EFFECT OF PHOTOBIOMODULATION IN AN IN VITRO EXPERIMENTAL MODEL OF ASTHMA AND COPD OVERLAP SYNDROME

Isadora Nilsson Mechenas; Marina Bertoni Guerra; Tawany Gonçalves Santos; Cintia Estefano Alves Silva; Ana Paula LIgeiro de Oliveira

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Trabajo

Título del Trabajo

EFFECT OF PHOTOBIOMODULATION IN AN IN VITRO EXPERIMENTAL MODEL OF ASTHMA AND COPD OVERLAP SYNDROME

Autores

Isadora Nilsson Mechenas
Marina Bertoni Guerra
Tawany Gonçalves Santos
Cintia Estefano Alves Silva
Ana Paula LIgeiro de Oliveira

Modalidad

Investigación

Área temática

Medicina

Fecha de publicación

14/08/2026

País de publicación

Argentina | Argentina

Idioma de la Publicación

es-419

Página del Trabajo

https://www.even3.com.br/anais/ii-encuentro-biofotonica-597461/1255095-effect-of-photobiomodulation-in-an-in-vitro-experimental-model-of-asthma-and-copd-overlap-syndrome

ISBN

978-65-272-2678-9

Palabras Clave

asthma-COPD overlap syndrome, ACOS, photobiomodulation therapy

Resumen

Asthma and COPD (Chronic Obstructive Pulmonary Disease) are chronic inflammatory conditions with high prevalence in the global population. Asthma-COPD Overlap Syndrome (ACOS) can be diagnosed in patients who exhibit features of both conditions. However, the clinical characteristics are not yet fully recognized or clearly defined. Treatment is based on behavioral interventions, along with pharmacological approaches, primarily involving the use of inhaled glucocorticoids. Photobiomodulation therapy (PBM) is known to have beneficial effects on lung diseases. This study aimed to evaluate the effects of PBM on the inflammatory process in an in vitro experimental model of ACOS. Cells were plated (5 x 10⁴ cells/well) in 24-well plates. Six experimental groups (n=4) were established using human bronchial epithelial cells (BEAS-2B). After 24 hours of culture, the cells were incubated or not with cigarette smoke extract (CSE) 100μl (2.5%) and house dust mite (HDM) 1𝜇g/ml. After 1 hour, the plates were irradiated with a diode laser (660nm, 30mW, 5,4J, 180s) or dexamethasone (DEXA) diluted in DMSO (dimethyl sulfoxide) 10-⁷ M. The groups were divided into: Baseline group; CSE + HDM - positive control (ACOS group); DEXA-treated cells; Laser-irradiated cells; CSE + HDM + DEXA group; CSE + HDM + LASER group. After 24 hours, cytokine levels were quantified and a reduction (p<0,05) was observed in the levels of IL-1β, IL-6, TNF-α, IL-4, IL-5, IL-13, IL-17, IL-21 and IL-23, along with an increase (p<0,05) in IL-10 and IFN-γ in laser-irradiated ACOS group cells compared to the positive control group. These results suggest that photobiomodulation may beneficially modulate the inflammation of ACOS. However, further studies are needed to consider PBM as a potential therapeutic option for this condition.

Título del Evento: II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Ciudad del Evento: Buenos Aires
Título de las Actas del Evento: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Nombre de la Editora: Even3
Medio de Divulgación: Meio Digital

Como citar

MECHENAS, Isadora Nilsson et al.. EFFECT OF PHOTOBIOMODULATION IN AN IN VITRO EXPERIMENTAL MODEL OF ASTHMA AND COPD OVERLAP SYNDROME.. In: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud. Anais...Sao Paulo(SP) UNIMAIMONIDES, 2026. Disponible en: https//www.even3.com.br/anais/II-Encuentro-Biofotonica-597461/1255095-EFFECT-OF-PHOTOBIOMODULATION-IN-AN-IN-VITRO-EXPERIMENTAL-MODEL-OF-ASTHMA-AND-COPD-OVERLAP-SYNDROME. Acceso en: 21/08/2026