CHROMOSOMAL EVOLUTION AND EVOLUTIONARY RELATIONSHIPS OF THE GENUS LEBIASINA (TELEOSTEI, CHARACIFORMES)

Francisco de Menezes Cavalcante Sassi; Ezequiel Aguiar de Oliveira; Luiz Antonio Carlos Bertollo; Terumi Hatanaka; Mauro Nirchio Tursellino; Manoela M. F. Marinho; MARCELO DE BELLO CIOFFI

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Trabalho

Título do Trabalho

CHROMOSOMAL EVOLUTION AND EVOLUTIONARY RELATIONSHIPS OF THE GENUS LEBIASINA (TELEOSTEI, CHARACIFORMES)

Autores

Francisco de Menezes Cavalcante Sassi
Ezequiel Aguiar de Oliveira
Luiz Antonio Carlos Bertollo
Terumi Hatanaka
Mauro Nirchio Tursellino
Manoela M. F. Marinho
MARCELO DE BELLO CIOFFI

Modalidade

Resumo

Área temática

Citogenética Animal

Data de Publicação

13/02/2020

País da Publicação

Brasil

Idioma da Publicação

pt-BR

Página do Trabalho

https://www.even3.com.br/anais/rbcc2019/160097-chromosomal-evolution-and-evolutionary-relationships-of-the-genus-lebiasina-(teleostei-characiformes)

ISBN

978-85-5722-425-4

Palavras-Chave

Cytogenetics, CGH, Zoo-FISH

Resumo

The Lebiasinidae family is composed by seven genus of minute fishes living in small rivers of Central and South America, with a great diversity in shape and body color. However, their evolutionary relationships are still unclear. Here we present, for the first time, cytogenetic data of two Lebiasina species (L. bimaculata and L. melanoguttata) using multipronged cytogenetic approaches including C- and CMA3 banding, repetitive DNAs mapping, CGH and Zoo-FISH experiments. We aimed i) to investigate evolutionary events inside Lebiasina genus and their relation with other Lebiasinidae genera and ii) to check whether cytogenetic data supports evolutionary relationships between Lebiasinidae and Ctenoluciidae, as proposed for recent molecular phylogeny studies. Both Lebiasina species have the same diploid number (2n=36), with karyotypes composed exclusively by bi-armed chromosomes and similar microsatellite distribution pattern. However, both species present contrasting patterns of C-positive heterochromatin and CMA3+ bands. The remarkable interstitial series of C-positive heterochromatin occurring in L. melanoguttata is absent in L. bimaculata. Accordingly, L. bimaculata shows the rDNA sites as the only GC-rich (CMA3+) regions in the chromosomes. On other hand, L. melanoguttata evidences a clear CMA3+ intercalated banding pattern. In addition, the chromosomal mapping of rDNAs showed a different pattern between both species, with multiple 5S and 18S rDNA sites in L. melanogutatta in contrast with single ones in L. bimaculata. CGH experiments also revealed a high level of genomic differentiation between both Lebiasina species. Notably, a polymorphic state of a conspicuous C-positive, CMA3+ and (CGG)n band was found only to occur in L. bimaculata females. However, whether such genomic divergence, also highlighted by the CGH experiments, represents a nascent sex chromosome system or is related to a copy number variation processis not yet clear. Besides possessing the same diploid number, the WCP and CGH experiments indicate that Lebiasina and Boulengerella maculata share similar chromosomal sequences, thus supporting relatedness among them and evolutionary relationships between the Lebiasinidae and Ctenoluciidae families.

Título do Evento: VI Reunião Brasileira de Citogenética e Citogenômica
Cidade do Evento: Goiânia
Título dos Anais do Evento: Anais da VI Reunião Brasileira de Citogenética e Citogenômica
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

SASSI, Francisco de Menezes Cavalcante et al.. CHROMOSOMAL EVOLUTION AND EVOLUTIONARY RELATIONSHIPS OF THE GENUS LEBIASINA (TELEOSTEI, CHARACIFORMES).. In: Anais da VI Reunião Brasileira de Citogenética e Citogenômica. Anais...Goiânia(GO) Hotel Mercure, 2019. Disponível em: https//www.even3.com.br/anais/rbcc2019/160097-CHROMOSOMAL-EVOLUTION-AND-EVOLUTIONARY-RELATIONSHIPS-OF-THE-GENUS-LEBIASINA-(TELEOSTEI-CHARACIFORMES). Acesso em: 04/04/2026