INVESTIGATION OF HEMOPARASITES IN ANIMALS OF THE ORDER TESTUDINES BATSCH, 1788 UNDER CONDITIONS OF WILDLIFE, SCREENING CENTERS AND EX SITU

Maria izabel Fernandes Gouveia Pereira; Marcos Pereira da Silva Junior; Gilliard do Nascimento Ferreira; Natália Élida Nascimento Ribeiro; Mirian Cardinot; João Gabriel Ferreira Cabral; Beatriz Figueiredo de Oliveira; Bruna de Azevedo Baêta; Matheus Dias Cordeiro; Claudia Bezerra da Silva

Título do Trabalho

Autores

Maria izabel Fernandes Gouveia Pereira
Marcos Pereira da Silva Junior
Gilliard do Nascimento Ferreira
Natália Élida Nascimento Ribeiro
Mirian Cardinot
João Gabriel Ferreira Cabral
Beatriz Figueiredo de Oliveira
Bruna de Azevedo Baêta
Matheus Dias Cordeiro
Claudia Bezerra da Silva

Modalidade

Resumo

Área temática

Outras áreas temáticas

Data de Publicação

10/03/2025

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1027143-investigation-of-hemoparasites-in-animals-of-the-order-testudines-batsch-1788-under-conditions-of-wildlife-scr

ISBN

978-65-272-1246-1

Palavras-Chave

hemoparasites, Testudines, Wildlife, PCR

Resumo

Introduction: The increase in illegal animal trafficking has intensified the proximity of reptiles to humans and domestic environments, increasing the risk of transmission of parasites that directly affect the health of Testudines. The record of seizures by law enforcement agencies confirms these findings. In reptiles, parasites from the Apicomplexa phylum, such as Hepatozoon spp. and Haemogregarina spp., are commonly observed and can compromise the health status of these animals, especially in captive situations. In response to this scenario, it is becoming increasingly relevant to diagnose and characterize hemoparasites in animals of the Testudines order, generating valuable information for the control of parasitic infections and the conservation of these reptiles. Aim: The main objective of this study was to investigate, diagnose and identify the hemoparasites that infect Testudines (turtles, tortoises and tortoises) from different conditions: free-living, captivity and sorting centers, considering the different environmental and management contexts; determining the diversity and abundance of these hemoparasites and characterizing them from a morphological and molecular point of view. Methods: This study will be conducted at the Laboratory for the Study of Parasite-Host Interaction (LEIPH) and the Laboratory of Hemoparasites and Vectors (LHV) of the Federal Rural University of Rio de Janeiro (UFRRJ). Sampling will be carried out in zoos, Wild Animal Screening Centers (CETAS) and coastal areas of Rio Grande do Norte and Rio de Janeiro, in partnership with sea turtle monitoring projects. To carry out this study, partnerships will be formalized between the institutions, and the project will be submitted to the UFRRJ Ethics Committee. Blood samples will be collected manually by venipuncture from animals at the selected sites, stored in a refrigerated environment in polystyrene tubes containing the anticoagulant EDTA (Ethylenediamine tetraacetic acid), sent to the laboratory and divided into two parts: one for making blood smears, which will be stained using the Giemsa method for microscopic analysis, and the other for molecular analysis. The morphological diagnosis will be carried out using optical microscopy, observing the presence of hemoparasites in the erythrocytes and recording their morphological variables. For the molecular analysis, deoxyribonucleic acid (DNA) from the samples will be extracted and amplified by Polymerase Chain Reaction (PCR) using specific primers for the Hepatozoon and Haemogregarina genus. The PCR products will then be subjected to electrophoresis and genetic sequencing for precise identification of the parasites. The characterization of the hemoparasites, including parasitemia, will be quantified and the morphometry will be detailed using microscopic and imaging analyses to document the alterations caused in the infected erythrocytes. Expected Results: the research could help in the conservation of these animals and in understanding the parasite-host relationship, benefiting the health of Testudines and minimizing the risk of transmission of zoonotic pathogens. In addition, the participation of UFRRJ students will allow practical training in parasitological techniques, promoting advances in scientific knowledge about the parasitology of these reptiles.

Título do Evento: IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Título dos Anais do Evento: Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

PEREIRA, Maria izabel Fernandes Gouveia et al.. INVESTIGATION OF HEMOPARASITES IN ANIMALS OF THE ORDER TESTUDINES BATSCH, 1788 UNDER CONDITIONS OF WILDLIFE, SCREENING CENTERS AND EX SITU.. In: Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS). Anais...Seropédica(RJ) UFRRJ, 2025. Disponível em: https//www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1027143-INVESTIGATION-OF-HEMOPARASITES-IN-ANIMALS-OF-THE-ORDER-TESTUDINES-BATSCH-1788-UNDER-CONDITIONS-OF-WILDLIFE-SCR. Acesso em: 10/11/2025