PROTECTIVE EFFECTS OF PHOTOBIOMODULATION AGAINST BOTHROPS ALTERNATUS VENOM TOXICITY IN C2C12 MYOBLASTS: DIFFERENTIAL RESPONSES TO JUVENILE VS ADULT VENOMS

Alecsandra Oliveira Merino; Stella Regina Zamuner; Amanda Cabral David; María Emília García Denegri; Laura Cristina A. Leiva; Juliana Pavan Zuliani

Título del Trabajo

Autores

Alecsandra Oliveira Merino
Stella Regina Zamuner
Amanda Cabral David
María Emília García Denegri
Laura Cristina A. Leiva
Juliana Pavan Zuliani

Modalidad

Investigación

Área temática

Farmacia

Fecha de publicación

14/08/2026

País de publicación

Argentina | Argentina

Idioma de la Publicación

es-419

Página del Trabajo

https://www.even3.com.br/anais/ii-encuentro-biofotonica-597461/1253656-protective-effects-of-photobiomodulation-against-bothrops-alternatus-venom-toxicity-in-c2c12-myoblasts--differen

ISBN

978-65-272-2678-9

Palabras Clave

photobiomodulation, inflammation, edema, hyperalgesia, Brothpsatrox.

Resumen

Snakes of the genus Bothrops hold significant medical and epidemiological relevance, as their envenomation induces severe local and systemic effects in victims. Additionally, variations in venom composition between juvenile and adult snakes may result in distinct clinical outcomes. Photobiomodulation (PBM) has been shown to be an effective adjuvant therapy to serum therapy to reduce the local effects induced by bothropic snake venom. This study evaluated the effect of PBM on C2C12 myoblast cells after exposition of Bothrops alternatus snake venom (BaV) in its juvenile (BaJV) and adult (BaAV) stages. C2C12 cells received VBaJ or VBaA (50 µg/mL), or only culture medium (control), and were immediately treated with PBM. Treatment was performed using a low-level laser (660 nm wavelength, 4 J/cm², 100 mW, 0.33 W/cm², 40 seconds). Cell viability, and cell proliferation and differentiation were assessed 3 hours after venom exposure using MTT assays and flow cytometry. Results showed that both venoms caused a decrease in cell viability and cell differentiation. PBM treatment caused increases in cell differentiation and proliferation, but had no effect on cell viability. It can be concluded that, although both venoms (juvenile and adult) are capable of causing effects on C2C12 cells, there is a difference in the magnitude of some of these effects, and treatment with PBM proved to be beneficial in protecting C2C12 cells against the venoms of B. alternatus in its different stages of snake development.

Título del Evento: II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Ciudad del Evento: Buenos Aires
Título de las Actas del Evento: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud
Nombre de la Editora: Even3
Medio de Divulgación: Meio Digital

Como citar

MERINO, Alecsandra Oliveira et al.. PROTECTIVE EFFECTS OF PHOTOBIOMODULATION AGAINST BOTHROPS ALTERNATUS VENOM TOXICITY IN C2C12 MYOBLASTS: DIFFERENTIAL RESPONSES TO JUVENILE VS ADULT VENOMS.. In: Actas II Encuentro Latinoamericano de Investigación y Práctica en Biofotónica y I Seminario de Investigación de Posgrado en Salud. Anais...Sao Paulo(SP) UNIMAIMONIDES, 2026. Disponible en: https//www.even3.com.br/anais/II-Encuentro-Biofotonica-597461/1253656-PROTECTIVE-EFFECTS-OF-PHOTOBIOMODULATION-AGAINST-BOTHROPS-ALTERNATUS-VENOM-TOXICITY-IN-C2C12-MYOBLASTS--DIFFEREN. Acceso en: 21/08/2026