MOLECULAR PHENOTYPES AT THE GENE ISOFORM LEVEL ASSOCIATED WITH MULTIPLE SALT STRESSES IN THE EXTREMOPHYTE MODEL SCHRENKIELLA PARVULA

Michele Fernandes da Silva; Chathura Wijesinghege; Maheshi Dassanayake; Anete Pereira de Souza

Título do Trabalho

Autores

Michele Fernandes da Silva
Chathura Wijesinghege
Maheshi Dassanayake
Anete Pereira de Souza

Modalidade

Apresentação de Pôster

Área temática

Genética Vegetal e Melhoramento

Data de Publicação

09/06/2023

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/v-gbmeeting/608755-molecular-phenotypes-at-the-gene-isoform-level-associated-with-multiple-salt-stresses-in-the-extremophyte-model-s

ISBN

978-85-5722-785-9

Palavras-Chave

co-expression networks, WGCNA, halophyte, tissue-specific

Resumo

How the diversity of alternatively spliced isoforms contribute to adaptation to extreme environments is a fundamental topic in evolutionary biology. Located on the shores of Lake Tuz, Turkey, where soils accumulate high concentrations of multi-ion salts, Schrenkiella parvula is a robust salt-tolerant extremophyte model for investigating multiple abiotic stresses. In recent years, fundamental genomic resources have been created for S. parvula. However, transcript diversity enabled by alternatively spliced isoforms and their spatiotemporal presence and interactions are poorly explored. We investigated the potential molecular mechanisms and expression patterns at the isoform level associated with multiple salt stresses in S. parvula. Using full-length reference transcripts generated by Iso-Seq and a spatiotemporal expression profile generated from 107 RNA-Seq samples including different tissues and developmental stages, we quantified the expression of > 70,000 isoforms and identified those that respond to tissue-specific saline stress. Differentially expressed isoforms were enriched in functional categories related to metabolic, biosynthetic and homeostasis processes, transport, protein folding and stress response. We built a global co-expression network to deduce the main metabolic pathways and isoform-specific tissue responses that may govern salinity tolerance throughout the lifecycle of this extremophyte. Insight gained from this work will advance our knowledge about the adaptive evolution of extremophytes, especially in the face of climate change.

Título do Evento: V GBMeeting
Cidade do Evento: Campinas
Título dos Anais do Evento: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora: Even3
Meio de Divulgação: Meio Digital
DOI: Obter o DOI

Como citar

SILVA, Michele Fernandes da et al.. MOLECULAR PHENOTYPES AT THE GENE ISOFORM LEVEL ASSOCIATED WITH MULTIPLE SALT STRESSES IN THE EXTREMOPHYTE MODEL SCHRENKIELLA PARVULA.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/608755-MOLECULAR-PHENOTYPES-AT-THE-GENE-ISOFORM-LEVEL-ASSOCIATED-WITH-MULTIPLE-SALT-STRESSES-IN-THE-EXTREMOPHYTE-MODEL-S. Acesso em: 27/07/2024