OCCURRENCE AND MOLECULAR CHARACTERIZATION OF HEMOPLASMAS IN SMALL NON-FLYING MAMMALS FROM AMAZON RAIN FOREST REGIONS OF ACRE, BRAZIL

Isaac Leandro Lira Pinto; Eduarda de Oliveira Silva Lima Machado; Thiago Dutra Dias; Laís Da Silva De Oliveira; Tatiana Pádua Tavares De Freitas; Letícia Pereira Da Silva; Victor Silva de Souza; Huarrisson Azevedo Santos; Bernardo Rodrigues Teixeira; Maristela Peckle Peixoto

Título do Trabalho

Autores

Isaac Leandro Lira Pinto
Eduarda de Oliveira Silva Lima Machado
Thiago Dutra Dias
Laís Da Silva De Oliveira
Tatiana Pádua Tavares De Freitas
Letícia Pereira Da Silva
Victor Silva de Souza
Huarrisson Azevedo Santos
Bernardo Rodrigues Teixeira
Maristela Peckle Peixoto

Modalidade

Resumo

Área temática

Protozoários e Agentes transmitidos por vetores de importância em Saúde

Data de Publicação

10/03/2025

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1025880-occurrence-and-molecular-characterization-of-hemoplasmas-in-small-non-flying-mammals-from-amazon-rain-forest-reg

ISBN

978-65-272-1246-1

Palavras-Chave

Rodentia; Didelphimorphia; Mycoplasma; PCR

Resumo

Hemotropic mycoplasmas, widely known as hemoplasmas, are epierythrocytic bacteria capable of infecting a wide variety of mammalian hosts, including wild animals and humans. Although the diversity of hemoplasma hosts has grown, studies that seek to clarify the effects, infection factors, and co-infections of this pathogen in wildlife remains limited. With that in mind, this study aimed to explore the molecular occurrence of hemoplasmas in rodents and marsupials sampled from regions of the Amazon rainforest surrounding urban areas of Rio Branco, Acre expecting to contribute to their molecular characterization. Samples of liver parenchyma from 43 wild rodents and 32 marsupials collected in November 2021 had their DNA extracted using a commercial kit. Quantification and quality assessment of extracted DNA was performed using the NanoDrop® spectrophotometer (Thermo Fisher Scientific). The species collected had their identity confirmed through a conventional PCR (cPCR) assay based on the endogenous Cytochrome B gene using primer L14724-CIT B - VER (1100pb). Additionally, cPCR assays using primers HemMycop16S-41s/HemMycop16S-938as and HemMycop16S-322s/HemMycop16S-1420as, which target a 1300pb fragment of the 16S rDNA gene of Mycoplasma spp. were performed. The PCR products were subjected to electrophoresis using agarose gel at a concentration of 1.5%, stained with ethidium bromide and observed in a photodocumenter. Samples were purified and then sequenced using Sanger method. The assembled contigs were aligned with 47 Mycoplasma spp. sequences deposited in Genbank using ClustaW method. In addition, a sequence of Mycoplasma mirougirhinis (NR181869) was used as an outgroup. The phylogenetic tree was built using the Maximum Likelihood method and the Tamura-Nei+G evolutionary model, based on an alignment of 947pb of the 16S rDNA gene. The combination of phylogenetic groups was evaluated by testing 1000 replicates of “Bootstrap”. Positivity rate for hemoplasmas was 4.65% (2/43) among rodents and 3.12% (1/32) among marsupials. Positive samples were obtained from Proechimys simonsi (ID 22084), Proechimys brevicauda (ID 22089) and Philander canus (ID 22141). In the BLASTn analysis samples #22084 and #22089 presented respectively 97.54% and 98.29% identity with Mycoplasma sp. previously identified in a wild rodent (KT215623) from Brazil. While the sample #22141 demonstrated a similarity of 98.75% with a sequence of Mycoplasma formerly identified in a Didelphis albiventris (MN423258) from Brazil. In the phylogenetic analysis, samples #22084 and #22089 were placed in the haemofelis group and shared the same clade as Mycoplasma spp. previously identified in rodents from Brazil, while sample #22141 was placed in the suis group and shared a clade with Mycoplasma sequences from didelphids collected in Brazil and USA. Despite the low positivity rates for hemoplasmas, the phylogenetic analyses revealed significant genetic relationships between the identified mycoplasmas and previously documented strains in Brazil, contributing to the understanding of the ecology and transmission dynamics of these pathogen. This study highlights the need for further studies to explore the broader implications of hemoplasma infections in wildlife, particularly in regions undergoing rapid urbanization, where the interaction between wildlife and human environments may facilitate pathogen emergence and co-infection events.

Título do Evento: IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Título dos Anais do Evento: Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

PINTO, Isaac Leandro Lira et al.. OCCURRENCE AND MOLECULAR CHARACTERIZATION OF HEMOPLASMAS IN SMALL NON-FLYING MAMMALS FROM AMAZON RAIN FOREST REGIONS OF ACRE, BRAZIL.. In: Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS). Anais...Seropédica(RJ) UFRRJ, 2025. Disponível em: https//www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1025880-OCCURRENCE-AND-MOLECULAR-CHARACTERIZATION-OF-HEMOPLASMAS-IN-SMALL-NON-FLYING-MAMMALS-FROM-AMAZON-RAIN-FOREST-REG. Acesso em: 10/11/2025