Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)

MORPHOLOGICAL, MORPHOMETRIC, AND MOLECULAR ANALYSES OF HEPATOZOON SP. FROM WILD RODENTS IN CRUZ MACHADO, PARANÁ

Publicado em 10/03/2025 - ISBN: 978-65-272-1246-1

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Título do Trabalho
MORPHOLOGICAL, MORPHOMETRIC, AND MOLECULAR ANALYSES OF HEPATOZOON SP. FROM WILD RODENTS IN CRUZ MACHADO, PARANÁ
Autores
  • Tatiana Pádua Tavares De Freitas
  • Maristela Peckle Peixoto
  • Eduarda de Oliveira Silva Lima Machado
  • Bernardo Rodrigues Teixeira
  • Isaac Leandro Lira Pinto
  • Fernando de Oliveira Santos
  • Silmara Aparecida Ferreira de Carvalho
  • Laís Da Silva De Oliveira
  • Huarrisson Azevedo Santos
  • Carlos Luiz Massard
Modalidade
Resumo
Área temática
Protozoários e Agentes transmitidos por vetores de importância em Saúde
Data de Publicação
10/03/2025
País da Publicação
Brasil
Idioma da Publicação
Inglês
Página do Trabalho
https://www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1028107-morphological-morphometric-and-molecular-analyses-of-hepatozoon-sp-from-wild-rodents-in-cruz-machado-parana
ISBN
978-65-272-1246-1
Palavras-Chave
Rodents, Hepatozoon, Phylogenetic analysis, gametocytes
Resumo
Protozoa of the genus Hepatozoon infect all vertebrate orders, serving as intermediate hosts for various vertebrates and definitive hosts for hematophagous invertebrates. Infections in rodents have been reported worldwide, suggesting their role as paratenic hosts due to the presence of parasite cysts in their tissues. This study aimed to detect Hepatozoon spp. in liver samples and blood smears from 52 rodents (26 Akodon montensis, 12 Oligoryzomys nigripes, one Mus musculus, 11 Sooretamys angouya, and two Rattus rattus) captured in Cruz Machado, Paraná, in December 2021. Capture was authorized by ICMBio (license number 13373-2), and procedures were approved by the Fiocruz CEUA license (L-036/2018). Genomic DNA was isolated using the DNeasy Blood and Tissue kit (Qiagen), standardized to a concentration of 60 ng/mL, and subjected to polymerase chain reactions (PCR) with primers Hep300F and Hep900R targeting the 18S rDNA. Amplified products (~600 bp) were purified (Illustra GFX GE kit) and sequenced using the Sanger method. The obtained sequences were edited using Geneious Primer 2023.0 and aligned in Mega11 with other Hepatozoon spp., Haemogregarina spp., Karyolysus paradoxa, and Dactylosoma ranarum (outgroup) sequences from GenBank. Phylogenetic trees based on 522 bp were generated through Bayesian inference using two independent runs of 10 million generations of MCMC with a 25% burn-in in MrBayes. The consensus tree was visualized in FigTree 1.4.1 and edited in Inkscape 1.3. Blood smears were analyzed using an Olympus BX-51 microscope, and gametocyte measurements were obtained using CellSens® software. PCR results indicated 30 positive animals: 18 A. montensis, six O. nigripes, one M. musculus, and five S. angouya, yielding an overall prevalence of Hepatozoon spp. infection of 57.7% (30/52). Among the sampled species, prevalence was 69.2% (18/26) for A. montensis, 100% (1/1) for M. musculus, 50% (6/12) for O. nigripes, and 45.4% (5/11) for S. angouya, with no detection in R. rattus. Gametocytes were observed within monocytes or free in blood smears from 17 of 26 (65.38%) A. montensis and one of 12 (8.3%) O. nigripes. These gametocytes were elongated to oval, with light blue coloration and slightly condensed nuclei, positioned centrally or at one end. Length and width measurements of the capsules and nuclei of ten gametocytes per animal were recorded. The average lengths and widths of the gametocytes in A. montensis were 10.95 µm and 4.52 µm, respectively, with nuclei measuring 5.89 µm and 4.44 µm. These morphometric results were consistent with those previously described for Hepatozoon milleri. Phylogenetic analysis revealed a separation between Hepatozoon spp. detected in carnivores and those found in rodents, marsupials, amphibians, and reptiles. The sequences from this study clustered in a subclade with the representative sequence of H. milleri found in A. montensis (KU736608) and sequences from Akodon sp. (OM033660 and OM033663) and Euryoryzomys russatus (OM033661 and OM033662). Based on these results, it is suggested that the analyzed parasites are H. milleri, expanding the known rodent species associated with this parasite. However, further analyses involving other life stages of the parasite are warranted. Studies combining morphological, morphometric, and phylogenetic analyses with other molecular targets are necessary to enhance understanding of this parasite and its potential implications for animal health and impacts on Brazilian biodiversity.
Título do Evento
IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Título dos Anais do Evento
Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Nome da Editora
Even3
Meio de Divulgação
Meio Digital

Como citar

FREITAS, Tatiana Pádua Tavares De et al.. MORPHOLOGICAL, MORPHOMETRIC, AND MOLECULAR ANALYSES OF HEPATOZOON SP. FROM WILD RODENTS IN CRUZ MACHADO, PARANÁ.. In: Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS). Anais...Seropédica(RJ) UFRRJ, 2025. Disponível em: https//www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1028107-MORPHOLOGICAL-MORPHOMETRIC-AND-MOLECULAR-ANALYSES-OF-HEPATOZOON-SP-FROM-WILD-RODENTS-IN-CRUZ-MACHADO-PARANA. Acesso em: 10/11/2025

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