Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)

PRELIMINARY ANALYSIS OF CHEMICAL ELEMENTS INVOLVED IN THE INFECTION OF AEDES AEGYPTI LARVAE BY METARHIZIUM ANISOPLIAE

Publicado em 10/03/2025 - ISBN: 978-65-272-1246-1

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Título do Trabalho
PRELIMINARY ANALYSIS OF CHEMICAL ELEMENTS INVOLVED IN THE INFECTION OF AEDES AEGYPTI LARVAE BY METARHIZIUM ANISOPLIAE
Autores
  • Kamila Leite de Amorim Magalhães
  • Adriani da Silva Carneiro Lopes
  • Sara Ferreira Abud
  • Haika Victória Sales Moreira
  • Victor Hugo Machado Luques Dias
  • Patricia Silva Golo
  • Vânia Rita Elias Pinheiro Bittencourt
  • Marcos Vinicius Colaço Gonçalves
  • Huarrisson Azevedo Santos
  • Isabele da Costa Angelo
Modalidade
Resumo
Área temática
Microbiologia e imunologia
Data de Publicação
10/03/2025
País da Publicação
Brasil
Idioma da Publicação
Inglês
Página do Trabalho
https://www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1032145-preliminary-analysis-of-chemical-elements-involved-in-the-infection-of-aedes-aegypti-larvae-by-metarhizium-aniso
ISBN
978-65-272-1246-1
Palavras-Chave
SEM, X-ray Spectrometry, Chemical Elements, Immune Response
Resumo
This study aimed to identify and analyze the chemical elements involved in the infectious process of Aedes aegypti larvae exposed to the entomopathogenic fungus Metarhizium anisopliae using Scanning Electron Microscopy (SEM) with Energy-Dispersive X-ray Spectroscopy (EDS). Ae. aegypti larvae were treated with 0.03% Tween 80 (control group), with M. anisopliae isolate CG153 (105 and 107 conidia/mL), and a group remained untreated (naive). 24, 48, and 72 hours post-infection, the larvae were fixed in glutaraldehyde solution (2.5%, pH 7.2) and stored at -4º C. The process continued with three washes in 0.1M sodium cacodylate buffer (CS) for 10 minutes each, fixation in osmium tetroxide for one hour, followed by three additional washes in 0.1M CS. Next, the samples were dehydrated in an ascending alcohol series (7.5%, 15%, 30%, 50%, 70%, 80%, 90%, and 100%) for ten minutes each. After this process, the samples were dried in CO2 and coated with carbon. For image visualization and SEM-EDS analysis, the JEOL JSM-6390LV scanning electron microscope (JEOL BRAZIL©, São Paulo, Brazil), from the FIOCRUZ microscopy platform, and the Phenom ProX microscope (Thermo Fisher Scientific©) from the Multiuser Ultrastructure Laboratory (LMU) at the Federal Rural University of Rio de Janeiro (UFRRJ) were used. A preliminary analysis demonstrated that the naive control group exhibited a composition rich in carbon (C) and oxygen (O), essential elements for the natural development of the larvae, as well as elements such as phosphorus (P), nitrogen (N), and sulfur (S), necessary for metabolism and cellular structure. The presence of aluminum (Al) indicated possible contamination from the stubs supporting the samples. In the groups treated with 0.03% Tween 80, the chemical composition remained similar to the naive group, with no significant changes in the basic elements (C, O, N, P, and S). This suggests that 0.03% Tween 80 did not cause relevant alterations in the larvae's structure or metabolism, maintaining a chemical profile close to the naive control. On the other hand, the groups infected with M. anisopliae showed remarkable changes. After 24 hours at a concentration of 107 conidia/mL, there was a marked increase in C (69.6%) and a reduction in N (4.1%), suggesting that the fungus altered the organic composition of the larvae and interfered with protein metabolism. Furthermore, oxygen content decreased to 23.3% by weight, possibly due to the high oxygen consumption by metabolic processes involved in the immune response. In the groups treated for 48 and 72 hours, the elements P and S showed slight increases, reflecting an immune response by the larvae, mobilizing energy reserves and structural proteins to resist the infection. The 0.03% Tween 80 emulsifier did not significantly affect the larvae’s chemical composition, while the entomopathogenic fungus M. anisopliae substantially altered chemical elements involved in the metabolism and organic structure of Ae. aegypti larvae. A more detailed SEM-EDS analysis will be conducted to better understand the interaction between Ae. aegypti larvae and M. anisopliae, contributing to the development of mosquito control strategies.
Título do Evento
IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Título dos Anais do Evento
Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Nome da Editora
Even3
Meio de Divulgação
Meio Digital

Como citar

MAGALHÃES, Kamila Leite de Amorim et al.. PRELIMINARY ANALYSIS OF CHEMICAL ELEMENTS INVOLVED IN THE INFECTION OF AEDES AEGYPTI LARVAE BY METARHIZIUM ANISOPLIAE.. In: Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS). Anais...Seropédica(RJ) UFRRJ, 2025. Disponível em: https//www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1032145-PRELIMINARY-ANALYSIS-OF-CHEMICAL-ELEMENTS-INVOLVED-IN-THE-INFECTION-OF-AEDES-AEGYPTI-LARVAE-BY-METARHIZIUM-ANISO. Acesso em: 10/12/2025

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