Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)

CHARACTERIZATION OF FELINE MACROPHAGES OF THE FCWF-4 CELL LINE INFECTED IN THE L. AMAZONENSIS PARASITE.

Publicado em 10/03/2025 - ISBN: 978-65-272-1246-1

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Trabalho
Título do Trabalho
CHARACTERIZATION OF FELINE MACROPHAGES OF THE FCWF-4 CELL LINE INFECTED IN THE L. AMAZONENSIS PARASITE.
Autores
  • Fernanda de Paula Pepino Moreira
  • Nicolle De Lacerda Belarmino
  • Luciana Pinheiro
  • Renata Quintanilha dos Santos
  • Letícia Seabra Abrantes
  • Lucia Mendonça Previato
  • Jose Osvaldo Previato
  • Celio Geraldo Freire De Lima
  • Debora Decote Ricardo de Lima
  • Lúcia Helena Pinto da Silva
Modalidade
Resumo
Área temática
Protozoários e Agentes transmitidos por vetores de importância em Saúde
Data de Publicação
10/03/2025
País da Publicação
Brasil
Idioma da Publicação
Inglês
Página do Trabalho
https://www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1028290-characterization-of-feline-macrophages-of-the-fcwf-4-cell-line-infected-in-the-l-amazonensis-parasite
ISBN
978-65-272-1246-1
Palavras-Chave
feline macrophage; leishmaniasis; immunomodulation
Resumo
Leishmaniasis is a disease caused by parasites of the Leishmania genus, transmitted to humans and animals through the bites of infected sandflies. While it predominantly affects humans and dogs, cases of cat leishmaniasis have been reported, particularly in regions where the disease is endemic, and it is usually associated with other pathogens co-infection. Some works suggest that an immunodepression could become cats more susceptible to Leishmania infection. The main of our work was to evaluate the interaction of a feline macrophage cell line Fcwf-4 and Leishmania parasite in vitro, since the regulatory mechanisms of macrophages response can vary depending on species. Besides that, we analyzed if the presence of immunomodulatory molecules such as fungal polysaccharide glucuronoxilomannan (GXM) from Cryptococcus neoformans could immunomodulate feline macrophage function during Leishmania infection. First, we analyze GXM toxicity for Fcwf-4 feline macrophages, the preliminary results showed until 100ng/mL of GXM treatment, feline macrophages remained viable. Then, Fcwf-4 feline macrophages pre-treated or not with GXM were infected with Leishmania amazonensis promastigotes, the data showed that after 2 hours of infection 60% of feline macrophages were infected, interestingly, the presence of GXM capsule reduce to 40% the phagocytic ability of feline macrophages. However, the percentage of infected macrophages after 48 hours of infection augmented 2.45-fold in GXM-treated feline macrophages in comparison to untreated ones. Next, we are going to evaluate reactive oxygen and nitrogen species produced by untreated and treated-GXM feline macrophage Leishmania infected or not. Feline leishmaniasis presents a significant concern for cat owners, especially in regions where the disease is prevalent. Understanding the mechanism of feline macrophage biology and its differences is essential for researchers to study immune responses, and disease mechanisms.
Título do Evento
IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Título dos Anais do Evento
Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Nome da Editora
Even3
Meio de Divulgação
Meio Digital

Como citar

MOREIRA, Fernanda de Paula Pepino et al.. CHARACTERIZATION OF FELINE MACROPHAGES OF THE FCWF-4 CELL LINE INFECTED IN THE L. AMAZONENSIS PARASITE... In: Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS). Anais...Seropédica(RJ) UFRRJ, 2025. Disponível em: https//www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1028290-CHARACTERIZATION-OF-FELINE-MACROPHAGES-OF-THE-FCWF-4-CELL-LINE-INFECTED-IN-THE-L-AMAZONENSIS-PARASITE. Acesso em: 07/12/2025

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