EFFECTS OF PHOTOBIOMODULATION ON HUMAN PLATELETS: CHARACTERIZATION OF THE ABSORPTION SPECTRUM AND ANALYSIS OF CELL VIABILITY

Eliane de Queiroz Lima Cruz; Henrique Roman Ramos; Rebeca Boltes; Alfredo Mendrone Junior; Sandra Kalil Bussadori; Anna Carolina Ratto Tempestinni Horliana; Maria Fernanda Setubal Destro Rodrigues; Kristianne Porta Santos Fernandes; Raquel Agnelli Mesquita Ferrari; Cinthya Duran

Título del Trabajo

Autores

Eliane de Queiroz Lima Cruz
Henrique Roman Ramos
Rebeca Boltes
Alfredo Mendrone Junior
Sandra Kalil Bussadori
Anna Carolina Ratto Tempestinni Horliana
Maria Fernanda Setubal Destro Rodrigues
Kristianne Porta Santos Fernandes
Raquel Agnelli Mesquita Ferrari
Cinthya Duran

Modalidad

Investigación

Área temática

Biomedicina

Fecha de publicación

14/08/2026

País de publicación

Argentina | Argentina

Idioma de la Publicación

es-419

Página del Trabajo

https://www.even3.com.br/anais/ii-encuentro-biofotonica-597461/1258129-effects-of-photobiomodulation-on-human-platelets--characterization-of-the-absorption-spectrum-and-analysis-of-ce

ISBN

978-65-272-2678-9

Palabras Clave

photobiomodulation, platelets, cell viability

Resumen

Introduction: Human platelets play an essential role in hemostasis, immune response, and tissue repair processes. However, during storage, they undergo biochemical and functional changes that may compromise their therapeutic quality in transfusion medicine. Strategies aimed at preserving platelet viability and functionality are highly relevant. Photobiomodulation (PBM)—the application of low-intensity light to modulate biological processes—is considered a promising approach to maintaining cell functionality during storage. Objective: To characterize the absorption spectrum of human platelets and evaluate the effects of PBM on cell viability. Methodology: Platelet concentrates obtained by apheresis from healthy donors will be provided by the Fundação Pró-Sangue (São Paulo Blood Center Foundation), School of Medicine, University of São Paulo, Brazil. The dosimetric parameters for each irradiated group will be defined based on the initial spectrophotometric analysis. Each experimental group will be subjected to the following evaluations: absorption spectrophotometry; cell viability (trypan blue exclusion method); platelet morphology (optical microscopy); nitric oxide dosage (Griess method) and total protein quantification (Bradford method); and apoptosis analysis (Annexin staining by flow cytometry). This project will be approved by the Research Ethics Committee of Universidade Nove de Julho (opinion No. 7,619,499).

Título del Evento: II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Ciudad del Evento: Buenos Aires
Título de las Actas del Evento: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Nombre de la Editora: Even3
Medio de Divulgación: Meio Digital

Como citar

CRUZ, Eliane de Queiroz Lima et al.. EFFECTS OF PHOTOBIOMODULATION ON HUMAN PLATELETS: CHARACTERIZATION OF THE ABSORPTION SPECTRUM AND ANALYSIS OF CELL VIABILITY.. In: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud. Anais...Sao Paulo(SP) UNIMAIMONIDES, 2026. Disponible en: https//www.even3.com.br/anais/II-Encuentro-Biofotonica-597461/1258129-EFFECTS-OF-PHOTOBIOMODULATION-ON-HUMAN-PLATELETS--CHARACTERIZATION-OF-THE-ABSORPTION-SPECTRUM-AND-ANALYSIS-OF-CE. Acceso en: 21/08/2026