IN VITRO ANALYSIS OF THE LEISHMANICIDAL ACTIVITY AND TOXICITY OF THE ESSENTIAL OIL OF SCHINUS TEREBINTHIFOLIUS RADDI

Bianca Augusto de Souza; Dayana Rosa; Pedro Corrêa Damasceno Junior; Patrícia Fampa Negreiros Lima; Douglas Siqueira de Almeida Chaves

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Título do Trabalho

IN VITRO ANALYSIS OF THE LEISHMANICIDAL ACTIVITY AND TOXICITY OF THE ESSENTIAL OIL OF SCHINUS TEREBINTHIFOLIUS RADDI

Autores

Bianca Augusto de Souza
Dayana Rosa
Pedro Corrêa Damasceno Junior
Patrícia Fampa Negreiros Lima
Douglas Siqueira de Almeida Chaves

Modalidade

Resumo

Área temática

Protozoários e Agentes transmitidos por vetores de importância em Saúde

Data de Publicação

10/03/2025

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1027206-in-vitro-analysis-of-the-leishmanicidal-activity-and-toxicity-of-the-essential-oil-of-schinus-terebinthifolius-r

ISBN

978-65-272-1246-1

Palavras-Chave

essential oils, visceral leishmaniasis, chemotherapy, brazilian peppertree.

Resumo

The leishmaniasis are a group of parasitic diseases, classified as neglected. They are caused by flagellated protozoa of the genus Leishmania and are transmitted by the bite of infected female phlebotomine vectors. In the epidemiological context, the Leishmania (Leishmania) infantum species causes the most severe form of the disease, called visceral. Natural products are alternative sources for the treatment of various affections and several studies have demonstrated the biological leishmanicidal activity of extracts and essential oils from a variety of plant species. The native Brazilian medicinal species, Schinus terebinthifolius Raddi, popularly known as brazilian peppertree, was selected for this study because it has several therapeutic applications and well-known properties, such as anti-inflammatory, healing and antimicrobial properties. The aim of the study was to biologically evaluate (leishmanicidal activity) the effect of essential oils (EOs) from 4 genotypes/chemotypes; ARO030-F; ARO071-F; ARO078-F; ARO136-F isolated from the species Schinus terebinthifolius (Anacardiaceae) against Leishmania infantum, as well as evaluating its toxicity against the cells of its vertebrate host in vitro. Four genotypes of S. terebinthifolius (ARO030-F, ARO071-F, ARO078-F and ARO136-F) deposited at germplasm bank of the Institute of Agronomy at UFRRJ were selected. Essential oils (EOs) from the chosen genotypes were obtained by the hydrodistillation method with a Clevenger apparatus, using 150g of dried and crushed leaves, as recommended by the International Organization for Standardization (ISO9235:2021). After 3h of hydrodistillation, the essential oils were stored in amber glass bottles under refrigeration (-20°C). The obtained oils were submitted to Gas chromatography coupled to a mass spectrometer and flame ionization detector (GC-MS and GC-DIC), and the main compounds for ARO030-F were identified: a-pinene (24.79%), limonene (17.26%) and d-2-carene (12.18%). For ARO071-F, a-pinene (36.74%), ?-cymene (36.22%) and caryophyllene oxide (5.54%). For ARO078-F, Sabinene (20.13%), Terpinen-4-ol (20.11%) and ?-terpinene (13.08%) and for ARO136-F, ß-pinene (16.53% ), a-terpineol (12.29%) and trans-caryophyllene (12.03%). For the in vitro leishmanicidal activity assay, promastigote forms in the logarithmic growth phase of L. infantum were cultivated in Schneider culture medium pH 7.2, supplemented with 10% Fetal Bovine Serum and penicillin-streptomycin 10,000 U-10mg/mL, maintained at 27°C. For the cytotoxicity (cell viability) assay, murine macrophages from the RAW 264.7 cell line were used, maintained in DMEM culture medium, supplemented with Glucose 4.5 g/L, 10% Fetal Bovine Serum and penicillin-streptomycin 10,000 U-10mg/mL, kept at 37°C and 5% CO2. To evaluate anti-promastigote activity and cell viability, the colorimetric method was carried out using the XTT reagent, which reveals mitochondrial dehydrogenase activity. All the EOs showed anti L. infantum promastigote activity with IC50 values of 32.46, 20.52, 41.61 and 32.62 µg/mL for ARO030-F, ARO071-F, ARO078-F and ARO136-F, respectively. All EOs exhibited low cytotoxicity to murine macrophages. The results presented here point to a promising possibility for the use S. terebinthifolius EOs as an alternative to chemotherapy treatment against L. infantum parasite and consequently, against visceral and post-kala-azar leishmaniasis.

Título do Evento: IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Título dos Anais do Evento: Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

SOUZA, Bianca Augusto de et al.. IN VITRO ANALYSIS OF THE LEISHMANICIDAL ACTIVITY AND TOXICITY OF THE ESSENTIAL OIL OF SCHINUS TEREBINTHIFOLIUS RADDI.. In: Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS). Anais...Seropédica(RJ) UFRRJ, 2025. Disponível em: https//www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1027206-IN-VITRO-ANALYSIS-OF-THE-LEISHMANICIDAL-ACTIVITY-AND-TOXICITY-OF-THE-ESSENTIAL-OIL-OF-SCHINUS-TEREBINTHIFOLIUS-R. Acesso em: 08/11/2025