulting from CO2 fixation through photosynthesis, represent the main source of energy used by plants to carry out their vital functions during the day. At night, starch, reserve carbohydrate synthethised during the day, is degraded at a rate compatible with a regular supply of carbohydrates to sustain growth until dawn. The Arabidopsis thaliana group C basic leucine zipper (bZIP) transcription factor (TF) bZIP63 is involved in the regulation of starch breakdown. bZIP63 was shown to preferentially heterodimerize with group S1 bZIPs, including bZIP1 and bZIP53 TFs. Heterodimerization results in transcriptional rewiring, leading to changes on target genes expression and hence on energy balance. Although the knowledge on how plants optimize the usage of their energy resources to grow and develop is of great interest for a yield crops improvement, little is known about this. Thus, the objective of the Ph.D. intends to investigate the role of TFs bZIP1, 53 and 63 in controlling the usage of energy resources. For this, analysis of changes in growth, developmental, metabolism and gene expression during the diel cycle in single, double and triple mutants for bZIP1, 53 and 63 are being developed. Additionally, we are analyzing two silenced lines (L9 and L5) for expression of bZIP63 (~10% compared to WT), bZIP1 (38% compared to WT) and bZIP53 (60 % compared to WT) by interference RNA (RNAi). Gene expression analysis by RNAseq and RT-qPCR between wild type (WT) and mutants RNAiL9 and L5 showed that genes related to starch, sugar and amino acids metabolism and short day growth control were specifically deregulated in these silenced lines. Interestingly, BETA-AMYLASE4 (BAM4) gene, which encodes a protein related to starch breakdown regulation, is strongly repressed (24-fold change) in RNAi L9 and L5 lines versus WT. In addition, RNAiL9 and L5 plants end the night with a starch excess phenotype, probably due the repression of BAM4. Aside of that, RNAiL9 mutants lines grew about 25% (p<0.05) less than WT, suggesting an inefficiency in the management of energy resources which impairs growth. These data further support the role of bZIP1/53 and 63 partnership in defining the gene expression program related to energy homeostasis. Henceforth, we intend to investigate growth, transcript levels of target genes and the dynamics of proteins throughout the diel cycle in single, double and triple mutants for bZIP1, 53 and 63.

Título do Evento: V GBMeeting
Cidade do Evento: Campinas
Título dos Anais do Evento: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora: Even3
Meio de Divulgação: Meio Digital
DOI: Obter o DOI

Como citar

CAMPOS, Raphael de Araújo et al.. ARABIDOPSIS THALIANA BZIP TRANSCRIPTION FACTORS ARE INVOLVED IN THE MANAGEMENT OF ENERGY RESOURCES.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609261-ARABIDOPSIS-THALIANA-BZIP-TRANSCRIPTION-FACTORS-ARE-INVOLVED-IN-THE-MANAGEMENT-OF-ENERGY-RESOURCES. Acesso em: 27/07/2024