THE RNA BINDING PROTEIN BICC1 ASSOCIATES WITH THE RIBOSOME AND INHIBITS PROTEINS TRANSLATION

Publicado em 09/06/2023 - ISBN: 978-85-5722-785-9

Título do Trabalho
THE RNA BINDING PROTEIN BICC1 ASSOCIATES WITH THE RIBOSOME AND INHIBITS PROTEINS TRANSLATION
Autores
  • Heloísa Monteiro do Amaral Prado
  • Gabriela Alves Moreira
  • Mario H. Bengtson
  • Katlin Brauer Massirer
Modalidade
Apresentação de Pôster
Área temática
Genética Animal e Evolução
Data de Publicação
09/06/2023
País da Publicação
Brasil
Idioma da Publicação
Inglês
Página do Trabalho
https://www.even3.com.br/anais/v-gbmeeting/608521-the-rna-binding-protein-bicc1-associates-with-the-ribosome-and-inhibits-proteins-translation
ISBN
978-85-5722-785-9
Palavras-Chave
RNA-binding protein, co-immunoprecipitation, ribosome, polysome profiling, translation.
Resumo
Introduction: The Protein Bicaudal C Homolog 1 (BICC1) is a very conserved RNA-binding protein (RBP). Structurally, the BICC1 protein is notorious for presenting three K Homology domains at the N-terminal end (KH1, KH2, and KH3 domains), and a Sterile Alpha Motif (SAM domain) at the C-terminal end. The studies about BICC1 started when a disruption in a locus of Drosophila lead to an abnormal anterior-posterior phenotype (abdomen “bicaudal”). In mammals, mutations in BICC1 are related to polycystic kidney disease (PKD) development and renal organogenesis, and to the hyperactivity of the cytogenetics Wnt ß-catenin pathway. Recently, studies have also pointed to differential levels of BICC1 in types of cancer and mood disorders. However, there is still a lack of studies about BICC1 and molecular mechanisms regulated by its expression, as well as by its association with RNA targets and protein partners. Therefore, the comprehensive characterization of the protein interactome of this RBP is very informative for understanding the protein function and advancing its knowledge in disease contexts. Methodology: We have characterized protein interactors (interactome) through ectopic overexpression of BICC1 in HEK293T cells followed by co-immunoprecipitation and mass spectrometry (Co-IP-MS). Based on the interactome results, we are currently investigating the role of BICC1 in translation activity by polysome profiling and puromycin assays. Results: Within the 180 proteins that were co-immunoprecipitated with BICC1-Flag, we observed enrichment in proteins related to ribosome biogenesis and translation activity. Our results from polysome profiling suggest a negative role of BICC1 in cellular translation, and the puromycin assays indicate a possible role of the SAM domain in protein synthesis in cells. Conclusion and perspectives: From Co-IP-MS results, we have selected a cellular pathway to pursue and associate with BICC1 expression, which is the cellular translation pathway. Our results point to the BICC1 role in the translation activity, possibly a negative role. As the next steps, as BICC1 is RNA binding protein and our group has data about new RNA targets and protein partners of BICC1, we aim to connect this data with the regulation of the well-characterized Wnt ß-catenin pathway, aiming this RBP as a potential drug target for human diseases, such as cancer and PKD. Fontes de apoio financeiro: Fapesp (21/04867-5).
Título do Evento
V GBMeeting
Cidade do Evento
Campinas
Título dos Anais do Evento
Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora
Even3
Meio de Divulgação
Meio Digital

Como citar

PRADO, Heloísa Monteiro do Amaral et al.. THE RNA BINDING PROTEIN BICC1 ASSOCIATES WITH THE RIBOSOME AND INHIBITS PROTEINS TRANSLATION.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/608521-THE-RNA-BINDING-PROTEIN-BICC1-ASSOCIATES-WITH-THE-RIBOSOME-AND-INHIBITS-PROTEINS-TRANSLATION. Acesso em: 04/11/2024

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