EFFECTS OF PHOTOBIOMODULATION COMBINED OR NOT WITH DEXAMETHASONE ON VIABILITY AND INFLAMMATORY MARKERS IN M1 MACROPHAGES

Rosani Tereza De Siqueira E Silva; Tainá Caroline dos Santos Malavazzi; Alessandra Lima da Silva Martins; Claudio Teruo Kassa; Aline Souza Silva; Sandra Kalil Bussadori; Anna Carolina Ratto Tempestinni Horliana; Kristianne Porta Santos Fernandes; Raquel Agnelli Mesquita Ferrari; Cinthya Cosme Gutierrez Duran

Título del Trabajo

Autores

Rosani Tereza De Siqueira E Silva
Tainá Caroline dos Santos Malavazzi
Alessandra Lima da Silva Martins
Claudio Teruo Kassa
Aline Souza Silva
Sandra Kalil Bussadori
Anna Carolina Ratto Tempestinni Horliana
Kristianne Porta Santos Fernandes
Raquel Agnelli Mesquita Ferrari
Cinthya Cosme Gutierrez Duran

Modalidad

Investigación

Área temática

Medicina

Fecha de publicación

14/08/2026

País de publicación

Argentina | Argentina

Idioma de la Publicación

es-419

Página del Trabajo

https://www.even3.com.br/anais/ii-encuentro-biofotonica-597461/1253773-effects-of-photobiomodulation-combined-or-not-with-dexamethasone-on-viability-and-inflammatory-markers-in-m1-mac

ISBN

978-65-272-2678-9

Palabras Clave

Inflammation, Photobiomodulation, Dexamethasone

Resumen

INTRODUCTION: Inflammation is essential for tissue defense and repair. However, its dysregulation may lead to chronic diseases. Macrophages play a central role, polarizing into M1 (pro-inflammatory) or M2 (anti-inflammatory) phenotypes. Dexamethasone (DEXA) is widely used to control inflammation but may cause cytotoxicity. Photobiomodulation (PBM), using low-level laser therapy, has shown potential in modulating inflammatory responses with fewer side effects.OBJECTIVE: To evaluate, in vitro, the effects of PBM, DEXA, and their combination on cell viability and the expression of TNF-α and IL-6 in J774 macrophages activated to the M1 profile.METHODOLOGY: J774 macrophages were divided into seven experimental groups: (1) Control, (2) M1, (3) M1 + PBM, (4) M1 + DEXA 2 µM, (5) M1 + DEXA 4 µM, (6) M1 + PBM + DEXA 2 µM, (7) M1 + PBM + DEXA 4 µM. Activation was performed using LPS (1 µg/mL) and IFN-γ (0.2 µg/mL). PBM was applied using a 780 nm laser (70 mW, 17.5 J/cm², 1 J). Cell viability was assessed by MTT and cytokines by ELISA at 24 and 48 h.RESULTS: At 24 h, the PBM + DEXA 2 µM group showed significantly higher cell viability than DEXA alone, while DEXA 4 µM reduced total protein content. Both DEXA concentrations decreased TNF-α and IL-6 levels; however, PBM partially mitigated these anti-inflammatory effects.CONCLUSION: The combination of PBM with DEXA 2 µM showed a synergistic effect, balancing anti-inflammatory action with better cell viability. These findings support the potential use of PBM as a coadjuvant to glucocorticoid therapy. As a continuation in the PhD project, gene expression analysis of cytokines will be performed using PCR to complement and deepen the inflammatory profile findings.

Título del Evento: II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Ciudad del Evento: Buenos Aires
Título de las Actas del Evento: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Nombre de la Editora: Even3
Medio de Divulgación: Meio Digital

Como citar

SILVA, Rosani Tereza De Siqueira E et al.. EFFECTS OF PHOTOBIOMODULATION COMBINED OR NOT WITH DEXAMETHASONE ON VIABILITY AND INFLAMMATORY MARKERS IN M1 MACROPHAGES.. In: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud. Anais...Sao Paulo(SP) UNIMAIMONIDES, 2026. Disponible en: https//www.even3.com.br/anais/II-Encuentro-Biofotonica-597461/1253773-EFFECTS-OF-PHOTOBIOMODULATION-COMBINED-OR-NOT-WITH-DEXAMETHASONE-ON-VIABILITY-AND-INFLAMMATORY-MARKERS-IN-M1-MAC. Acceso en: 21/08/2026