HIGH-DENSITY AND MULTILOCUS LINKAGE MAP FOR UROCHLOA HUMIDICOLA REVEALS INSIGHTS INTO THE ORIGIN AND APOMIXIS CONTROL

Aline Da Costa Lima Moraes; Bianca Baccili Zanotto Vigna; Anete Pereira de Souza

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Trabalho

Título do Trabalho

HIGH-DENSITY AND MULTILOCUS LINKAGE MAP FOR UROCHLOA HUMIDICOLA REVEALS INSIGHTS INTO THE ORIGIN AND APOMIXIS CONTROL

Autores

Aline Da Costa Lima Moraes
Bianca Baccili Zanotto Vigna
Anete Pereira de Souza

Modalidade

Apresentação de Pôster

Área temática

Genética Vegetal e Melhoramento

Data de Publicação

09/06/2023

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/v-gbmeeting/609210-high-density-and-multilocus-linkage-map-for-urochloa-humidicola-reveals-insights-into-the-origin-and-apomixis-con

ISBN

978-85-5722-785-9

Palavras-Chave

forage grasses, SNP, GBS, linkage mapping, polyploid

Resumo

Pastures based on forage grasses have a great impact on the world’s economy. The livestock sector and associated market chains employ 1.3 billion people worldwide and contribute with the production of two important agricultural commodities with high global economic value (milk and beef). Tropical forage grasses are mainly grown in Africa and South America, and the african species Urochloa humidicola (Rendle) Morrone & Zuloaga (syn. Brachiaria humidicola (Rendle) Schweick.) have been planted in wet regions and low-fertility acidic soils, as the wetlands of the Pantanal and the Amazon Basin. Despite such importance, few cultivars of U. humidicola are commercially available (Tully, Llanero and BRS Tupi). As part of the breeding program developed by Embrapa Gado de Corte, the sexual accession H031 was crossed with the commercial apomictic cultivar BRS Tupi, both hexaploid, generating 279 F1 hybrids (2n=6x=36). Using this intraspecific progeny, the aim of the present study is to increase the knowledge of the genetics and genomic structure of the tropical forage U. humidicola, by building a high-resolution and integrated linkage map with allele dosage information for the species. From the parents and the full-sib progeny, we constructed GBS libraries and performed SNP calling using the Urochloa ruziziensis genome as reference. In this step, we identified 62 contaminating individuals in the progeny, which were excluded and SNP calling was performed again. The ploidy and dosage of each bi-allelic loci was estimated by the SuperMASSA software and, after filtrations, 7069 SNP markers were used to construct the linkage map using the MapPoly R package. SNPs were grouped and ordered using a de novo map construction approach, and improvements were performed with the U. ruziziensis reference genome to generate the final map with 4883 markers distributed in 36 linkage groups (LGs) and 854.63 cM. From the map, it was possible to infer the haplotypes of parents and hybrids, and evaluate the occurrence of preferential pairing in the parents of the progeny. We observed a significant level of preferential pairing between the two homologous in cv. Tupi and no significant preferential pairing across the whole genome of sexual genotype H031, which corroborates with previous hypotheses described in literature that this access has a different origin. We also evaluated all individuals with p778/779, a primer pair within the Pennisetum squamulatum (L.) R.Br. ASGR-BABY BOOM-like (PsASGR-BBML) candidate gene for one component of apomixis, and this marker was mapped together with the phenotype in LG3. The obtained results provided an important advance in the knowledge of the genetics and genomic organization of the tropical forage U. humidicola and the sexual accession H031. Consequently, all this information may help in new selection strategies to increase the efficiency of breeding programs for the species.

Título do Evento: V GBMeeting
Cidade do Evento: Campinas
Título dos Anais do Evento: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora: Even3
Meio de Divulgação: Meio Digital
DOI: Obter o DOI

Como citar

MORAES, Aline Da Costa Lima; VIGNA, Bianca Baccili Zanotto; SOUZA, Anete Pereira de. HIGH-DENSITY AND MULTILOCUS LINKAGE MAP FOR UROCHLOA HUMIDICOLA REVEALS INSIGHTS INTO THE ORIGIN AND APOMIXIS CONTROL.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609210-HIGH-DENSITY-AND-MULTILOCUS-LINKAGE-MAP-FOR-UROCHLOA-HUMIDICOLA-REVEALS-INSIGHTS-INTO-THE-ORIGIN-AND-APOMIXIS-CON. Acesso em: 27/07/2024