EFFECTS OF CADMIUM, COBALT AND MANGANESE ON SPLICING AND BIOSYNTHESIS OF THE SPLICEOSOME IN THE AQUATIC FUNGUS BLASTOCLADIELLA EMERSONII

LETICIA HARUMI OSHIQUIRI; Suely Lopes Gomes; Raphaela de Castro Georg

Paper Title

Authors

LETICIA HARUMI OSHIQUIRI
Suely Lopes Gomes
Raphaela de Castro Georg

Modality

Abstract

Subject area

Stress mechanisms and responses in fungi: molecular biology, biochemistry, biophysics, and cellular biology

Publishing Date

15/05/2019

Country of Publishing

Brasil

Language of Publishing

Inglês

Paper Page

www.even3.com.br/Anais/ISFUS/160301-EFFECTS-OF-CADMIUM-COBALT-AND-MANGANESE-ON-SPLICING-AND-BIOSYNTHESIS-OF-THE-SPLICEOSOME-IN-THE-AQUATIC-FUNGUS-BL

ISBN

978-85-5722-210-6

Keywords

cadmium, cobalt, manganese, splicing, spliceosome

Summary

In eukaryotes, splicing is an essential process, catalyzed by the spliceosome complex, which occurs co-transcriptionally, involving removal of non-coding regions (introns) and joining of coding regions (exons) of pre-mRNAs. In the aquatic fungus Blastocladiella emersonii, some stressful conditions, as cadmium exposure, can cause inhibition of splicing. The mechanism underlying this effect remains unclear. In the present study, we used RT-qPCR technique to investigate the level of spliced and unspliced transcripts in B. emersonii genes that are known to be highly expressed under cadmium stress and genes that are known to be highly expressed under normal conditions, in order to better understand if the inhibition occurs randomly and therefore is more frequent on highly expressed, abundant, genes. We also performed gene expression assays using different concentrations of cobalt and manganese to verify if the inhibition is triggered by other divalent metals that share similar properties with cadmium. To determine the effect of the metals on the biosynthesis of the spliceosome, we analyzed the expression of some of its components: the snRNAs U1, U2, U4, U5 and U6 under the same conditions. Our results showed that splicing was inhibited in both groups of genes and the metals cobalt and manganese only slightly affected the unspliced levels of transcripts compared to cadmium. The snRNAs U1, U5 and U6 were strongly up-regulated in response to the cadmium treatment, while U2 and U4 were down-regulated. Cobalt treatment up-regulated U1 and U5 snRNA levels and manganese treatment down-regulated U2 snRNA levels. It seems that the levels of snRNAs are probably raised as a compensatory way to overcome the negative effects of splicing inhibition and to enable its survival in the hostile environment. Together, these data suggest that although these metals share common characteristics, the splicing process is more strongly inhibited by cadmium, a non-essential heavy metal.

Title of the Event: III International Symposium on Fungal Stress – ISFUS
City of the Event: São José dos Campos
Title of the Proceedings of the event: Annals of the International Symposium on Fungal Stress – ISFUS
Name of the Publisher: Even3
Means of Dissemination: Meio Digital
DOI: Get DOI

How to cite

OSHIQUIRI, LETICIA HARUMI; GOMES, Suely Lopes; GEORG, Raphaela de Castro. EFFECTS OF CADMIUM, COBALT AND MANGANESE ON SPLICING AND BIOSYNTHESIS OF THE SPLICEOSOME IN THE AQUATIC FUNGUS BLASTOCLADIELLA EMERSONII.. In: Annals of the International Symposium on Fungal Stress – ISFUS. Anais...São José dos Campos(SP) Hotel Nacional Inn, 2019. Available in: https//www.even3.com.br/anais/ISFUS/160301-EFFECTS-OF-CADMIUM-COBALT-AND-MANGANESE-ON-SPLICING-AND-BIOSYNTHESIS-OF-THE-SPLICEOSOME-IN-THE-AQUATIC-FUNGUS-BL. Access in: 27/04/2024