EFFECT OF ESSENTIAL OILS FROM ORIGANUM VULGARE (L.) AND THYMUS VULGARIS (L.) AND THEIR RESPECTIVE ISOLATED COMPOUNDS CARVACROL AND THYMOL ON STOMOXYS CALCITRANS, THE STABLE FLY.

FELLIPE MATHEUS SOUZA DA COSTA; Jamyle Marques Vieira; Yara Peluso Cid; Patrícia Fampa Negreiros Lima

Título do Trabalho

Autores

FELLIPE MATHEUS SOUZA DA COSTA
Jamyle Marques Vieira
Yara Peluso Cid
Patrícia Fampa Negreiros Lima

Modalidade

Resumo

Área temática

Ectoparasitos: sistemática, epidemiologia e controle

Data de Publicação

10/03/2025

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1016540-effect-of-essential-oils-from-origanum-vulgare-(l)-and-thymus-vulgaris-(l)-and-their-respective-isolated-compo

ISBN

978-65-272-1246-1

Palavras-Chave

Stomoxys calcitrans, Orégano, Tomilho, Carvacrol, Óleo essencial

Resumo

Stomoxys calcitrans, the stable fly, is an obligate hematophagous insect. Both sexes feed on blood for survival and reproduction. It is a pest of livestock worldwide, with a preference for bovine blood. Its parasitism reduces the health and well-being of the animals, as the bite is painful. Besides, stable fly is a mechanical vector for several pathogens, causing great economic losses to the meat and dairy industry. Insecticides are routinely used to control the ectoparasite S. calcitrans, which causes significant environmental problems such as the emergence of resistant populations of various classes of insecticides and the environmental deposition of these chemicals. Essential oils of plant origin present alternatives to the use of insecticides in the control of these pests and have the advantage of being environmentally friendly. The present study aims to evaluate the effects of essential oils of Origanum vulgare L. and Thymus vulgaris L on S. calcitrans and their major constituents carvacrol and thymol, These compounds at different concentrations were topically applied in flies’ abdomen to monitor their longevity, comparing the longevity of males and females after topical application, monitoring the behavior. The 50% lethal dose (LD50) and the 90% lethal dose (LD90) were calculated. Two hundred and fifty six adult flies, 24h post emergence, were anesthetized in a Petri dish positioned over ice. With the abdomen exposed upwards, 1 microliter of the essential oils of thyme and oregano, and of the major compounds carvacrol and thymol were applied to the abdomen of the flies. They were kept in cages with 8 flies, with males and females at 1:1, in each cage with concentration groups of 2500 ppm, 3500 ppm, 4000 ppm, 5000 ppm, 10000 ppm, 20000 ppm, 35000 ppm and 50000 ppm, in addition to the control group treated with acetone alone. The flies were kept feeding with bovine blood throughout the experiment. The longevity of the flies after topical application was daily monitored. The highest concentrations of 35,000 ppm and 50,000 ppm, killed all 8 flies in 24 hours, and the lowest concentrations, 2,500 ppm and 4,000 ppm, had a survival pattern similar to the 28-day acetone control group, demonstrating the dose-dependent action of essential oils. A difference between the sexes was observed, as at the highest concentrations and at the intermediate concentration of 5000 ppm, males died more quickly than females, and the LD50 and LD90 of oregano essential oil were calculated with values of 4424.65 ppm and 12721.48 ppm after 24 hours and 3366.03 ppm and 8690.35 ppm after 48 hours. Changes in the flies’ behavior, such as in the movements of the legs, wings, and loss of balance, were observed after treatment with the essential oils and their major components (Costa et al., 2023). Based on our results, oregano and thyme essential oils may constitute a promising alternative to commercial insecticides used to control stable flies. As future perspectives, the impact of the compounds on the life cycle of S. calcitrans will be investigated, monitoring reproductive parameters such as oviposition, fertility and fecundity and also on the development in immature and adult stages. Posteriorly, a pharmaceutical formulation will be produced with the compound that presents the best performance for in vivo application.

Título do Evento: IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Título dos Anais do Evento: Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

COSTA, FELLIPE MATHEUS SOUZA DA et al.. EFFECT OF ESSENTIAL OILS FROM ORIGANUM VULGARE (L.) AND THYMUS VULGARIS (L.) AND THEIR RESPECTIVE ISOLATED COMPOUNDS CARVACROL AND THYMOL ON STOMOXYS CALCITRANS, THE STABLE FLY... In: Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS). Anais...Seropédica(RJ) UFRRJ, 2025. Disponível em: https//www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1016540-EFFECT-OF-ESSENTIAL-OILS-FROM-ORIGANUM-VULGARE-(L)-AND-THYMUS-VULGARIS-(L)-AND-THEIR-RESPECTIVE-ISOLATED-COMPO. Acesso em: 15/11/2025